Study of growth dislocations in L-arginine phosphate monohydrate single crystals by chemical etching

The results of a study of the distribution of growth dislocations revealed on different faces of L-arginine phosphate monohydrate (LAP) crystals by selective etching in relation to the growth conditions of the crystals are described. It was found that (1) the dislocation density, , on a face is the highest in its central regions and depends on the supersaturation used for growth, and that (2) on different faces is different. The observations are discussed from the standpoint of the mechanism of generation of dislocations at the seed-crystal interface in the initial stages of regeneration of the seed and at the crystal-medium interface due to the formation of bunches of growth layers on the growing crystal faces during their development.     

Structural organization of the major autolysin from Streptococcus pneumoniae

LytA amidase is the best known bacterial autolysin. It breaks down the N-acetylmuramoyl-L-alanine bonds in the peptidoglycan backbone of Streptococcus pneumoniae and requires the presence of choline residues in the cell-wall teichoic acids for activity. Genetic experiments have supported the hypothesis that its 36-kDa chain has evolved by the fusion of two independent modules: the NH2-terminal module, responsible for the catalytic activity, and the COOH-terminal module, involved in the attachment to the cell wall. The structural organization of LytA amidase and of its isolated COOH-terminal module (C-LytA) and the variations induced by choline binding have been examined by differential scanning calorimetry and analytical ultracentrifugation. Deconvolution of calorimetric curves have revealed a folding of the polypeptide chain in several independent or quasi-independent cooperative domains. Elementary transitions in C-LytA are close but not identical to those assigned to the COOH-terminal module in the complete amidase, particularly in the absence of choline. These results indicate that the NH2-terminal region of the protein is important for attaining the native tertiary fold of the COOH terminus. Analytical ultracentrifugation studies have shown that LytA exhibits a monomer <--> dimer association equilibrium, through the COOH-terminal part of the molecule. Dimerization is regulated by choline interaction and involves the preferential binding of two molecules of choline per dimer. Sedimentation velocity experiments give frictional ratios of 1.1 for C-LytA monomer and 1.4 for C-LytA and LytA dimers; values that deviated from that of globular rigid particles. When considered together, present results give evidence that LytA amidase might be described as an elongated molecule consisting of at least four domains per subunit (two per module) designated here in as N1, N2, C1, and C2. Intersubunit cooperative interactions through the C2 domain in LytA dimer occur under all experimental conditions, while C-LytA requires the saturation of low affinity choline binding sites. The relevance of the structural features deduced here for LytA amidase is examined in connection with its biological function.     

Factors causing stress in patients in intensive care units

Intensive care units have been considered stress generating areas. Knowing the causes why this happens will allow us to take specific measures to prevent or minimize it. This study has been performed with the aim to identify stress raising factors, as they are perceived by intensive care patients. The study has been performed in 49 patients most of whom were being attended in postoperatory control. The valuation of the degree of stress was performed using the "Scale of Environmental Stressors in Intensive Care" by Ballard in 1981, modified and adapted to our environment, with a result of 43 items distributed in six groups; Immobilization, Isolation, Deprivation of sleep, Time-spacial disorientation, Sensorial deprivation and overestimulation, and depersonalization and loss of autocontrol. The level of stress perceived by patients was low. The factors considered as most stressing were those related to physical aspects; presence of tubes in nose and mouth, impossibility to sleep and presence of noise, whereas those less stressing referred to Nursing attention. We conclude that patients perceive ICU as a little stressing place in spite of the excessive noise, remark the presence of invasive tubes and the difficulty to sleep as the most stressing factors, and in the same way, express a high degree of satisfaction about the attention received.     

Diagnostic value of nasal provocation testing and rhinomanometry in allergic rhinitis

