Mitochondria: A mirror into cellular dysfunction in heart disease

Cardiovascular (CV) disease is the single most significant cause of morbidity and mortality worldwide. The emerging global impact of CV disease means that the goals of early diagnosis and a wider range of treatment options are now increasingly pertinent. As such, there is a greater need to understand the molecular mechanisms involved and potential targets for intervention. Mitochondrial function is important for physiological maintenance of the cell, and when this function is altered, the cell can begin to suffer. Given the broad range and significant impacts of the cellular processes regulated by the mitochondria, it becomes important to understand the roles of the proteins associated with this organelle. Proteomic investigations of the mitochondria are hampered by the intrinsic properties of the organelle, including hydrophobic mitochondrial membranes; high proportion of basic proteins (pI greater than 8.0); and the relative dynamic range issues of the mitochondria. For these reasons, many proteomic studies investigate the mitochondria as a discrete subproteome. Once this has been achieved, the alterations that result in functional changes with CV disease can be observed. Those alterations that lead to changes in mitochondrial function, signaling and morphology, which have significant implications for the cardiomyocyte in the development of CV disease, are discussed.     

An exploratory study of the accessibility of state government Web sites

This paper focuses on Web accessibility. Relevant legislation and judicial decisions are reviewed, and prior empirical research is presented. This study presents the results from the Web accessibility analysis of the home pages of the 50 states and the District of Columbia. It found that twenty-nine percent of the home pages do not meet the requirements for Conformance Level A Web accessibility. Only one state meets Conformance Level AA standards, which are the minimum standards recommended for Web accessibility, and no state meets Conformance Level AAA standards. This paper also discusses how to address common accessibility problems.

Direct synthesis of hydrogen peroxide from H2 and O2 using zeolite-supported Au-Pd catalysts

The direct synthesis of hydrogen peroxide from H₂ and O₂ using zeolite-supported Au-Pd catalysts is described using two zeolites, ZSM-5 and zeolite Y, using an impregnation method of preparation. The addition of Pd to Au for these catalysts significantly enhances the productivity for hydrogen peroxide. The use of zeolites as a support for Au-Pd gives higher rates of hydrogen peroxide formation when compared with alumina-supported Au catalysts prepared using a similar method. The addition of metals other than Pd is also investigated, but generally Au-Pd catalysts give the highest activity for the synthesis of hydrogen peroxide. The addition of Ru and Rh have no significant effect, but the addition of Pt does enhance the activity for the selective formation of hydrogen peroxide.     

Evidence for internal phosphorus loading,hypoxia and effects on phytoplankton in partially polymictic Lake Simcoe,Ontario

Hypoxia and cyanobacterial blooms were extensive in Lake Simcoe during the 1980s and are still a problem to a lesser degree despite extensive nutrient load reduction from the catchment basin. The continuing signs of productivity indicate a potential internal phosphorus (P) source. Internal P load, as a redox-dependent release from bottom sediments, is hard to determine in a large, relatively shallow and partially unstratified lake such as Lake Simcoe. Of the lake's three major basins, only Kempenfelt Bay stratifies long enough to develop hypoxia in the stagnant summer hypolimnion. The following indications of sediment P release are available from historic data: 1) hypolimnetic hypoxia still occurs in Kempenfelt Bay although the hypoxic factor (number of days that an area equal to the bay's surface area is overlain by water of ≤ 2 mg/L dissolved oxygen, DO) has decreased substantially and significantly from 15.8 d/yr (1980–1994) to 4.0 d/yr (1995–2011); 2) hypoxic factors for other lake sections and at different DO levels also indicate widespread hypoxia; 3) concentrations of redox dependent metals, Fe and Mn, increase with depth; and 4) euphotic zone P and chlorophyll concentrations increase and water clarity decreases during fall turnover. Cyanobacterial blooms appear to occur in response to internal load as supported by occasional cyanobacteria counts. These indicators provide evidence that internal loading is likely occurring and affecting the water quality in Lake Simcoe. We expect that further monitoring, specific for internal load, will corroborate these results.     

Assessment of fish mercury levels in the upper St. Lawrence River,Canada

The stretch of the St. Lawrence River near Cornwall, Ontario, Canada, between the Moses–Saunders power dam to the eastern outlet of Lake St. Francis in Québec, is currently listed as an Area of Concern (AOC), and has restrictive fish consumption advisories in place, largely due to high mercury levels. This study examined long-term temporal trends of mercury concentrations in northern pike, smallmouth bass, walleye and yellow perch from the St. Lawrence River, including the Cornwall AOC. In addition, differences in fish mercury concentrations among river sections were compared for each species using historical (1975–1979) and recent (2000–2011) data. Statistically significant declines in mercury concentrations were observed in all river sections for yellow perch (~ 40%), as well as in northern pike and walleye in at least one river section. For the river section encompassing the AOC, recent mercury concentrations are 33–59% lower than historical mercury concentrations for all four species. Further, a comparison of recent mercury concentrations between the AOC river section and other Ontario lakes/rivers suggests that AOC mercury values are within the 75th percentile of values for other Ontario water bodies in three of the four species considered. However, current fish mercury values for the AOC river section remain higher than one or more upstream river sections. These results indicate that recovery is still ongoing in this AOC.     

