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41.
42.
Asako Yamamoto Lingli Yang Yasutaka Kuroda Jiao Guo Lanting Teng Daisuke Tsuruta Ichiro Katayama 《International journal of molecular sciences》2021,22(1)
As the outermost barrier of the body, skin is a major target of oxidative stress. In the brain, estrogen has been reported synthesized locally and protects neurons from oxidative stress. Here, we explored whether estrogen is also locally synthesized in the skin to protect from oxidative stress and whether aberrant local estrogen synthesis is involved in skin disorders. Enzymes and estrogen receptor expression in skin cells were examined first by quantitative real-time PCR and Western blot analyses. Interestingly, the estrogen synthesis enzyme was mainly localized in epidermal keratinocytes and estrogen receptors were mainly expressed in melanocytes among 13 kinds of cultured human skin cells. The most abundant estrogen synthesis enzyme expressed in the epidermis was 17β-hydroxysteroid dehydrogenase 1 (HSD17β1) localized in keratinocytes, and the most dominant estrogen receptor expressed in the epidermis was G protein-coupled estrogen receptor 1 (GPER1) in melanocytes. To investigate whether keratinocyte-derived estradiol could protect melanocytes from oxidative stress, cultured human primary epidermal melanocytes (HEMn-MPs) were treated with H2O2 in the presence or absence of 17β estradiol or co-cultured with HSD17β1 siRNA-transfected keratinocytes. Keratinocyte-derived estradiol exhibited protective effects against H2O2-induced cell death. Further, reduced expression of HSD17β1 in the epidermis of skin from vitiligo patients was observed compared to the skin from healthy donors or in the normal portions of the skin in vitiligo patients. Our results suggest a possible new target for interventions that may be used in combination with current therapies for patients with vitiligo. 相似文献
43.
由简到繁地推导出笼型异步电机在各种配电形式下的全压起动判断公式,从中得出满足全压起动条件的影响因素和建议措施.结合实际工程案例,为设计人员判断复杂配电回路中的电机是否能全压起动提供了思路和参考方法. 相似文献
44.
通用电子手册数据库录入软件的关键技术 总被引:1,自引:0,他引:1
文章主要讨论了关于通用电子手册数据库录入软件的组成和功能,较为详细地讨论了实际开发中几个关键技术的解决方案.实际设计完成了通用电子手册数据库录入软件模块,并应用于实际工作中,运行良好. 相似文献
45.
对MTG区熔生长法,慢冷法,粉末熔融生长法这3种方法制备的熔融织构YBCO块样品进行了交流磁化率和磁阻的测量。根据实验结果计算了样品的钉扎势,并且发现3种方法制备的样品在150 mT外磁场下均显示出基本消除弱连接的特性;粉末融熔生长法所制样品中有较纯净123相和较好织构,质量最好。 相似文献
46.
在分析了网络化定制关键技术的基础上,针对性地对信息映射方法和产品族建进行了研究。提出了多层结构信息映射模型,以及综合类元件和参数化特征建模的双层复合产品族模型。结合本方法和以往网络化制造领域中所取得的成果,实现了较完整的基于Web的个性化定制过程。 相似文献
47.
Gaussian type models have been established according to the real detected voltage of magnetically insulated ion diode and intense pulsed ion beam density near the focus of the diode. The energy deposition of Al target irradiated by intense pulsed ion beam has been simulated by Monte Carlo method based on these models. The results of energy depositions of the irradiation of single energy ions and the Gaussian distribution IPIB have been obtained. Especially cases of ion beam irradiating Al target at different angles on one dimension and two dimensions are discussed. The evolution processes of the energy deposition of ions in target have been simulated during a pulse. In addition, incident angles of H+ ions and C+ ions ranging from 20 to 40° have been researched. We obtained the conclusions that C+ ions of the beam affect the physical properties of near surface region, but H+ ions affect the deeper layer. The energy of intense pulsed ion beam mainly deposits on target surface, so melting and evaporation begin both from target surface. 相似文献
48.
采用人胎盘血为原料,利用Sephadex G-200,DEAE-Sephadex A-50层析,Sepharose 4B反相免疫亲和层析技术,获得妊娠相关血浆蛋白A(PAPP-A)纯品,经8.0%聚丙烯酰胺凝胶电泳、免疫电泳、双向免疫扩散证明,所提取的PAPP-A纯品为单一成分。免疫家兔获得抗血清,其效价在1:16以上。 相似文献
49.
锦屏水电站锦屏山隧道开挖中面临高压大流量地下水、且富水地段长(约占隧道全长的40%)的难题。为安全、可靠地进行隧道施工,以注浆材料的适应性、超前帷幕注浆的参数及工艺为研究对象,对浆液做了大量配合比试验,分析其流动性、物理力学性能和凝结时间。研究结果表明:用纯水泥进行单液浆帷幕注浆,其可注性好,可大大地提高注浆加固效果;纯水泥+双液浆对局部小的渗漏水具有很好的封堵效果。对于低于2 MPa的压力水,注浆压力取2~3倍的静水压力;对于高于5 MPa的压力水,注浆压力取净水压力加2~3 MPa。对于在2~5 MPa之间的压力水,注浆压力可灵活选取,但不宜超过7 MPa,注浆应采用孔内循环灌注的方式。地下水发生在断层破碎带时,超前帷幕注浆宜采用全断面帷幕;若出水段以溶蚀裂隙、断层、管道为主宜采用有针对性的局部超前帷幕注浆。该技术成功解决了锦屏山隧道高压大流量地下水封堵的难题,对富水地层地下隧洞施工具有重要参考价值。 相似文献
50.
Carbon/carbon composites were made through the pyrolysis of stabilized PAN felt and phenolic resin with the addition of 5 or 10 wt % carbon black to the matrix and then heat treatment at 600–2500°C. The effects of adding carbon black to the matrix precursor on the physical properties, microstructure, and mechanical properties of the resultant composites were investigated. Adding carbon black not only reduced the weight loss but also limited the shrinkage of the resultant composites. Adding carbon black also accelerated the formation of carbon basal planes in the matrix. At 2500°C, the crystalline stacking height in the composite with 10 wt % added carbon black was 200% greater than that with no additive. The flexural strength of the composite also increased from 15 to 42 MPa (almost 300%). © 2006 Wiley Periodicals, Inc. J Appl Polym Sci 102: 333–337, 2006 相似文献