Effects of water velocity and turbulence on the behaviour of Iberian barbel (Luciobarbus bocagei,Steindachner 1864) in an experimental pool‐type fishway

The restoration of fish passage has been focused on anadromous fish species, whilst studies accommodating passage of coarse species have often been considered incidental, yet frequently these are the predominant group of species encountered in rivers. In addition, fishway designs depend greatly on the interplay between hydraulics and biomechanics, yet very little data are available on the responses to specific hydraulic settings for these species. This study aims to explore the effects of water velocity and turbulence on the behaviour of a cyprinid species ‐ the Iberian barbel Luciobarbus bocagei (Steindachner, 1864) ‐ particularly their upstream movements upon different discharges (38.5 to 77.0 L · s^?1), through an indoor full scale pool‐type fishway prototype. Larger adults had a higher passage success (mean = 79%) and took less time (mean ± SD (min): 5.7 ± 1.3) to negotiate the entire six pool fishway, when compared to small adults. Correlation analysis between hydraulic variables and fish transit time yielded different results. Correlations were found to be the highest between the horizontal component of Reynolds shear stress and fish transit time, particularly for smaller size‐individuals (r = ?0.45; p < 0.001), highlighting this variable as a key‐parameter which strongly determines the movements of Iberian barbel. The present study identified key factors on Iberian barbel movements that may have direct application to future fishway designs for this species and for other ‘weak’ swimmers. Copyright © 2010 John Wiley & Sons, Ltd.     

Real-time Stabilization of a Quadrotor UAV: Nonlinear Optimal and Suboptimal Control

In this paper a nonlinear suboptimal stabilizing control strategy based on Control Lyapunov Functions (CLF) is synthesized and applied to a quadrotor helicopter. Sufficient conditions are obtained for this control law to ensure the asymptotic stability of the closed loop system. Furthermore, a particular methodology to find a CLF candidate for nonlinear affine system is also presented, which is highly relevant because the dynamical model representing the VTOL aerial vehicles have this affine structure. Using this CLF candidate, we are able to synthesize a nonlinear stabilizing optimal control law which allows energy saving. Numerical simulations were developed for both control strategies and real time experiments have been performed using the nonlinear stabilizing control algorithm. The numerical simulations have shown a successful performance of the autonomous aerial vehicle.     

Research opportunities in information technology funding and system justification

This article reviews IT funding and system justification practice and research in two dimensions, previews papers in the special issue on ‘Information technology funding and system justification in the organization’, and identifies opportunities for research in IT funding. IT funding decisions have been hard problems for firms and, because IT investments are so pervasive, they have been very important. Here we review IT funding decisions and research about IT funding historically, using two dimensions, justification dynamism and justification evidence. Over time, the IT funding decisions have changed from static, one time events to iterative and even continuous efforts. Early IT funding decisions were based on finance and accounting models, but changes in the purposes of new systems have necessitated justification based on a variety of qualitative measures. We preview the six papers in the special issue, with an eye to introducing them to readers and also to looking for areas that represent opportunites for future IS research. Finally, we identify eight areas that should represent good opportunities for future research in IT funding and systems justification.     

MRT letter: Extended depth from focus reconstruction method for stretch zone measurement in 15-5PH steel

The stretch zone width (SZW) data for 15‐5PH steel CTOD specimens fractured at ?150°C to + 23°C temperature were measured based on focused images and 3D maps obtained by extended depth‐of‐field reconstruction from light microscopy (LM) image stacks. This LM‐based method, with a larger lateral resolution, seems to be as effective for quantitative analysis of SZW as scanning electron microscopy (SEM) or confocal scanning laser microscopy (CSLM), permitting to clearly identify stretch zone boundaries. Despite the worst sharpness of focused images, a robust linear correlation was established to fracture toughness (K_C) and SZW data for the 15‐5PH steel tested specimens, measured at their center region. The method is an alternative to evaluate the boundaries of stretched zones, at a lower cost of implementation and training, since topographic data from elevation maps can be associated with reconstructed image, which summarizes the original contrast and brightness information. Finally, the extended depth‐of‐field method is presented here as a valuable tool for failure analysis, as a cheaper alternative to investigate rough surfaces or fracture, compared to scanning electron or confocal light microscopes. Microsc. Res. Tech. 75:1155–1158, 2012. © 2012 Wiley Periodicals, Inc.     

Evaluation of total mercury concentrations in fish consumed in the municipality of Itaituba, Tapajós River Basin, Pará, Brazil

The environmental exposure to mercury by riverine and Indian communities can occur through the ingestion of fish and products contaminated by mercury compounds. The present study aims to evaluate the total mercury concentration in the different fish species most consumed in the municipality of Itaituba, Tapajós river basin, where there are intense gold-mining activities. These fish samples were analyzed by atomic absorption spectrophotometry by cold vapor technique, Automatic Mercury Analyzer HG-3500. The largest mercury concentrations were found in the carnivorous species ranging from 112.4 to 2250 microg/g, while the detritivorous, herbivorous and omnivorous species presented total mercury levels ranging from 3.2 to 309.8 microg/g, which is below the limit established by the World Health Organization. This paper also reports and identifies which species are more polluted and present a statistical relationship among concentration and weight of the carnivorous species studied in detail, Brachyplatystoma flavicans (dourada) (r2 = 0.691) and Pseudoplatystoma sp. (surubim) (r2 = 0.654).     

