Low molecular weight oligo-β-hydroxybutyric acids and 3-hydroxy-N-phenethyl-butyramide – new products from microorganisms

Summary A new group of low-molecular weight channel-forming oligo(hydroxybutyric acids) (cPHBs, 1 with n = 8–30; main component MW ≈ 1300 dalton) was isolated from microorganisms of different origin. Inclusion bodies were electron-microscopically visible in cells in the state of autolysis, not in cells in the exponential phase of growth. cPHB and high-molecular poly(l3-hydroxybutyric acid) (sPHB) is cleaved by phenylethylamine and forms the corresponding monomeric hydroxybutyramide and – under drastic conditions, the crotylamide. One of these compounds, the 3-hydroxy-N-phenethyl-butyramide (5), was isolated as a new natural product now. Received: 28 March 2002/Revised version: 26 July 2002/ Accepted: 26 July 2002 RID="*" ID="*"Marine Bakterien, XVII. XVI: R.P. Maskey, R.N. Asolkar, E. Helmke, and H. Laatsch, Chalcomycin B, a new antibiotic from a marine Streptomyces sp. B7064. J. Antibiot., submitted 2002 Correspondence to Hartmut Laatsch, e-mail: hlaatsc@gwdg.de, Fax: +49-551-399660     

Skills required for participating in CMC courses: An empirical study

The development of new communication technologies and their applications has opened a broad spectrum of options to promote learning, of which a significant one is CMC – Computer-Mediated Communication. Yet, students use this medium to a relatively small extent. Our premise is that the use of these technologies depends on the level of skills and expertise of the individuals using them. The purpose of this study is to determine whether taking a Computer Literacy and Applications course (CLA) before taking CMC courses has an impact on students' participation in CMC courses and on the effective use of various CMC tools. The sample consisted of 153 students enrolled in CMC courses in the Social Sciences in the Spring 2001/2 and Fall 2002/3 semesters. Participants were divided into two groups: 80 students who had taken CLA, and 73 students who had not. A questionnaire to assess the impact of CLA on participation in CMC courses was administered to the two groups. The results of the study indicate that participation in CMC courses is more intensive in the group that had taken CLA. Based on these findings, we recommend mandating a CLA course, like the one developed at the Open University of Israel (OUI), before taking CMC courses.     

OPM/Web – Object-Process Methodology for Developing Web Applications

Web applications can be classified as hybrids between hypermedia and information systems. They have a relatively simple distributed architecture from the user viewpoint, but a complex dynamic architecture from the designer viewpoint. They need to respond to operation by an unlimited number of heterogeneously skilled users, address security and privacy concerns, access heterogeneous, up-to-date information sources, and exhibit dynamic behaviors that involve such processes as code transferring. Common system development methods can model some of these aspects, but none of them is sufficient to specify the large spectrum of Web application concepts and requirements. This paper introduces OPM/Web, an extension to the Object-Process Methodology (OPM) that satisfies the functional, structural and behavioral Web-based information system requirements. The main extensions of OPM/Web are adding properties of links to express requirements, such as those related to encryption; extending the zooming and unfolding facilities to increase modularity; cleanly separating declarations and instances of code to model code transferring; and adding global data integrity and control constraints to express dependence or temporal relations among (physically) separate modules. We present a case study that helps evaluate OPM/Web and compare it to an extension of the Unified Modeling Language (UML) for the Web application domain.     

Effects of 2-(2,4-dimethylphenyl)indan-1,3-dione on serum lipoprotein and lipid metabolism of rodents

