Removal of N-terminal peptides from β-lactoglobulin by proteolytic contaminants in a commercial phenol oxidase preparation

The use of enzymes may improve the functional properties of various food ingredients. The aim of this study was to examine the effects of proteolytic contaminants in phenol oxidases on β-lactoglobulin (BLG). In the presence of Trametes versicolor laccase and Agaricus bisporus tyrosinase, both variants of BLG (A and B) underwent removal of a peptide from the N-terminus. The truncated forms were more susceptible to digestion by pepsin. The truncation of BLG resulted from contaminating proteases and not due to the action of phenol oxidases. The removal of N-terminal peptides proceeded quickly, while the rest of the globular protein remained resistant to proteolysis for up to 3 h. In the case of the application of enzymes in food bioprocessing, it may be important to carefully monitor the effects of contaminating proteases in enzyme preparations used.     

Drying of shiitake mushrooms in a vacuum dryer and optimization of the process by response surface methodology (RSM)

Optimization of shiitake mushroom vacuum drying process was the objective of this research. In order to produce dried sample with satisfactory shelf life, response surface methodology was used. Vacuum drying used to accomplish the task was previously described by the authors. The samples were dried at 46–74 °C and 20–580 mbar. As quality indicators of dried product, total solids, water activity, total phenolics, IC₅₀ and total color change were used. Response surface methodology resulted in optimal conditions of 57.1 °C and 100 mbar. In order to verify predictions and adequacy of the second-order polynomial models, separate validation experiments were conducted at optimum conditions. The predicted amount of total solids was 85.40 %, while a_w value was 0.615.     

Situation and perspectives of waste biomass application as energy source in Serbia

The Autonomous Province of Vojvodina is an autonomous province in the Republic of Serbia. It is located in the northern part of the country, in the Pannonia plain. Vojvodina is an energy-deficient province. The average yearly quantity of the cellulose wastes in Vojvodina amounts to about 9 millions tons barely in the agriculture, and the same potential on the level of Serbia is estimated to almost 13 million tons. Only minor part of straw is utilized, and almost two-thirds are incinerated on fields owing to the problems during plowing under. The large sector in Serbia utilizes only about 15% of straw, while the individual one utilizes about 50% of straw and 20% of cornstalks. Environment pollutions, abandonment of the utilization of at least of one-third of the yield and extermination of the natural resources, primarily of humus, represent very adverse results of such procedures. Main problems with respect to the profitable usage of straw and other post-harvest residues are high expenses of their collection (collecting, balling or some other manner of compression), transportation from production- to the usage cites, as well as their handling and storaging. The agricultural production in Serbia should be based on the system of farms. For the efficient farming, it is obvious to organize life of producer and of his family immediately close to the production capacities. For the agriculture development, it is obvious to create a system of premiums, efficient crediting and the elaborated tax system that could create a basis for the certitude of work, confidence and constant growth of production, together with the mentioned and other measures. As the result of the activities oriented to substitution of the classical energents with energy obtained from biomass, farm that is in a higher degree energetically independent should be created. In such case, farms should apply the basic principles of the cleaner manufacturing, as an integral part of the concept of the sustainable development.     

Biodiesel production by ultrasound-assisted transesterification: State of the art and the perspectives

In the present paper state-of-the art and perspectives of ultrasound-assisted (UA) biodiesel production from different oil-bearing materials using acid, base and enzyme catalysts are critically discussed. The ultrasound action in biodiesel production is primarily based on the emulsification of the immiscible liquid reactants by microturbulence generated by radial motion of cavitation bubbles and the physical changes on the surface texture of the solid catalysts generating new active surface area. The importance of ultrasound characteristics and other process variables for the biodiesel yield and the reaction rate is focused on. UA transesterification is compared with other techniques for biodiesel production. Several different developing methods reducing the biodiesel production costs such as the optimization of process factors, the development of the process kinetic models, the use of phase transfer catalysts, the application of the continuous process, the design of novel types of ultrasonic reactors and the in situ ultrasound application in transesterification of oily feedstocks are also discussed.     

Toxin-producing Ostreopsis cf. ovata are likely to bloom undetected along coastal areas

Mass appearances of the toxic dinoflagellate genus Ostreopsis are known to cause dangerous respiratory symptoms in humans exposed to aerosols. The outbreaks can appear in shallow marine waters of temperate regions around the globe. We followed a massive bloom event on a public beach on the northern Adriatic coast near Rovinj, Croatia. We identified the responsible species and the produced toxins as well as the dynamics of the event with respect to environmental conditions. Ostreopsis cf. ovata appeared in masses from September through October 2010 on a public beach near Rovinj, Croatia but stayed undetected by public health organizations. Respiratory symptoms were observed whenever humans were exposed to substrate samples containing large numbers of Ostreopsis cells. During the mass abundance of O. cf. ovata also exposure to the aerosols on the beach evoked respiratory symptoms in humans. Our measurements showed high cell abundances and high toxin contents with a stable relative contribution of putative Palytoxin and Ovatoxins a-e. Artificial beach structures proved to dramatically reduce settling of the observed Ostreopsis biofilm. Blooms like those reported herein have a high potential to happen undetected with a high potential of affecting the health of coastal human populations. Increased monitoring efforts are therefore required to understand the ecology and toxicology of those bloom events and reduce their negative impact on coastal populations.     

Biomass energy in Vojvodina: Market conditions,environment and food security

In this study, the policy, market conditions and food security of biomass energy sources are assessed for supplying the future needs of Vojvodina. The Autonomous Province of Vojvodina is an autonomous province in Serbia, containing about 27% of its total population according to the 2002 Census. It is located in the northern part of the country, in the Pannonia plain, in southeastern Europe.Vojvodina is an energy-deficient province. Vojvodina also has a large potential for renewable energy, especially energy from biomass (biodiesel and bio-ethanol). The lack of knowledge about renewable energy technologies by most policy-makers, potential consumers, and energy firm managers has played against renewable energy developments. The environmental impacts of programs that encourage biofuel production, farmland land requirements and the impacts on food production are also discussed, considering the life cycle analysis (LCA) as a tool.It is concluded that the rise in the use of biofuels is inevitable and that international cooperation, regulations and certification mechanisms must be established regarding the use of land, the mitigation of environmental and social impacts caused by biofuel production. It is also mandatory to establish appropriate working conditions and decent remuneration for workers of the biofuels production chain.     

Cleaner bioprocesses for promoting zero-emission biofuels production in Vojvodina

In this study, the policy, market conditions and food security of biomass energy sources are assessed for supplying the future needs of Vojvodina. The Autonomous Province of Vojvodina is an autonomous province in Serbia, containing about 27% of its total population according to the 2002 Census. It is located in the northern part of the country, in the Pannonia plain, in southeastern Europe. Vojvodina is an energy-deficient province. The incentives to invest human and financial resources in the research and development of cleaner bioprocesses are high, considering the benefits which might be achieved in terms of environment protection and manufacturing costs. In the near and medium tenu, the development of bioprocesses for waste recycling and resource recovery might be one of the most viable options, considering much research work has already been done. In Vojvodina, there are technological solutions that biofuels produced in a closed cycle, so that the quantity of waste reduced to a minimum. These solutions include the stillage (remainder after distillation) used for fattening cattle, and cattle excrement to produce biogas and manure as fertilizer. The energy required for the production of bioethanol is obtained combustion lignocelullose residual waste from the production of basic raw materials starch, or biogas. Ash from the burned biomass returned to soil as a source of minerals for plants and replacement of mineral fertilizer. Such a closed cycle is economical for small farms in Vojvodina.