Aerobic biodegradation of di-n-butyl phthalate by Xiangjiang River sediment and microflora analysis

Di-n-butyl phthalate (DBP), one of phthalate acid esters (PAEs), was investigated to determine its biodegradation rate using Xiangjiang River sediment and find potential DBP degraders in the enrichment culture of the sediment. The sediment sample was incubated with an initial concentration of DBP of 100 mg/L for 5 d. The biodegradation rate of DBP was detected using HPLC and the degraded products were analyzed by GC/MS. Subsequently, the microbial diversity of the enrichment culture was analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The results reveal that almost 100% of DBP is degraded after merely 3 d, generating two main degraded products: mono-butyl phthalate (MBP) and 9-octadecenoic acid. After a six-month enrichment period under the pressure of DBP, the dominant family in the final enrichment culture is clustered with the Comamonas sp., the remaining are affiliated with Sphingomonas sp., Hydrogenophaga sp., Rhizobium sp., and Acidovorax sp. The results show the potential of these bacteria to be used in the bioremediation of DBP in the environment.     

Identification and fermentation optimization of protopectinase-overproducing strain Aspergillus niger CD-01 for pectin production

In order to solve the citrus peel resource waste problem and minimize the drawbacks of chemical extraction of pectin, a protopectinase-overproducing strain CD-01 for pectin production was isolated from a pit soil dumped with perished orange in Changde City, Hunan Province of China. The strain CD-01 had the same morphology and 28S rRNA gene sequence (FJ184995) as that of Aspergillus niger (ATCC 64028). It was thus identified and named as Aspergillus niger CD-01. The fermentation condition was optimized based on L₉(3⁴) orthogonal experimental design and the variances analyses. The results show that the optimal condition for producing pectin is as follows: time 36 h, temperature 35 °C, pH 5, and urea as the nitrogen source. Under this condition, the pectin yield can reach up to 24.5%. This shows a great potential of Aspergillus niger CD-01 in pectin extraction from citrus. Foundation item: Projects(50621063, 50674101) supported by the National Natural Science Foundation of China     

高效产表面活性剂菌株（Lz2～1）的筛选及其特性研究

从24份含油土壤和水等样品中,经富集培养、摇瓶培养和排油活性测定等方法筛选出一株能以原油为碳源产生表面活性剂的菌株Lz2—1;该菌株可以将水的表面张力由72mN／m降到28mN／m,且发酵液具有较好的乳化活性;经生理生化16SrDNA及生理生化实验确定该菌株为铜绿假单胞菌;提取其代谢产物经薄层层析及红外色谱分析显示,主要的表面活性剂类物质为鼠李糖脂类,其临界胶束质量浓度为0．63047g／L。结果表明,表面活性物质是Lz2—1菌株在微生物采油过程中发挥作用的主要因素。     

HIV-1蛋白酶在大肠杆菌中的表达

分泌融合表达HIV-1蛋白酶(Protease,PR),用于对HIV蛋白酶抑制剂的筛选.利用重叠延伸PCR方法,获得使用大肠杆菌偏爱密码子的编码HIV-1蛋白酶(PR)的基因,将目的基因重组至原核表达载体pET-SP-DsbA-T,得到"pET-SP-DsbA-PRsite-PR"分泌融合表达型载体,重组载体转化大肠杆菌BL21(DE3).工程菌在LB培养基中添加终浓度为0.1 mM的IPTG诱导表达,SDS-PAGE分析周质蛋白.DNA测序结果证实合成的HIV-1 PR基因与设计的序列完全一致,在周质空间表达出具有自切割活性的HIV-1 PR蛋白.研究成功合成并克隆了使用大肠杆菌偏好密码子的HIV-1 PR的DNA编码序列,经表达鉴定得到具有自切割活性的HIV-1 PR蛋白.     

用Fenton试剂-矿化垃圾生物反应器联合处理离子交换树脂再生废水

研究了Fenton试剂-矿化垃圾生物反应器联合处理离子交换树脂再生废水.研究结果表明：当原水CODCr为413 mg/L和过氧化氢投加量为24 mL/L（废水）时,经Fenton试剂预处理后,再经矿化垃圾生物反应器后续生化处理（运行周期为4 h,1 h进水,3 h落干,进水流量为600 mL/h）,出水CODCr降至85 mg/L左右,达到国家排放标准.     

基于PSS/EBKDY模块的短路电流周期分量计算方法分析

介绍了一种基于电网仿真软件PSS／EBKDY模块的短路电流周期分量计算方法,分别用PSS／E和BPA机电暂态仿真软件对计算结果进行了校核．仿真结果显示,基于PSS／EBKDY模块的短路电流算法有较好的精确度,但该算法的缺点是不够保守,这将影响其在工程短路电流计算上的应用．     

J2EE Web服务和.Net Web服务的集成

研究了在J2EE和．Net环境下Web服务的开发方法、J2EE调用、．NetWeb服务的运行机制及使用．NetWsdl文档创建存根的方法,实现了J2EE和．Net环境下web服务的跨平台互操作．     

含φ-伪压缩映射的迭代序列等价性问题研究

在一般的Banach空间中,对不要求Lipschitz连续的φ-伪压缩及φ-增生映射讨论了具误差的Mann迭代与Ishikawa迭代算法的等价性.     

NO在HZSM-5分子筛中吸附的实验与分子模拟

通过实验测定了NO在HZSM-5型中部分条件下的吸附性能,然后运用巨正则系综Monte Carlo方法（grand canonical assemblage Monte Carlo,GCMC）建立了相应的吸附模型,模拟计算了其相应的吸附行为．在实验与模拟结果相吻合的基础上,进一步模拟计算了NO在HZSM-5型分子筛中的吸附等温线、吸附微观构型及吸附热等性质．计算结果表明,随着硅铝比及分子筛中的自由体积增大,其对NO的吸附量增加,NO主要吸附在十元环直孔道和Zigzag形状的十元环孔道的中央;氢离子的存在在一定程度上阻碍了NO的扩散;在吸附量相同的条件下,NO在HZSM-5型分子筛的吸附热随着硅铝比的升高而升高．