全文获取类型
收费全文 | 2209篇 |
免费 | 5篇 |
专业分类
电工技术 | 2篇 |
综合类 | 1篇 |
化学工业 | 31篇 |
金属工艺 | 3篇 |
机械仪表 | 12篇 |
建筑科学 | 8篇 |
矿业工程 | 12篇 |
能源动力 | 5篇 |
轻工业 | 21篇 |
水利工程 | 1篇 |
无线电 | 33篇 |
一般工业技术 | 29篇 |
冶金工业 | 2002篇 |
原子能技术 | 3篇 |
自动化技术 | 51篇 |
出版年
2022年 | 3篇 |
2021年 | 2篇 |
2019年 | 2篇 |
2018年 | 2篇 |
2017年 | 2篇 |
2016年 | 5篇 |
2013年 | 17篇 |
2012年 | 6篇 |
2011年 | 10篇 |
2010年 | 5篇 |
2009年 | 11篇 |
2008年 | 9篇 |
2007年 | 16篇 |
2006年 | 6篇 |
2005年 | 8篇 |
2004年 | 12篇 |
2003年 | 15篇 |
2002年 | 9篇 |
2001年 | 7篇 |
2000年 | 4篇 |
1999年 | 68篇 |
1998年 | 663篇 |
1997年 | 341篇 |
1996年 | 233篇 |
1995年 | 119篇 |
1994年 | 117篇 |
1993年 | 114篇 |
1992年 | 15篇 |
1991年 | 21篇 |
1990年 | 29篇 |
1989年 | 34篇 |
1988年 | 30篇 |
1987年 | 21篇 |
1986年 | 17篇 |
1985年 | 20篇 |
1984年 | 2篇 |
1983年 | 5篇 |
1982年 | 13篇 |
1981年 | 14篇 |
1980年 | 21篇 |
1979年 | 2篇 |
1978年 | 7篇 |
1977年 | 42篇 |
1976年 | 94篇 |
1975年 | 3篇 |
1970年 | 2篇 |
1959年 | 1篇 |
1955年 | 3篇 |
1947年 | 1篇 |
1946年 | 1篇 |
排序方式: 共有2214条查询结果,搜索用时 0 毫秒
71.
JK Osbourn JC Earnshaw KS Johnson M Parmentier V Timmermans J McCafferty 《Canadian Metallurgical Quarterly》1998,16(8):778-781
The seven trans-membrane chemokine receptor CCR-5 is a coreceptor for macrophage tropic HIV-1 strains. CCR-5 responds to a number of chemokines, including macrophage inflammatory protein (MIP)-1 alpha. We describe the use of MIP-1 alpha in a biotin tyramine-mediated proximity selection to guide the selection of CCR-5-specific phage antibodies from a large phage display human library. Proximity based selections resulted in a population of antibodies recognizing CCR-5 on primary CD4+ lymphocytes, none of which blocked MIP-1 alpha binding to cells. The selected population of phage antibodies were subsequently used as guide molecules for a second phase of selection that was carried out in the absence of MIP-1 alpha. This generated a panel of CCR-5-binding antibodies, of which around 20% inhibited MIP-1 alpha binding to CD4+. The single chain Fvs (scFv) generated by this step-back selection procedure also inhibited MIP-1 alpha-mediated calcium signaling. 相似文献
72.
73.
GB Scarfe B Wright E Clayton S Taylor ID Wilson JC Lindon JK Nicholson 《Canadian Metallurgical Quarterly》1998,28(4):373-388
74.
This paper presents summaries of initiatives and demonstration programs developed by professional and patient care organizations to improve care at the end of life. 相似文献
75.
Genotoxic stress triggers signalling pathways that either mediate cell killing or protection of affected cells. While induction of p53 is observed for most of the genotoxins, activation of MAPK/SAPK cascades is not a general response. The role of MAPK/SAPK activation on cell fate, seems to be dependent, in some systems, on the balanced response among both cascades. We have here examined the effect of cis and trans-DDP on the activation of ERK and JNK activities. While no significant induction of ERK was observed with the compounds, both of them are able to strongly activate JNK. Trans-DDP response is rapid and transient while the cis-DDP one is slow and persistent. In contrast with the observed nuclear translocation of JNK in response to U.V. light, none of the platinum compounds induces translocation, on the contrary, activation of JNK occurs in both the nuclear and cytoplasmic compartments. Inhibition of tyrosine phosphatases by orthovanadate pretreatment prolongs the time of JNK induction in response to both platinum compounds. The positive modulation of JNK activation correlates with an increase in toxicity that, for cis-DDP corresponds to a tenfold decrease in the IC50. A strong increase in MKP-1 levels was observed only in response to trans-DDP suggesting the involvement of this activity in the downregulation of JNK activity in response to this compound. Altogether the results suggest that the prolonged activation of JNK in response to cis-DDP contributes to cell death induction. 相似文献
76.
