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71.
Wakui C Akiyama H Watanabe T Fitch MM Uchikawa S Ki M Takahashi K Chiba R Fujii A Hino A Maitani T 《Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan》2004,45(1):19-24
A histochemical assay for detecting genetically modified (GM) papaya (derived from Line 55-1) is described. GM papaya, currently undergoing a safety assessment in Japan, was developed using a construct that included a beta-glucuronidase (GUS) reporter gene linked to a virus coat protein (CP) gene. Histochemical assay was used to visualize the blue GUS reaction product from transgenic seed embryos. Twelve embryos per fruit were extracted from the papaya seeds using a surgical knife. The embryos were incubated with the substrate 5-bromo-4-chloro-3-indolyl-beta-D-glucuronide (X-Gluc) in a 96-well microtiter plate for 10-15 hours at 37 degrees C. Seventy-five percent of GM papaya embryos should turn blue theoretically. The histochemical assay results were completely consistent with those from a qualitative polymerase chain reaction (PCR) method developed by this laboratory. Furthermore, the method was validated in a five-laboratory study. The method for detection of GM papaya is rapid and simple, and does not require use of specialized equipment. 相似文献
72.
73.
Self-organized rhodamine 6G particles prepared by wetting/dewetting process of an ethanol solution on a hydrophilic glass surface exhibits fluorescence without quenching, showing a sharp linewidth of 2nm with a large redshift, which indicates an existence of dye aggregates, similar to J-aggregates, inside the particle. Polarized evanescent field excitation showed that the dye molecule's transition moment along the pi-conjugation was oriented unidirectionally within particles and parallel to the substrate surface. This deduced dye orientation showed correlation between adjacent, but separated, particles and pointed roughly 45 degrees off the dewetting direction. In contrast, the particles of another pi-conjugated NK1420 dye, J-aggregates of which grows easily from an oversaturated solution, showed dye orientation along the dewetting direction preferably, still indicating the effect of self-organization, however based on a different mechanism. An annealing procedure revealed that both aggregates are in quasi-stable states, which is consistent with the rapidness of the dewetting process that may lead to crystallization in nonequilibrium. 相似文献
74.
Detection of genetically modified organisms in foreign-made processed foods containing corn and potato 总被引:1,自引:0,他引:1
Monma K Araki R Sagi N Satoh M Ichikawa H Satoh K Tobe T Kamata K Hino A Saito K 《Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan》2005,46(3):79-85
Investigations of the validity of labeling regarding genetically modified (GM) products were conducted using polymerase chain reaction (PCR) methods for foreign-made processed foods made from corn and potato purchased in the Tokyo area and in the USA. Several kinds of GM crops were detected in 12 of 32 samples of processed corn samples. More than two GM events for which safety reviews have been completed in Japan were simultaneously detected in 10 samples. GM events MON810 and Bt11 were most frequently detected in the samples by qualitative PCR methods. MON810 was detected in 11 of the 12 samples, and Bt11 was detected in 6 of the 12 samples. In addition, Roundup Ready soy was detected in one of the 12 samples. On the other hand, CBH351, for which the safety assessment was withdrawn in Japan, was not detected in any of the 12 samples. A trial quantitative analysis was performed on six of the GM maize qualitatively positive samples. The estimated amounts of GM maize in these samples ranged from 0.2 to 2.8%, except for one sample, which contained 24.1%. For this sample, the total amount found by event-specific quantitative analysis was 23.8%. Additionally, Roundup Ready soy was detected in one sample of 21 potato-processed foods, although GM potatoes were not detected in any sample. 相似文献
75.
Takashi Soga Akihiro Musa Koki Okabe Kazuhiko Komatsu Ryusuke Egawa Hiroyuki Takizawa Hiroaki Kobayashi Shun Takahashi Daisuke Sasaki Kazuhiro Nakahashi 《Computers & Fluids》2011,45(1):215-221
The building-cube method (BCM) is a new generation algorithm for CFD simulations. The basic idea of BCM is to simplify the algorithm in all stages of flow computation to achieve large-scale simulations. Calculation of a pressure field using the Successive Over Relaxation (SOR) method consumes most of the total execution time required for BCM. In this paper, effective implementations on modern vector and scalar processors are investigated. NEC SX-9 and Intel Nehalem-EX are the latest vector and scalar processors. Those processors have much higher peak performances than their previous-generation processors. However, their memory bandwidth improvement cannot catch up with the performance improvement of processors. This is the so-called memory wall problem. In our paper, we discuss optimization techniques for implementation of the SOR method based on architectural characteristics of these modern processors, and evaluate their effects on the sustained performances of these processors for BCM. 相似文献
76.
Yoshiki Hoshide Yusuke Komura Akimori Tabata Akihiko Kitagawa Akihiro Kondo 《Thin solid films》2009,517(12):3520-3523
Silicon carbide (SiC) thin films were prepared by hot-wire chemical vapor deposition from SiH4/CH4/H2 and the influence of H2 gas flow rate (F(H2)) on the film properties was investigated. The SiH4 gas flow rate was 1 sccm. At the CH4 gas flow rate (F(CH4)) of 1 sccm, nanocrystalline cubic SiC (nc-3C-SiC) grew even without H2. On the other hand, at F(CH4) = 2 sccm, amorphous SiC grew without H2 and nc-3C-SiC grew above F(H2) = 50 sccm. As F(H2) was increased, the crystallinity improved both at F(CH4) = 1 and 2 sccm. However, the mean crystallite size decreased at F(CH4) = 1 sccm and increased at F(CH4) = 2 sccm. We discuss growth mechanisms of nc-3C-SiC. 相似文献
77.