2-(2,4-Dimethylphenyl)indan-1,3-dione was shown to be a potent hypolipidemic agent in rodents, lowering significantly both serum cholesterol and triglyceride levels at 20 mg/kg/day. The agent in vivo inhibited the enzymatic activities of ATP-dependent citrate lyase, acetyl-CoA synthetase, cholesterol-7α-hydroxylase, acyl-CoA cholesterol acyl transferase,sn-glycerol-3-phosphate acyl transferase and phosphatidylate phosphohydrolase. Tissue lipid levels of liver and small intestine also were reduced by the agent. The rat serum lipoprotein lipid content was modulated by the drug, which should be favorable for the removable of cholesterol from peripheral tissue for conduction to the liver for clearance from the body. Low density lipoprotein (LDL) cholesterol levels were reduced after treatment, which suggests that the agent potentially reduces deposition of cholesterol in plaques. If chemotherapy for atherosclerosis is to be successful, then the high density lipoprotein (HDL) cholesterol level needs to be elevated more than 16% to 25%, the level produced by current hypolipidemic agents. 2-(2,4-Dimethylphenyl)-indan-1,3-dione offers a 75% increase in HDL cholesterol levels and a 30% reduction of LDL cholesterol levels with a suppression of de novo synthesis of lipids and a reduction of tissue cholesterol deposition.     

Optical approach for determining strain anisotropy in quantum wells

Anisotropic in-plane strain arises in quantum-well systems by design or unintentionally. We propose two methods of measuring the in-plane strain anisotropy based on the optical polarization anisotropy that arises with anisotropic in-plane strain. One method uses purely optical means to determine the strain anisotropy in quantum wells under a compressive strain that is spatially varying. A second approach, applicable to quantum wells under tensile strain or with strain that does not vary with position, requires the application of a uniaxial in-plane stress. Although the second method is experimentally more difficult, it allows analysis of systems that would otherwise be inaccessible.     

Predator odor-induced conditioned fear involves the basolateral and medial amygdala.

The basolateral (BLA) and medial nucleus (MeA) of the amygdala participate in the modulation of unconditioned fear induced by predator odor. However, the specific role of these amygdalar nuclei in predator odor-induced fear memory is not known. Therefore, fiber-sparing lesions or temporary inactivation of the BLA or MeA were made either prior to or after exposure to cat odor, and conditioned contextual fear behavior was examined the next day. BLA and MeA lesions produced significant reductions in cat odor-induced unconditioned and conditioned fear-related behavior. In addition, temporary pharmacological neural inactivation methods occurring after exposure to cat odor revealed subtle behavioral alterations indicative of a role of the BLA in fear memory consolidation but not memory retrieval. In contrast, the MeA appears to play a specific role in retrieval but not consolidation. Results show that the BLA participates in the conditioned and unconditioned cat odor stimulus association that underlies fear memory, underscore a novel role of the MeA in predator odor contextual conditioning, and demonstrate different roles of the BLA and MeA in modulating consolidation and retrieval of predator odor fear memory. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

On the Effective Deployment of Functional Formal Verification

We examine IBM's exploitation of formal verification using RuleBase—a formal verification tool developed by the IBM Haifa Research Laboratory. The goal of the paper is methodological. We identify an integrated methodology for the deployment of formal verification which involves three complementary modes: architectural verification, block-level verification, and design exploration.     

DIABLOG: a simulation program of insulin-glucose dynamics for education of diabetics

This paper presents a dialogue computer program, DIABLOG, for the education of diabetic patients with insulin therapy. Through mathematical modelling of glucose-insulin dynamics this program is able to simulate glucose and insulin profiles of a 24 h period and display them graphically as curves. The subjects could vary the carbohydrate content of meals, the injection time and dose of short-acting and intermediate-acting insulin and could switch to insulin pump therapy. For a first evaluation 22 patients with insulin-dependent diabetes mellitus tested the program and their comments were recorded by a questionnaire. The results indicate a good acceptance of the program even by patients with no previous computer experience. Further suggestions by the patients will be discussed as well.     

Microstructure and Compression Strength of Novel TRIP‐Steel/Mg‐PSZ Composites

Novel composites on basis of austenitic stainless TRIP‐steel as matrix with reinforcements of Mg‐PSZ are presented. Compact rods were produced by cold isostatic pressing and sintering, square honeycomb samples by the ceramic extrusion technique. The samples are characterized by optical and scanning electron microscopy before and after deformation, showing the microstructure and the deformation‐ induced martensite formation. The mechanical properties of samples with 5 vol% zirconia are superior compared to zirconia‐free samples and composites with higher zirconia contents in terms of bending and compression tests. The honeycomb samples exhibit extraordinary high specific energy absorption in compression.