Critical thinking in E-learning environments

One of the primary aims of higher education in today’s information technology enabled classroom is to make students more active in the learning process. The intended outcome of this increased IT-facilitated student engagement is to foster important skills such as critical thinking used in both academia and workplace environments. Critical thinking (CT) skills entails the ability(ies) of mental processes of discernment, analysis and evaluation to achieve a logical understanding. Critical thinking in the classroom as well as in the workplace is a central theme; however, with the dramatic increase of IT usage the mechanisms by which critical thinking is fostered and used has changed. This article presents the work and results of critical thinking in a virtual learning environment. We therefore present a web-based course and we assess in which parts of the course, and to what extent, critical thinking was perceived to occur. The course contained two categories of learning modules namely resources and interactive components. Critical thinking was measured subjectively using the ART scale. Results indicate the significance of “interactivity” in what students perceived to be critical-thinking-oriented versus online material as a resource. Results and opportunities that virtual environments present to foster critical thinking are discussed.     

The changing face of bullying: An empirical comparison between traditional and internet bullying and victimization

Electronic aggression, or cyberbullying, is a relatively new phenomenon. As such, consistency in how the construct is defined and operationalized has not yet been achieved, inhibiting a thorough understanding of the construct and how it relates to developmental outcomes. In a series of two studies, exploratory and confirmatory factor analyses (EFAs and CFAs respectively) were used to examine whether electronic aggression can be measured using items similar to that used for measuring traditional bullying, and whether adolescents respond to questions about electronic aggression in the same way they do for traditional bullying. For Study I (n = 17 551; 49% female), adolescents in grades 8-12 were asked to what extent they had experience with physical, verbal, social, and cyberbullying as a bully and victim. EFA and CFA results revealed that adolescents distinguished between the roles they play (bully, victim) in a bullying situation but not forms of bullying (physical, verbal, social, cyber). To examine this further, Study II (n = 733; 62% female), asked adolescents between the ages of 11 and 18 to respond to questions about their experience sending (bully), receiving (victim), and/or seeing (witness) specific online aggressive acts. EFA and CFA results revealed that adolescents did not differentiate between bullies, victims, and witnesses; rather, they made distinctions among the methods used for the aggressive act (i.e. sending mean messages or posting embarrassing pictures). In general, it appears that adolescents differentiated themselves as individuals who participated in specific mode of online aggression, rather than as individuals who played a particular role in online aggression. This distinction is discussed in terms of policy and educational implications.     

Are Cyberbullies really bullies? An investigation of reactive and proactive online aggression

Cyberbullying, or online aggression, is an issue of increasing concern, however little research has been conducted on the motivations underlying this form of aggression. Using a mixed-method approach, by means of survey and interview data, we explored whether online aggressive acts were motivated by proactive (intentionally aggressing to obtain a resource or a goal), and/or reactive (aggression that occurs in reaction to provocation) reasons. Participants for the survey portion of the study included 733 adolescents between the ages of 10 and 18, while a subset of 15 adolescents participated in semi-structured interviews. Factor analysis revealed that, in contrast to traditional forms of bullying, adolescents do not identify themselves according to the role they played in an internet aggressive situation (i.e. bully, victim, witness), but according to the method of aggression they used (i.e. sending mean messages, posting embarrassing photos, and developing hostile websites). More interestingly, regression analyses demonstrated that motivations for aggressing online also varied according to method of aggression rather than role. For example, adolescents who chose to aggress by posting mean messages or posting embarrassing photos were more likely to do so for reactive reasons, while adolescents who spent time creating hostile websites did so for proactive reasons.     

Effects of Sexually Objectifying Media on Self-Objectification and Body Surveillance in Undergraduates: Results of a 2-Year Panel Study

This study used objectification theory (B. L. Fredrickson & T.-A. Roberts, 1997) to predict that the media's insidious practice of objectifying bodies socializes individuals to take an outsider's perspective on the physical self (i.e., self-objectify) and to habitually monitor their appearance (i.e., engage in body surveillance). To test these hypotheses, a 2-year panel study using an undergraduate sample was conducted. Cross-lagged path models showed that exposure to sexually objectifying television measured during Year 1 increased trait self-objectification (trait SO) during Year 2 for both women and men. At the same time, trait SO during Year 1 decreased exposure to sexually objectifying television during Year 2, suggesting that both male and female participants selectively avoided sexually objectifying television based on antecedent trait SO. Moreover, exposure to sexually objectifying television and magazines increased body surveillance for men only. The discussion focuses on the process by which the media create body-focused perceptions.     

Adsorption of RDX and HMX in Rapid Small-Scale Column Tests: Implications for Full-Scale Adsorbers

The high explosive (HE) compounds royal demolition explosive or hexahydro-1,3,5-trinitro-1,3,5-triazocine (RDX) and high melting explosive or octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine (HMX) have been detected as groundwater contaminants at many military facilities. This research evaluated adsorption of RDX and HMX with granular activated carbon (GAC) to provide guidance for the design and operation of GAC adsorbers for treatment of HE-contaminated groundwater. Five GACs were screened using rapid small-scale column tests (RSSCTs), after which additional tests were performed with the two GACs that most effectively treated mixtures of RDX and HMX (Calgon F400 and Northwestern LB-830). GAC service life as a function of empty-bed contact time (EBCT) was determined using RSSCTs for a range of simulated full scale EBCTs with influent concentrations of 2,200 μg RDX/L and 350 μg HMX/L. Increasing the influent concentration of either contaminant significantly reduced the predicted service life, as did preloading GAC with groundwater natural organic matter. In batch isotherm tests, RDX was less adsorbable than HMX under all conditions studied. Concurrent loading of natural organic matter reduced the Freundlich K for RDX, whereas adsorption of HMX was not affected. Of the GACs tested, Calgon F400 most effectively removed RDX and HMX.