Physical properties and biocompatibility of chitosan/soy blended membranes

Blends of polysaccharides and proteins are a source for the development of novel materials with interesting and tailorable properties, with potential to be used in a range of biomedical applications. in this work a series of blended membranes composed by chitosan and soy protein isolate was prepared by solvent casting methodology. in addition, cross-linking was performed in situ with glutaraldehyde solutions in the range 5 × 10^–3 – 0.1 M. Furthermore, the influence of the composition and cross-linking on the degradation behaviour, water uptake and cell adhesion was investigated. The obtained results showed that the incorporation of chitosan, associated to network formation by cross linking, promoted a slight decrease of water absorption and a slower degradability of the membranes. Moreover, direct contact biocompatibility studies, with L929 cells, indicate that the cross-linking enhances the capability of the material to support cell growth.     

Radio-Frequency Self-Referencing Technique With Enhanced Sensitivity for Coarse WDM Fiber Optic Intensity Sensors

A self-referenced passive optical network (PON) with coarse wavelength division multiplexing (CWDM) is reported for remotely addressing optical intensity sensors with enhanced sensitivity. The self-referencing remote configuration is described as a finite-impulse-response (FIR) filter in reflective operation using two fiber Bragg gratings (FBG) and a fiber delay line. The antisymmetrical phase response of the configuration permits to achieve a self-referencing measurement parameter two times more sensitive than reported previously. To enhance the power budget of the network, CWDM devices with low insertion losses are used for spectral splitting of a radio-frequency (RF) modulated broadband light source (BLS). The network topology and the sensor's interrogation technique are theoretically analyzed and experimental results validating the models are reported.     

Zinc Prevents DNA Damage in Normal Cells but Shows Genotoxic and Cytotoxic Effects in Acute Myeloid Leukemia Cells

Genomic instability is prevented by the DNA damage response (DDR). Micronutrients, like zinc (Zn), are cofactors of DDR proteins, and micronutrient deficiencies have been related to increased cancer risk. Acute myeloid leukemia (AML) patients commonly present Zn deficiency. Moreover, reports point to DDR defects in AML. We studied the effects of Zn in DDR modulation in AML. Cell lines of AML (HEL) and normal human lymphocytes (IMC) were cultured in standard culture, Zn depletion, and supplementation (40 μM ZnSO₄) conditions and exposed to hydrogen peroxide (H₂O₂) or ultraviolet (UV) radiation. Chromosomal damage, cell death, and nuclear division indexes (NDI) were assessed through cytokinesis-block micronucleus assay. The phosphorylated histone H2AX (yH2AX) expression was monitored at 0 h, 1 h, and 24 h after exposure. Expression of DDR genes was evaluated by quantitative real time polymerase chain reaction (qPCR). Zn supplementation increased the genotoxicity of H₂O₂ and UV radiation in AML cells, induced cytotoxic and antiproliferative effects, and led to persistent yH2AX activation. In contrast, in normal lymphocytes, supplementation decreased damage rates, while Zn depletion favored damage accumulation and impaired repair kinetics. Gene expression was not affected by Zn depletion or supplementation. Zn presented a dual role in the modulation of genome damage, preventing damage accumulation in normal cells and increasing genotoxicity and cytotoxicity in AML cells.     

Flexible three-dimensional modeling of plants using low- resolution cameras and visual odometry

The three-dimensional reconstruction of plants using computer vision methods is a promising alternative to non-destructive metrology in plant phenotyping. However, diversity in plants form and size, different surrounding environments (laboratory, greenhouse or field), and occlusions impose challenging issues. We propose the use of state-of-the-art methods for visual odometry to accurately recover camera pose and preliminary three-dimensional models on image acquisition time. Specimens of maize and sunflower were imaged using a single free-moving camera and a software tool with visual odometry capabilities. Multiple-view stereo was employed to produce dense point clouds sampling the plant surfaces. The produced three-dimensional models are accurate snapshots of the shoot state and plant measurements can be recovered in a non-invasive way. The results show a free-moving low-resolution camera is able to handle occlusions and variations in plant size and form, allowing the reconstruction of different species, and specimens in different stages of development. It is also a cheap and flexible method, suitable for different phenotyping needs. Plant traits were computed from the point clouds and compared to manually measured reference, showing millimeter accuracy. All data, including images, camera calibration, pose, and three-dimensional models are publicly available.