Effects of Atmospheric Composition on the Molecular Structure of Synthesized Silicon Oxycarbides 下载免费PDF全文
Masaki Narisawa Fuji Funabiki Akihiro Iwase Fumihiro Wakai Hideo Hosono 《Journal of the American Ceramic Society》2015,98(10):3373-3380
The dependence of silicon oxycarbides' chemical composition and molecular structure on their reaction conditions was tested by varying the atmosphere under which pyrolysis was performed. To obtain the silicon oxycarbides, densely cross‐linked silicone resin particles with an averaged diameter of 2 μm were pyrolyzed in various atmospheres of H2, Ar, and CO2, in the temperature range 700°C–1100°C. The residual mass of resin after pyrolysis was almost constant at 700°C, although their apparent colors varied distinctly. The sample obtained from the H2 atmosphere was white, whereas that obtained from the CO2 atmosphere was dark brown. Fourier‐transform infrared (FT‐IR) spectra of the residues suggested that the Si–O–Si network evolution was accelerated in the CO2 atmosphere. Beyond 800°C, the chemical compositions of the compounds obtained from a H2 atmosphere increasingly approached near‐stoichiometric SiO2–xSiC composition with increasing the pyrolysis temperature. Compounds from a CO2 atmosphere approached a composition of SiO2–xC with no free SiC as the pyrolysis temperature increased. In the products from an Ar atmosphere, SiO2–xSiC–yC compositions were typically obtained. The observed effects of the pyrolysis atmosphere on the resulting chemical compositions were analyzed in terms of thermodynamic calculations. Electron spin resonance (ESR) spectra revealed broad and intense signals from products obtained from either Ar or CO2. Estimating from the signal intensity, the residual spin concentrations were in the range 1018–1019 g?1. Meanwhile, the spectra from the samples obtained in H2 showed weak and sharp signals with estimated spin concentrations ranging from 1016–1017 g?1. This signal attenuation may have been due to the hydrogen capping of dangling bond formed during pyrolysis. 相似文献
78.
Akihiro Ishimi Kozo Katsuyama Koji Maeda Tsuyoshi Nagamine Takeo Asaga Hirotaka Furuya 《Journal of Nuclear Science and Technology》2013,50(12):1144-1155
The X-ray CT technology previously developed by JAEA was upgraded. The shape of the X-ray source beam was changed from a circular shape to an elliptical one and the collimator slit width was decreased from 0.3 to 0.1 mm. The X-ray detector was improved by changing a CdWO4 scintillator to a highly sensitive silicon semiconductor detector. The analysis code of X-ray CT image was revised with respect to the number of points by using two kinds of experimental results and taking into account the effects of crack existence and deviation of the central void position from the radial center of a fuel pellet. As a result, high resolution X-ray CT images could be obtained on the transverse cross section of irradiated fuel assemblies. The error of the dimensional measurement was improved from ±0.1 to ±0.03 mm by upgrading the instrument and revising the analysis code of X-ray CT image. The discriminating accuracy of density difference could be increased, and the low density region (undisturbed region) and high density region (equi-axial and columnar regions) in the X-ray CT image on the cross section of irradiated fuel could be discriminated from each other. The reliability of fuel performance analysis improves because a large number of PIE data can be collected, compared with the conventional destructive PIE. 相似文献
79.
Takuro Tomita Ayahito Uetake Tsutomu Asahina Kentaro Kayanuma Akihiro Murayama Yasuo Oka 《Journal of Superconductivity and Novel Magnetism》2005,18(3):405-410
Spin injection processes in the double quantum dots of ZnSe-based diluted magnetic semiconductors are discussed. Double quantum
dots are fabricated from ZnSe-based double quantum wells by electron beam lithography and wet etching. In these samples, the
photo-excited carriers in the magnetic dots are injected into the non-magnetic dots. The circular polarization degrees of
photoluminescence from the non-magnetic dots are measured by micro-photoluminescence measurement system under the magnetic
field up to 5 T. The maximum spin polarization degrees of injected carriers determined from our experiment are 10% for double
quantum wells and 15% for double quantum dots. The spin injection efficiency was estimated both from the observed circular
polarization degree and the diffusion length of carriers. We concluded that the spin injection efficiency is increased in
the double quantum dots. 相似文献
80.
Dr. Tomoya Hirano Dr. Takashi Fujiwara Dr. Hideaki Niwa Michitake Hirano Kasumi Ohira Yusuke Okazaki Shin Sato Dr. Takashi Umehara Dr. Yuki Maemoto Prof. Akihiro Ito Prof. Minoru Yoshida Prof. Hiroyuki Kagechika 《ChemMedChem》2018,13(15):1530-1540
The histone methyltransferase SET7/9 methylates not only histone but also non‐histone proteins as substrates, and therefore, SET7/9 inhibitors are considered candidates for the treatment of diseases. Previously, our group identified cyproheptadine, used clinically as a serotonin receptor antagonist and histamine receptor (H1) antagonist, as a novel scaffold of the SET7/9 inhibitor. In this work, we focused on dibenzosuberene as a substructure of cyproheptadine and synthesized derivatives with various functional groups. Among them, the compound bearing a 2‐hydroxy group showed the most potent activity. On the other hand, a 3‐hydroxy group or another hydrophilic functional group such as acetamide decreased the activity. Structural analysis clarified a rationale for the improved potency only by tightly restricted location and type of the hydrophilic group. In addition, a SET7/9 loop, which was only partially visible in the complex with cyproheptadine, became more clearly visible in the complex with 2‐hydroxycyproheptadine. These results are expected to be helpful for further structure‐based development of SET7/9 inhibitors. 相似文献