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41.
TL Holyoake MG Freshney L McNair AN Parker PJ McKay WP Steward E Fitzsimons GJ Graham IB Pragnell 《Canadian Metallurgical Quarterly》1996,87(11):4589-4595
The characterization of many cytokines involved in the control of hematopoiesis has led to intense investigation into their potential use in ex vivo culture to expand progenitor numbers. We have established the optimum ex vivo culture conditions that allow substantial amplification of transient engrafting murine stem cells and which, simultaneously, augment the ability to sustain serial bone marrow transplantation (BMT). Short-term incubation of unfractionated BM cells in liquid culture with stem cell factor (SCF) and interleukin-11 (IL-11) produced a 50-fold amplification of clonogenic multipotential progenitors (CFU-A). Following such ex vivo expansion, substantially fewer cells were required to rescue lethally irradiated mice. When transplanted in cell doses above threshold for engraftment, BM cells expanded ex vivo resulted in significantly more rapid hematopoietic recovery. In a serial transplantation model, unmanipulated BM was only able to consistently sustain secondary BMT recipients, but BM expanded ex vivo has sustained quaternary BMT recipients that remain alive and well more than 140 days after 4th degree BMT. These results show augmentation of both short-term recovery posttransplant and the ability to serially transplant marrow by preincubation in culture with SCF and IL-11. 相似文献
42.
Unilateral spatial neglect declines when participants reach to grip the center of long metal rods compared with when they point to the perceived center, suggesting that visuomotor control systems are less affected by neglect than other representational systems (I. H. Robertson, D. Nico, & B. Hood, 1995). In this study, 16 participants with unilateral left neglect actually picked up rods, and we predicted short-term improvements in neglect because of induced conflict between a phenomenally symmetrical visual world on the one hand and a proprioceptively conveyed rightward-biased world on the other. With participants serving as their own controls, significant short-term improvements in neglect were found on 2 out of 4 neglect tasks after participants experienced proprioceptive feedback discrepant from the judgments they made on the basis of visual information alone. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
43.
Roger Norman Rothon Christopher Mark Liauw Graham Clayton Lees Wayne Christopher Edward Schofield 《The Journal of Adhesion》2002,78(7):603-628
The interaction of ammonium stearate (AS) and γ-aminopropyltriethoxysilane (APS) treatments with a magnesium hydroxide flame retardant filler and their effects on its use in an ethylene vinyl acetate copolymer compound have been investigated. The work has shown clear evidence of changes in the structure of the surface layers formed on the filler as the amount of additive is increased and the levels at which these occur can be correlated with theoretical monolayer quantities. Infrared (IR) spectroscopy data suggest that the stearate coating changes from a half salt to a full salt as the coating level is increased and that the APS coating on the filler initially has a significant bicarbonate content, presumably due to reaction with atmospheric carbon dioxide. The effect of coating level on the melt flow rate, insoluble matrix content, crystallisation behaviour, tensile properties, limiting oxygen index, and ageing of the filled compound has been studied. Distinct trends have been observed, many of which can be correlated with the structure of the filler surface layers. Of particular importance is the observation that, unlike APS, excess stearate appears to promote detrimental ageing effects. 相似文献
44.
Tempest Philip R.; White Patricia; Williamson E.Diane; Titball Richard W.; Kelly David C.; Kemp Graham J.L.; Gray Peter M.D.; Forster Simon J.; Carr Frank J.; Harris William J. 《Protein engineering, design & selection : PEDS》1994,7(12):1501-1507
We have used the technique of antibody reshaping to producea humanized antibody specific for the a toxin of Clostridiumperfringens. The starting antibody was from a mouse hybridomafrom which variable (V) region nucleo-tide sequences were determined.The complementarity-determining regions (CDRs) from these Vregions were then inserted into human heavy and light chainV region genes with human constant region gene fragments subsequentlyadded. The insertion of CDRs alone into human frameworks didnot produce a functional reshaped antibody and modificationsto the V region framework were required. With minor frameworkmodifications, the affinity of the original murine mAb was restoredand even exceeded. Where affinity was increased, an alteredbinding profile to overlapping peptides was observed. Computermodelling of the reshaped heavy chain V regions suggested thatamino acids adjacent to CDRs can either contribute to, or distort,CDR loop conformation and must be adjusted to achieve high bindingaffinity. 相似文献
45.
Graham J. Holloway 《Journal of Stored Products Research》1985,21(4):165-169
Acid fuchsin staining is widely used as a method for easy detection of egg plugs deposited by Sitophilus spp. The effect of increasing grain moisture content through staining on some life history characters of three geographical strains of Sitophilus oryzae was examined. The results show that staining increases development rate and survival from egg to adult and there is also a suggestion of a strain × treatment interaction. Factors requiring consideration if staining is carried out are discussed. 相似文献
46.
47.
Phase Transformations in Dicalcium Silicate: II, TEM Studies of Crystallography, Microstructure, and Mechanisms 总被引:2,自引:0,他引:2
Young Joong Kim Ian Nettleship Waltraud M. Kriven 《Journal of the American Ceramic Society》1992,75(9):2407-2419
The crystallography, microstructures, and phase transformation mechanisms in dicalcium silicate (Ca2 SiO4 ) were studied by TEM. Three types of superlattice structures were observed in the α'L and β phases. Almost all β grains were twinned and strained. Symmetry-related domain structures inherited from previous high-temperature transformations were observed in β grains. Both the α→α'H and α'L →β transformations were considered to be ferroelastic, and spontaneous strains were calculated. In terms of the crystal structures, the major driving force for the β→γ transformation is proposed to be strains and cation charge repulsions in the β structure. This mechanism can be displacive, but it needs to overcome a comparatively high energy barrier. 相似文献
48.
Ian Haywood 《Cities》1985,2(3):186-197
Khartoum is the capital of a country whose creation resulted more from colonial politicians' desires for straight boundaries than from local social or political realities. This profile traces Khartoum's history of growth and planning legislation since its establishment as an administrative capital at the turn of the century. The city, developed from three separate towns, grew rapidly after independence as administrative functions grew and industrial development started. However, lack of an effective mechanism for planning and control of the city's growth has meant that a pattern of sprawling low-density land uses has developed, with inadequate services and deteriorating environmental standards. What planning control does exist operates through land-use zoning and building byelaws, though often arbitrarily applied. Planning problems are exacerbated by high population growth rates and national economic problems. 相似文献
49.
Tomik Yaghoobian Ian F. Blake 《Applicable Algebra in Engineering, Communication and Computing》1994,5(1):23-43
The subject of decoding Reed-Solomon codes is considered. By reformulating the Berlekamp and Welch key equation and introducing new versions of this key equation, two new decoding algorithms for Reed-Solomon codes will be presented. The two new decoding algorithms are significant for three reasons. Firstly the new equations and algorithms represent a novel approach to the extensively researched problem of decoding Reed-Solomon codes. Secondly the algorithms have algorithmic and implementation complexity comparable to existing decoding algorithms, and as such present a viable solution for decoding Reed-Solomon codes. Thirdly the new ideas presented suggest a direction for future research. The first algorithm uses the extended Euclidean algorithm and is very efficient for a systolic VLSI implementation. The second decoding algorithm presented is similar in nature to the original decoding algorithm of Peterson except that the syndromes do not need to be computed and the remainders are used directly. It has a regular structure and will be efficient for implementation only for correcting a small number of errors. A systolic design for computing the Lagrange interpolation of a polynomial, which is needed for the first decoding algorithm, is also presented.This research was supported by a grant from the Canadian Institute for Telecommunications Research under the NCE program of the Government of Canada 相似文献
50.