We compared the dose-response relationships of cisatracurium, mivacurium, atracurium, vecuronium and rocuronium and examined the interactions of cisatracurium with mivacurium, atracurium, vecuronium and rocuronium in humans by isobolographic and fractional analyses. We studied 180 adult patients during nitrous oxide-fentanyl-propofol anaesthesia. Neuromuscular block was monitored using mechanomyography to detect the twitch response of the ulnar nerve at the wrist. The dose-response curves were determined by probit analysis. The calculated ED50 values and their 95% confidence intervals were 40.9 (38.1-43.7), 49.8 (47.0-52.6), 187.2 (175.1-199.3), 36.6 (34.7-38.5) and 136.4 (129.2-143.6) micrograms.kg-1 for cisatracurium, mivacurium, atracurium, vecuronium and rocuronium, respectively. Corresponding ED95 values were 57.6 (53.5-61.7), 91.8 (88.1-95.5), 253.1 (238.9-267.3), 52.9 (49.1-56.7) and 288.7 (276.2-301.2) micrograms.kg-1, respectively. The interaction between cisatracurium and mivacurium, vecuronium or rocuronium was found to be synergistic, but the interaction between cisatracurium and atracurium was found to be additive. Synergy between cisatracurium and vecuronium or rocuronium was greater than between cisatracurium and mivacurium. 相似文献
77.
MS Lim SE Straus JK Dale TA Fleisher M Stetler-Stevenson W Strober MC Sneller JM Puck MJ Lenardo KS Elenitoba-Johnson AY Lin M Raffeld ES Jaffe 《Canadian Metallurgical Quarterly》1998,153(5):1541-1550
The defects in lymphocyte apoptosis that underlie the autoimmune lymphoproliferative syndrome (ALPS) are usually attributable to inherited mutations of the CD95 (Fas) gene. In this report, we present the histopathological and immunophenotypic features seen in the lymph nodes (n = 16), peripheral blood (n = 10), bone marrow (n = 2), spleen (n = 3), and liver (n = 2) from 10 patients with ALPS. Lymph nodes showed marked paracortical hyperplasia. Interfollicular areas were expanded and populated by T cell receptor-alphabeta CD3+ CD4-CD8- (double-negative, DN) T cells that were negative for CD45RO. CD45RA+ T cells were increased in all cases studied. The paracortical infiltrate was a result of both reduced apoptosis and increased proliferation, as measured by in situ detection of DNA fragmentation and staining with MIB-1, respectively. The paracortical proliferation may be extensive enough to suggest a diagnosis of malignant lymphoma. Many of the paracortical lymphocytes expressed markers associated with cytotoxicity, such as perforin, TIA-1, and CD57. CD25 was negative. In addition, most lymph nodes exhibited florid follicular hyperplasia, often with focal progressive transformation of germinal centers; in some cases, follicular involution was seen. A polyclonal plasmacytosis also was present. The spleens were markedly enlarged, more than 10 times normal size. There was expansion of both white pulp and red pulp, with increased DN T cells. DN T cells also were observed in liver biopsies exhibiting portal triaditis. In the peripheral blood, the T cells showed increased expression of HLA-DR and CD57 but not CD25. CD45RA+ T cells were increased in the four cases studied. Polyclonal B cell lymphocytosis with expansion of CD5+ B cells was a characteristic finding. Taken together, the histopathological and immunophenotypic findings, particularly in lymph nodes and peripheral blood, are sufficiently distinctive to suggest a diagnosis of ALPS. Of note, two affected family members of one proband developed lymphoma (T-cell-rich B-cell lymphoma and nodular lymphocyte predominance Hodgkin's disease, respectively). 相似文献
78.
79.
MJ Marttunen MM Henriksson ET Isomets? ME Heikkinen HM Aro JK L?nnqvist 《Canadian Metallurgical Quarterly》1998,33(131):669-681
The characteristics of male adolescent suicide victims with (n = 84) and without (n = 8) a diagnosable psychiatric disorder were compared. Using psychological autopsy methods--interviews with victims' family members and health care professionals, and review of records (e.g., medical, school, police)-data were collected on all adolescent suicides in Finland during a 12-month period. Compared with adolescents with a psychiatric disorder, those with no disorder tended to come from less disturbed families, had shown less antisocial behavior, and had less frequently utilized health care and social services. Adolescents with no disorder more often communicated suicidal thoughts for the first time just before the suicide, and difficulties with the law (discipline problems) were more common precipitants than among those with a disorder. It was concluded that the process leading to suicide seems to be relatively short among male adolescents with no diagnosable psychiatric disorder. Communication of suicidal intent and problems with discipline are among the few clinical warning signs. 相似文献
80.