Assessment of the hemodynamic and anatomic results following balloon angioplasty of discrete native coarctation of the aorta, with particular attention to "remodeling," has required repeat cardiac catheterization and angiography, which is invasive and has limited resolution. Eight patients with hypertension and discrete native coarctation with an otherwise normally developed aortic arch underwent angioplasty at 5.0 +/- 6.8 years of age. Angiographic cross-sectional areas of the aorta indexed to body surface area at the isthmus (I), coarctation site (C), and 1 cm distal to the coarctation site (Cd) pre- and postangioplasty were compared with MRI-indexed cross-sectional areas 18 +/- 10 months (MRI-1) and 35 +/- 11 months (MRI-2) postangioplasty. From preangioplasty to MRI-2, the isthmus was smaller (149 +/- 22 versus 127 +/- 27 mm2/m2; p < 0. 05). The coarctation site was larger postangioplasty (25 +/- 9 versus 116 +/- 40 mm2/m2; p < 0.001) with continued growth at latest follow-up (116 +/- 40 versus 164 +/- 36 mm2/m2; p < 0.01). The segment 1 cm distal to the coarctation site continued to decrease in area at latest follow-up (267 +/- 78 versus 163 +/- 38 mm2/m2; p < 0. 001). I versus C versus Cd at MRI-2 were similar, whereas postangioplasty and MRI-1 cross-sectional area measurements were significantly different. Following angioplasty of discrete native coarctation, the aorta becomes more uniform or undergoes "remodeling." Noninvasive MRI is an effective means of evaluating the anatomic result following balloon angioplasty, obviating the need for repeated invasive cardiac catheterizations.     

Therapeutic strategies in acute myocardial infarction. Results of STIM 93 registry

A registry was set up by the national college of cardiologists practicing in general hospitals in February 1993. The data concerned mode of admission, demographic details, initial clinical and haemodynamic evaluation and hospital outcome. Special attention was given to the electrocardiographic changes before and, in patients receiving thrombolytic therapy, after treatment. An analysis of predictive factors for mortality was performed both in the group of patients "revascularised" and in the group treated conventionally. One thousand and twenty three cases from 327 centres were analysed. There were 1292 men and 531 women, with an average age of 67.9 years. The average time interval from onset of symptoms to hospital admission was 5 h 30 min, 56.8% of patients arriving within 6 hours. 36.4% of the population underwent thrombolysis or angioplasty, 75% of patients under 75 years of age admitted before the 5th hours underwent a procedure of myocardial revascularisation. The hospital mortality was 14%, 8.7% in those revascularised and 17% in patients treated conventionally. Factors predictive of mortality were age, female gender, Killip Classes III or IV, systolic blood pressure of less than 100 mmHg, heart rate of more than 100/min and contraindications of thrombolysis. The maximum ST depression, the sum of ST depression, the sum of ST elevation and depression, were also significant predictive factors of a fatal hospital outcome in the whole population group and in patients treated conventionally. In the reperfused group, only the initial sum of ST elevation and depression was predictive of mortality: 120 minutes after the beginning of thrombolysis, the sum of ST elevations and of elevations and depressions was predictive of twice the mortality when the values exceeded 0.6 mv and 1.4 mv respectively.     

Drawings of graphs on surfaces with few crossings

We give drawings of a complete graphK _n withO(n ⁴ log² g/g) many crossings on an orientable or nonorientable surface of genusg 2. We use these drawings ofK _n and give a polynomial-time algorithm for drawing any graph withn vertices andm edges withO(m ² log² g/g) many crossings on an orientable or nonorientable surface of genusg 2. Moreover, we derive lower bounds on the crossing number of any graph on a surface of genusg 0. The number of crossings in the drawings produced by our algorithm are within a multiplicative factor ofO(log² g) from the lower bound (and hence from the optimal) for any graph withm 8n andn ²/m g m/64.The research of the third and the fourth authors was partially supported by Grant No. 2/1138/94 of the Slovak Academy of Sciences and by EC Cooperative action IC1000 Algorithms for Future Technologies (Project ALTEC). A preliminary version of this paper was presented at WG93 and published in Lecture Notes in Computer Science, Vol. 790, 1993, pp. 388–396.     

Yearly stepwise increments of the growth hormone dose results in a better growth response after four years in girls with Turner syndrome. Dutch Working Group on Growth Hormone

