A superhydrophilic titanium implant functionalized by ozone gas modulates bone marrow cell and macrophage responses

Bone-forming cells and M? play key roles in bone tissue repair. In this study, we prepared a superhydrophilic titanium implant functionalized by ozone gas to modulate osteoconductivity and inhibit inflammatory response towards titanium implants. After 24 h of ozone gas treatment, the water contact angle of the titanium surface became zero. XPS analysis revealed that hydroxyl groups were greatly increased, but carbon contaminants were largely decreased 24 h after ozone gas functionalization. Also, ozone gas functionalization did not alter titanium surface topography. Superhydrophilic titanium (O₃–Ti) largely increased the aspect ratio, size and perimeter of cells when compared with untreated titanium (unTi). In addition, O₃–Ti facilitated rat bone marrow derived MSCs differentiation and mineralization evidenced by greater ALP activity and bone-like nodule formation. Interestingly, O₃–Ti did not affect RAW264.7 M? proliferation. However, naive RAW264.7 M? cultured on unTi produced a two-fold larger amount of TNFα than that on O₃–Ti. Furthermore, O₃–Ti greatly mitigated proinflammatory cytokine production, including TNFα and IL-6 from LSP-stimulated RAW264.7 M?. These results demonstrated that a superhydrophilic titanium prepared by simple ozone gas functionalization successfully increased MSCs proliferation and differentiation, and mitigated proinflammatory cytokine production from both naive and LPS-stimulated M?. This superhydrophilic surface would be useful as an endosseous implantable biomaterials and as a biomaterial for implantation into other tissues.     

Coupled temperature and moisture effects on the tensile behavior of strain hardening cementitious composites (SHCC) reinforced with PVA fibers

This paper reports the experimental findings on the tensile behavior of strain-hardening cement-based composites (SHCC). The composites were subjected to the combined effects of elevated temperatures and internal moisture condition. Uniaxial tensile tests on dumbbell-shaped SHCC specimens with in situ temperature control were performed at 22, 60 and 100 °C. In addition, the effect of the internal humidity of SHCC (95, 50, 20 and 0%) coupled to the elevated temperatures was investigated. It was shown that the tensile strength decreases and the strain capacity increases with an increase in temperature. The influence of the internal moisture conditions was more significant in high temperatures. The strain capacity reduced significantly with a decrease in the humidity level. The crack pattern of the SHCC specimens was determined. Furthermore, single fiber pullout tests were performed under the considered high temperatures condition. Finally, the results are discussed based on the thermogravimetry analysis of the PVA fiber, alterations on its microstructure and surface coating.     

Recommendation of RILEM TC 238-SCM: determination of the degree of reaction of siliceous fly ash and slag in hydrated cement paste by the selective dissolution method

The performance of slag and fly ash in hydrated cementitious materials depends on the degree of reaction developed at the evaluated age. Several methods for the determination of the reaction degree of supplementary cementitious materials are available, among which the selective dissolution method is one of methods developed the earliest. This is a direct method that aims to quantify the amount of unreacted slag or fly ash in the sample by applying a selective acid attack. The degree of reaction is obtained from the comparison between the remaining unreacted SCM, which should not dissolve, and the total amount initially included in the mix. This recommendation indicates suitable procedures for computing the degree of reaction by selective dissolution of cement pastes containing slag and fly ash. Specific considerations are indicated for necessary corrections due to the imperfect selective dissolution when the procedure is applied to hydrated cement paste.     

Secondary bioreceptivity of granite: effect of salt weathering on subaerial biofilm growth

Salt crystallisation is a very common and powerful weathering agent that can modify the petrophysical properties of building stone such as granite. In addition, the weathering can affect the susceptibility of the stone to biological colonisation. The aims of the present study were to examine the properties of a granite weathered by sodium chloride crystallisation and to evaluate the effects of the weathering on the secondary bioreceptivity of the stone to subaerial phototrophic biofilms. For this purpose, granite samples were subjected to a laboratory-based accelerated salt weathering test, and changes in weight, open porosity, bulk density, capillary water content, abrasion pH and surface roughness of the samples were determined. Samples of both weathered and non-weathered granite were then inoculated with a multi-species phototrophic culture derived from a natural subaerial biofilm and incubated under standardised laboratory conditions for 3 months. The weight loss produced by the weathering process was consistent with significant changes in abrasion pH and surface roughness. The bioreceptivity of the stone was also altered. According to the bioreceptivity index, the granite under study was characterised by ‘mild primary bioreceptivity’, but ‘high secondary bioreceptivity’ after the salt weathering process. Study of the secondary bioreceptivity of stone materials can provide very useful information about response to weathering effects, and the findings can be used to improve the selection of materials for building purposes.     

