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排序方式: 共有10000条查询结果,搜索用时 31 毫秒
31.
Manjunatha S. Muttigi Byoung Ju Kim Bogyu Choi Inbo Han Hansoo Park Soo-Hong Lee 《International journal of molecular sciences》2020,21(23)
Adipose-derived mesenchymal stromal cells (Ad-MSCs) are a promising tool for articular cartilage repair and regeneration. However, the terminal hypertrophic differentiation of Ad-MSC-derived cartilage is a critical barrier during hyaline cartilage regeneration. In this study, we investigated the role of matrilin-3 in preventing Ad-MSC-derived chondrocyte hypertrophy in vitro and in an osteoarthritis (OA) destabilization of the medial meniscus (DMM) model. Methacrylated hyaluron (MAHA) (1%) was used to encapsulate and make scaffolds containing Ad-MSCs and matrilin-3. Subsequently, the encapsulated cells in the scaffolds were differentiated in chondrogenic medium (TGF-β, 1–14 days) and thyroid hormone hypertrophic medium (T3, 15–28 days). The presence of matrilin-3 with Ad-MSCs in the MAHA scaffold significantly increased the chondrogenic marker and decreased the hypertrophy marker mRNA and protein expression. Furthermore, matrilin-3 significantly modified the expression of TGF-β2, BMP-2, and BMP-4. Next, we prepared the OA model and transplanted Ad-MSCs primed with matrilin-3, either as a single-cell suspension or in spheroid form. Safranin-O staining and the OA score suggested that the regenerated cartilage morphology in the matrilin-3-primed Ad-MSC spheroids was similar to the positive control. Furthermore, matrilin-3-primed Ad-MSC spheroids prevented subchondral bone sclerosis in the mouse model. Here, we show that matrilin-3 plays a major role in modulating Ad-MSCs’ therapeutic effect on cartilage regeneration and hypertrophy suppression. 相似文献
32.
随着市场的发展,精品超市和改造店铺逐渐增多,需要一种更加适合的制冷机组来满足市场的需求。本文提供了一种新型的解决方案,解决了现有机组存在的高噪音和高成本问题,不仅提高了制冷效率,而且节约能源,保护环境。 相似文献
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在国家政策和市场需求的双重推动下,BIM技术在国内建筑领域迎来快速发展阶段,从最初的概念普及,到能够局部应用,再到现在能够全过程应用,其趋势是十分明确的,但是在其发展的过程中仍然存在着不少问题,如传统技术与BIM技术深入融合问题,BIM技术如何解决工程施工现场实际问题等.本文主要研究BIM技术在连云港民用机场迁建工程旅客过夜用房酒店项目中的综合应用,从协同建模、碰撞检查、管线综合、施工图深化出图及漫游展示等方面着手,着重解决BIM技术在施工过程中应用的落地问题,从而达到提高效率,缩短工期,降低成本,提升质量的目标. 相似文献
36.
Elucidation of colour development and microstructural characteristics of Allium sativum fumigated with acetic acid 下载免费PDF全文
Li Li Dan Wang Xihong Li Yu Wang Xiaofeng Ju 《International Journal of Food Science & Technology》2015,50(5):1083-1088
It has been well known that the greening of Allium sativum cloves could be formed after immersed in acetic acid solution. Nonetheless, no investigation was reported on colour development of A. sativum in response to acetic acid vapour until now. In this study, the brief exposure of A. sativum to acetic acid vapour (200–400 ppm) was combined with controlled atmosphere (5%, 20% and 80% CO2) packaging storage to break cell membrane and green garlic. The garlic bulbs were fumigated with acetic acid before controlled CO2 atmosphere packaging for 25 days at 4 °C. Fumigation with 200 ppm acetic acid followed by high CO2 atmosphere packaging (80% CO2) facilitated the garlic greening. It was also verified that γ‐glutamyl transpeptidase was involved in garlic greening in present study, and the compromise of glacial acetic acid vapour fumigation and CO2 gas atmosphere in package stored at low temperature could result in garlic greening as well. 相似文献
37.
Mani Balamurugan Hui‐Yun Jeong Venkata Surya Kumar Choutipalli Jung Sug Hong Hongmin Seo Natarajan Saravanan Jun Ho Jang Kang‐Gyu Lee Yoon Ho Lee Sang Won Im Venkatesan Subramanian Sun Hee Kim Ki Tae Nam 《Small (Weinheim an der Bergstrasse, Germany)》2020,16(25)
The electrochemical reduction of carbon dioxide (CO2) to hydrocarbons is a challenging task because of the issues in controlling the efficiency and selectivity of the products. Among the various transition metals, copper has attracted attention as it yields more reduced and C2 products even while using mononuclear copper center as catalysts. In addition, it is found that reversible formation of copper nanoparticle acts as the real catalytically active site for the conversion of CO2 to reduced products. Here, it is demonstrated that the dinuclear molecular copper complex immobilized over graphitized mesoporous carbon can act as catalysts for the conversion of CO2 to hydrocarbons (methane and ethylene) up to 60%. Interestingly, high selectivity toward C2 product (40% faradaic efficiency) is achieved by a molecular complex based hybrid material from CO2 in 0.1 m KCl. In addition, the role of local pH, porous structure, and carbon support in limiting the mass transport to achieve the highly reduced products is demonstrated. Although the spectroscopic analysis of the catalysts exhibits molecular nature of the complex after 2 h bulk electrolysis, morphological study reveals that the newly generated copper cluster is the real active site during the catalytic reactions. 相似文献
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Wan Huan Wang Hui Scotney Bryan Liu Jun Ng Wing W. Y. 《Multimedia Tools and Applications》2020,79(39-40):29327-29352
Multimedia Tools and Applications - In many real-world classification problems there exist multiple subclasses (or clusters) within a class; in other words, the underlying data distribution is... 相似文献
40.
在广播电视行业不断发展条件下,电视专题节目制作也受到受众高度重视。这就应提高电视专题节目制作质量,在满足广大受众日常观看要求同时,使得我国广播电视行业向着现代化方向发展。针对电视专题节目制作进行研究,首先阐述电视专题节目制作特点,之后阐述电视专题节目制作质量问题和相关提升对策。在解决电视专题节目制作问题同时,提升电视专题节目制作质量,继而彰显电视专题节目制作优势。 相似文献