To optimize the growth promoting effect of growth hormone (GH), 65 previously untreated girls with Turner syndrome (TS), chronological age (CA) 2-11 yr, were randomized into 3 dosage regimen groups: A, B, and C, with a daily recombinant-human GH dose during 4 study years of 4-4-4-4, 4-6-6-6, and 4-6-8-8 IU/m2 b.s. The first GH dosage increase in groups B and C resulted in a significantly higher mean height velocity (HV) compared with constant dose group A. During the third year, when the dose was raised again only in group C, mean HV was significantly higher in groups B and C than in group A, and in group C compared with group B. In year 4 only group C mean HV remained significantly higher than group A. The pattern of change in HSDSCA (Dutch-Swedish-Danish Turner references) was identical; however, in year 4 mean delta HSDSCA in group B also remained significantly higher than group A. After 4 yr GH treatment, the following was determined. 1) The mean delta HSDSCA was significantly higher for groups B and C compared with group A, but not significantly different between groups B and C. 2) Although significantly higher compared with estimated values for untreated Dutch girls with TS, bone maturation of the GH treated girls was not significantly different between groups. 3) It was positively related with the degree of bone age (BA) retardation at start of study and negatively with baseline CA. 4) Both the modified Index of Potential Height (mIPHRUS) and a recently developed Turner-specific final height (FH) prediction method (PTSRUS), based on regression coefficients for H, CA, and bone age, showed significant increases in mean FH prediction, without significant differences between groups. PTSRUS values were markedly higher than the mIPHRUS values. Dose dependency could be shown for the area under the curve (AUC) for GH, but delta HSDSCA was not linearly related with AUC. Baseline GH binding protein (BP) levels were in 84% of the cases within the normal age range; the decrease in mean levels after 6 months GH was not significant. Mean insulin-like growth factor I (IGF-I) and IGFBP-3 plasma levels increased significantly, without significant differences between groups. delta HSDSCA during GH was dependent on IGF-I plasma levels at baseline and during the study period, beta-0.002 and beta-0.0004. Thus, a stepwise GH-dosing approach reduced the "waning" effect of the growth response after 4 yr treatment without undue bone maturation. FH prediction was not significantly different between treatment groups. Irrespective of the GH dose used, initiation of GH treatment at a younger age was beneficial after 4 yr GH when expressed as actual cm gained or as gain in FH prediction, but was not statistically significant when expressed as delta HSDSCA over the study period.     

Computations on one-dimensional cellular automata

Cellular automata may be viewed as a modelization of synchronous parallel computation. Even in the one-dimensional case, they are known as capable of universal computations. The usual proof uses a simulation of a universal Turing machine. In this paper, we present how a one-dimensional cellular automata can simulate any recursive function in such a way that composition of computations occurs as soon as possible. In addition, this allows us to show that one-dimensional cellular automata may simulate asynchronous computations.This work was supported by the Programme de Recherches Coordonnées Mathématiques et Informatique and the Esprit Basic Research Action Algebraic and Syntactical Methods in Computer Science.     

Interprocedural Array Region Analyses

Many program optimizations require exact knowledge of the sets of array elements that are referenced in or that flow between statements or procedures. Some examples are array privatization, generation of communications in distributed memory machines, or compile-time optimization of cache behavior in hierarchical memory machines. Exact array region analysis is introduced in this article. These regions exactly represent the effects of statements and procedures upon array variables. To represent the flow of these data, we also introduce two new types of array region analyses: in and out regions. The intraprocedural propagation is presented, as well as a general linear framework for interprocedural analyses, which handles array reshapes. The intra- and inter-procedural propagation of array regions is implemented in pips, the interprocedural parallelizer of fortran programs developed at École des mines de Paris.     

A semantics of introspection in a reflective prototype-based language

In Malenfant et al. [19], we have described a reflective model for a prototype-based language based on thelookup o apply reflective introspection protocol. In this paper, we augment our previous protocol by converting it to handle continuations reified as first-class objects. First-class continuations provide much more control over the current computation; during the introspection phase fired by message sending, they make it possible not only to change the behavior of the program for that message but also for the entire future computation. In this paper, we provide this introspection protocol with a formal semantics. This result is obtained by exhibiting a mapping from program configurations to priority rewrite systems (PRS) as well as a mapping from message expressions to ground first-order terms used to query the PRS. Other advantages of this approach are: to ensure the termination of the introspection using the smallest set of formally justified conditions and to provide a clear declarative account of this reflective protocol. The PRS also appears as a meta-level to the base language, independent of the implementation, but from which we derive fundamental clues to obtain an efficient language processor. By our new model, we finally highlight the link between reflection in object-oriented languages and the one originally proposed by 3-Lisp [24], although object-orientation provides reusability to reflection, making it easier to use.