Nonlinearity of bituminous mixtures

This paper presents an experimental characterization of the strain dependency of the complex modulus of bituminous mixtures for strain amplitude levels lower than about \(110~\upmu\mbox{m}/\mbox{m}\). A series of strain amplitude sweep tests are performed at different temperatures (8, 10, 12 and 14°C) and frequencies (0.3, 1, 3 and 10 Hz), during which complex modulus is monitored. For each combination of temperature and frequency, four maximum strain amplitudes are targeted (50, 75, 100 and \(110~\upmu\mbox{m}/\mbox{m}\)). For each of them, two series of 50 loading cycles are applied, respectively at decreasing and increasing strain amplitudes. Before each decreasing strain sweep and after each increasing strain sweep, 5 cycles are performed at constant maximum targeted strain amplitude.Experimental results show that the behavior of the studied material is strain dependent. The norm of the complex modulus decreases and phase angle increases with strain amplitude. Results are presented in Black and Cole–Cole plots, where characteristic directions of nonlinearity can be identified. Both the effects of nonlinearity in terms of the complex modulus variation and of the direction of nonlinearity in Black space seem to validate the time–temperature superposition principle with the same shift factors as for linear viscoelasticity.The comparison between results obtained during increasing and decreasing strain sweeps suggests the existence of another phenomenon occurring during cyclic loading, which appears to systematically induce a decrease of the norm of the complex modulus and an increase of the phase angle, regardless of the type of the strain sweep (increasing or decreasing).     

Bending impact on the performance of a flexible Li4Ti5O12-based all-solid-state thin-film battery

The growing demand of flexible electronic devices is increasing the requirements of their power sources. The effect of bending in thin-film batteries is still not well understood. Here, we successfully developed a high active area flexible all-solid-state battery as a model system that consists of thin-film layers of Li₄Ti₅O₁₂, LiPON, and Lithium deposited on a novel flexible ceramic substrate. A systematic study on the bending state and performance of the battery is presented. The battery withstands bending radii of at least 14 mm achieving 70% of the theoretical capacity. Here, we reveal that convex bending has a positive effect on battery capacity showing an average increase of 5.5%, whereas concave bending decreases the capacity by 4% in contrast with recent studies. We show that the change in capacity upon bending may well be associated to the Li-ion diffusion kinetic change through the electrode when different external forces are applied. Finally, an encapsulation scheme is presented allowing sufficient bending of the device and operation for at least 500 cycles in air. The results are meant to improve the understanding of the phenomena present in thin-film batteries while undergoing bending rather than showing improvements in battery performance and lifetime.     

GraftFast Surface Engineering to Improve MOF Nanoparticles Furtiveness

Controlling the outer surface of nanometric metal–organic frameworks (nanoMOFs) and further understanding the in vivo effect of the coated material are crucial for the convenient biomedical applications of MOFs. However, in most studies, the surface modification protocol is often associated with significant toxicity and/or lack of selectivity. As an alternative, how the highly selective and general grafting GraftFast method leads, through a green and simple process, to the successful attachment of multifunctional biopolymers (polyethylene glycol (PEG) and hyaluronic acid) on the external surface of nanoMOFs is reported. In particular, effectively PEGylated iron trimesate MIL‐100(Fe) nanoparticles (NPs) exhibit suitable grafting stability and superior chemical and colloidal stability in different biofluids, while conserving full porosity and allowing the adsorption of bioactive molecules (cosmetic and antitumor agents). Furthermore, the nature of the MOF–PEG interaction is deeply investigated using high‐resolution soft X‐ray spectroscopy. Finally, a cell penetration study using the radio‐labeled antitumor agent gemcitabine monophosphate (³H‐GMP)‐loaded MIL‐100(Fe)@PEG NPs shows reduced macrophage phagocytosis, confirming a significant in vitro PEG furtiveness.     

Solid biofuels in Mexico: a sustainable alternative to satisfy the increasing demand for heat and power

Bioenergy is the largest renewable energy source in Mexico with an estimated 4–9% of total current energy demand. There are large uncertainties and contrasting estimates regarding its current extent and end-uses, particularly with traditional uses. However, a large potential exists to improve the efficiency of existing uses and, at the same time, to diversify the use of SBF in the industrial and power sectors. This paper aims at: providing the first updated and comprehensive estimate of current SBF demand in Mexico including traditional and modern uses; providing a consistent estimate of actual SBF supply potential; estimating the total potential substitution of fossil fuels that could be achieved by SBF considering an integrated “modernization scenario”; and finally describing the main barriers limiting SBF to fully triggering its potential. Results show that current SBF consumption reached 481 PJ/yr in 2015; SBF supply potential reaches 3622 PJ/yr, out of which 883 PJ/yr could be used to substitute up to 29% of current demand of FF, mitigating 66 MtCO_2e/yr of greenhouse gas (GHG) emissions, or near 88 MtCO_2e/yr if mitigation from traditional uses is added.     

Technologies and logistics for phosphorus recovery from livestock waste

Phosphorus (P) runoff from livestock waste can trigger algal blooms that adversely affect aquatic life and human health. One strategy to mitigate this problem is to install nutrient recovery technologies that concentrate and mobilize nutrients from nutrient-rich regions to nutrient-deficient ones. We present supply chain design formulations to identify optimal types and locations for P recovery technologies. The formulations capture trade-offs in transportation costs, technology efficiency, investment/operational costs, revenue collected from different recovered products (struvite and nutrient cakes), and environmental impact. Our computational framework is used to analyze the impact of different scenarios for market prices of recovered products, recovery yields, and remediation costs. We find that transportation of waste alone (without any processing) can achieve significant reductions in environmental impact at low cost, but cannot achieve economic sustainability in the long run due to the lack of direct revenue streams. Mechanical separation technologies that recover P in the form of nutrient cakes are low-cost solutions that can achieve high environmental benefits and reduced transportation costs, but revenues are also limited due to low values of the cakes. Struvite crystallization in fluidized beds is found to be a highly attractive option under current struvite prices, but economic sustainability is strongly dependent on yield recoveries (which are currently highly uncertain).