Simple and comprehensive two-dimensional reversed-phase HPLC using monolithic silica columns

Simple and comprehensive two-dimensional (2D)-HPLC was studied in a reversed-phase mode using monolithic silica columns for second-dimension (2nd-D) separation. Every fraction from the first column, 15 cm long (4.6-mm i.d.), packed with fluoroalkylsilyl-bonded (FR) silica particles, was subjected to the separation in the 2nd-D using one or two octadecylsilylated (C(18)) monolithic silica columns (4.6-mm i.d., 3 cm). Monolithic silica columns in the 2nd-D were eluted at a flow rate of up to 10 mL/min with separation time of 30 s that meets the fractionation every 15-30 s at the first dimension (1st-D) operated at a flow rate of 0.4-0.8 mL/min. Three cases were studied. (1) In the simplest scheme of 2D-HPLC, effluent of the 1st-D was directly loaded into an injector loop of 2nd-D HPLC for 28 s, and 2 s was allowed for injection. (2) Two six-port valves each having a sample loop were used to hold the effluent of the 1st-D alternately for 30 s for one 2nd-D column to effect comprehensive 2D-HPLC without the loss of 1st-D effluent. (3) Two monolithic silica columns were used for 2nd-D by using a switching valve and two sets of 2nd-D chromatographs separating each fraction of the 1st-D effluent with the two 2nd-D columns alternately. In this case, two columns of the same stationary phase (C(18)) or different phases, C(18) and (pentabromobenzyloxy)propylsilyl-bonded (PBB), could be employed at the 2nd-D, although the latter needed two complementary runs. The systems produced peak capacity of approximately 1000 in approximately 60 min in cases 1 and 2 and in approximately 30 min in case 3. The three stationary phases, FR, C(18), and PBB, showed widely different selectivity from each other, making 2D separations possible. The simple and comprehensive 2D-HPLC utilizes the stability and high efficiency at high linear velocities of monolithic silica columns.     

On constructing communication protocols from component-based service specifications

Constructing communication protocols from component service specifications, each of which specifies a subfunction of the target protocol, enables efficient development of a large and complex communication protocol. Concerning this construction, related techniques have been already proposed: integration of component protocol specifications into a single protocol specification and transformation of service specifications to protocol specifications. However, the integration needs special knowledge of communication protocols, and the transformation requires that a large and complex service specification should be developed as input to produce the target protocol. In order to cope with these problems, this paper proposes a new method which at first integrates component service specifications into a single service specification, and then transforms the service specification into the target protocol by a protocol synthesis technique. The most important point of view is that component integration is performed at the service specification level rather than the protocol specification level. Additionally, we define a class of ‘well-formed’ service specification which ensures correctness of the target protocol. As a result, the integration and transformation can be efficiently executed in small state space without special knowledge of communication protocols. Finally, we have shown the effectiveness of the proposed method by constructing a part of the real-life OSI protocol FTAM.     

Mean–variance relationship of the number of flows in traffic aggregation and its application to traffic management

We consider the mean–variance relationship of the number of flows in traffic aggregation, where flows are divided into several groups randomly, based on a predefined flow aggregation index, such as source IP address. We first derive a quadratic relationship between the mean and the variance of the number of flows belonging to a randomly chosen traffic aggregation group. Note here that the result is applicable to sampled flows obtained through packet sampling. We then show that our analytically derived mean–variance relationship fits well those in actual packet trace data sets. Next, we present two applications of the mean–variance relationship to traffic management. One is an application to detecting network anomalies through monitoring a time series of traffic. Using the mean–variance relationship, we determine the traffic aggregation level in traffic monitoring so that it meets two predefined requirements on false positive and false negative ratios simultaneously. The other is an application to load balancing among network equipments that require per-flow management. We utilize the mean–variance relationship for estimating the processing capability required in each network equipment.     

Nonlinear repetitive control with application to trajectory control of manipulators

In this article, a new control scheme named repetitive control is proposed for a class of nonlinear systems described by x(t) = Ax(t) + Bu(t) + n(x(t)) and y(t) = Cx(t), in which the controlled variables follow periodic reference commands. The stability condition is derived by applying the passivity theorem. We show how to apply the repetitive control scheme to the trajectory control of a manipulator. A simple repetitive control scheme is developed for the trajectory control of a manipulator by using nonlinear compensation and feedbacks of position and velocity signals. Experimental results for a three link manipulator verify that the proposed repetitive control reduces the tracking error to a very low level.     

Switched capacitor circuit for 1/f noise generation using a power-law circuit

A simple switched capacitor (SC) circuit is given which realizes a one-dimensional discrete dynamical system X_n+1 = X_n + ux^z generating intermittent chaos with a 1/f-shaped power spectrum. an integrated circuit with a pair of logarithmic and antilogarithmic amplifiters can easily realize the power-law term x^z which plays a key role in generating l/fnoise. an inevitable small offset voltage ? in the SC circuit governs the cut-off frequency of 1/f noise, and when the dynamical system is modified by introducing an effective ?, the ‘indirect method’ enables us to get the precise power spectrum in the modified system.     

Species specificity of musty odor producing Phormidium tenue in Lake Kamafusa.

16S ribosomal DNA sequences of various strains of Phormidium tenue (P. tenue) in Lake Kamafusa were determined. Such P. tenue could not be distinguished under microscopic examination. From the results of the analyses of base sequences, and using a phylogenetic tree, several P. tenue with different species specificity on the sequence were found in the lake. P. tenue were divided into three groups. The first produced 2-methylisoborneol (MIB) and was close to a species from the moat of Nagoya castle. The second produced MIB and was characteristic of Lake Kamafusa. The third had a brown color like a Lake Biwa strain and was MIB non-producing. From the phylogenetic tree, it was revealed that species had not changed recently by mutation. Moreover, a detection method for P. tenue by PCR and dot hybridization was established.