Spray drying conditions for orange juice incorporated with lactic acid bacteria

This work aimed to develop an orange juice powder by spray drying with lactic acid bacteria (Lactobacillus plantarum 299v and Pediococcus acidilactici HA‐6111‐2), testing their survival both during drying and storage (room temperature and 4 °C). Initially, the best conditions for spray drying were chosen to allow the best survival of each LAB: (i) inlet air temperature of 120 °C and (ii) 0.5:2 ratio of the orange juice soluble solids and drying agent added (prebiotics: 10 DE maltodextrin or gum Arabic). Survival of LAB was not affected by drying process, and it was higher when cultures were stored at 4 °C. A slightly higher protection was conferred by 10 DE maltodextrin, in the case of L. plantarum and at 4 °C. Pediococcus acidilactici was more resistant during storage at 4 °C, with logarithmic reductions lower than 1 log‐unit. It was demonstrated that it is possible to produce a functional nondairy product, orange juice powder supplemented with prebiotic compounds, containing viable LAB for at least 7 months, when stored at 4 °C.     

Use of Ozonated Water for Disinfecting Gastrointestinal Endoscopes

The reprocessing of endoscopes is a complex procedure due to their structural design. In the constant search for new antimicrobial substances, recent studies with ozone have yielded great benefits. The present study evaluated the effects of ozonated water used to disinfect endoscopes comparing its efficacy with the conventional technique (2% glutaraldehyde). According to the results obtained, when ozonated water was used (330 mg.min.L^?1), induced a 2 log reduction of the viable microorganisms under the conditions tested. Ozonated water was a potent gastrointestinal endoscopic sanitizer, suggesting it is a feasible alternative for disinfection.     

RF Power Amplifier Behavioral Modeling Based on Takenaka–Malmquist–Volterra Series

In this paper, a Takenaka–Malmquist–Volterra (TMV) model structure is employed to improve the approximations in the low-pass equivalent behavioral modeling of radio frequency (RF) power amplifiers (PAs). The Takenaka–Malmquist basis generalizes the orthonormal basis functions previously used in this context. In addition, it allows each nonlinearity order in the expanded Volterra model to be parameterized by multiple complex poles (dynamics). The state-space realizations for the TMV models are introduced. The pole sets for the TMV model and also for the previous Laguerre–Volterra (LV) and Kautz–Volterra (KV) models are obtained using a constrained nonlinear optimization approach. Based on experimental data measured on a GaN HEMT class AB RF PA excited by a WCDMA signal, it is observed that the TMV model reduces the normalized mean-square error and the adjacent channel error power ratio for the upper adjacent channel (upper ACEPR) by 1.6 dB when it is compared to the previous LV and KV models under the same computational complexity.     

Nanosized dispersions based on chitosan and NaPSS

Interpolyelectrolyte complexes (IPECs) were obtained by the solution mixing method from chitosan and poly(sodium 4-styrenesulfonate), NaPSS. XRD, FTIR spectroscopy, and thermogravimetric analysis indicated that IPEC formation inhibits the occurrence of crystalline regions in the resultant solid IPECs. Turbidimetry, viscometry, conductometry, and zeta potential measurements showed that at a sulfonate to aminium molar ratio = 1, the process of IPEC production is optimum. Average hydrodynamic diameters, calculated from DLS measurements, showed that IPEC formation occurs in two stages: first there is a decrease in macromolecular dimensions, as sulfonate to aminium molar ratio is increased. At a characteristic sulfonate to aminium molar ratio, soluble IPEC structures collapse to form phase segregated clusters that begin to nucleate the formation of larger, insoluble, IPEC particles.     

Effects of supplemental progesterone after artificial insemination on expression of interferon-stimulated genes and fertility in dairy cows

The objectives of the current study were to evaluate the effects of supplemental progesterone after artificial insemination (AI) on expression of IFN-stimulated genes (ISG) in blood leukocytes and fertility in lactating dairy cows. Weekly cohorts of Holstein cows were blocked by parity (575 primiparous and 923 multiparous) and method of insemination (timed AI or AI on estrus) and allocated randomly within each block to untreated controls, a controlled internal drug release (CIDR) containing 1.38 g of progesterone from d 4 to 18 after AI (CIDR4), or a CIDR on d 4 and another on d 7 after AI and both removed on d 18 (CIDR4+7). Blood was sampled to quantify progesterone concentrations in plasma and mRNA expression in leukocytes for the ubiquitin-like IFN-stimulated gene 15-kDa protein (ISG15) and receptor transporter protein-4 (RTP4) genes. Pregnancy was diagnosed on d 34 ± 3 and 62 ± 3 after AI. Treatment increased progesterone concentrations between d 5 and 18 after AI in a dose-dependent manner (control = 3.42, CIDR4 = 4.97, and CIDR4+7 = 5.46 ng/mL). Cows supplemented with progesterone tended to have increased luteolysis by d 19 after AI (control = 17.2; CIDR4 = 29.1; CIDR4+7 = 30.2%), which resulted in a shorter AI interval for those reinseminated after study d 18. Pregnancy upregulated expression of ISG in leukocytes on d 19 of gestation, but supplementing progesterone did not increase mRNA abundance for ISG15 and RTP4 on d 16 after insemination and tended to reduce mRNA expression on d 19 after AI. For RTP4 on d 19, the negative effect of supplemental progesterone was observed only in the nonpregnant cows. No overall effect of treatment was observed on pregnancy per AI on d 62 after insemination and averaged 28.6, 32.7, and 29.5% for control, CIDR4, and CIDR4+7, respectively. Interestingly, an interaction between level of supplemental progesterone and method of AI was observed for pregnancy per AI. For cows receiving exogenous progesterone, the lower supplementation with CIDR4 increased pregnancy per AI on d 62 in cows inseminated following timed AI (CIDR4 = 39.2; CIDR4+7 = 27.5%); in those inseminated following detection of estrus, however, the use of a second insert on d 7 resulted in greater pregnancy per AI (CIDR4 = 26.9; CIDR4+7 = 31.5%). Pregnancy loss did not differ among treatments. Supplemental progesterone post-AI using a single intravaginal insert on d 4 was beneficial to pregnancy in cows inseminated following timed AI, but incremental progesterone with a second insert on d 7 did not improve fertility of dairy cows.     

Short communication: Passive shedding of Mycobacterium avium ssp. paratuberculosis in commercial dairy goats in Brazil

Goat farming is a low-cost alternative to dairy production in developing countries. In Brazil, goat production has increased in recent years due in part to the implementation of programs encouraging this activity. Mycobacterium avium ssp. paratuberculosis (MAP) is the causative agent of paratuberculosis, a disease that causes chronic granulomatous enteritis in ruminants, but MAP transmission dynamics are still poorly understood in goats. In a previously published study of our research group, 10 dairy goat farms (467 animals) from Minas Gerais state were analyzed for MAP detection; 2 fecal cultures and 11 milk samples tested positive for MAP by conventional PCR and were confirmed by sequencing. Because no clinical signs were observed over 1 yr of monitoring, we hypothesized that these MAP-positive goats could be passive shedders. Thus, in the present study, 4 positive goats (4/13) from the previous study were purchased and feces and milk samples were collected for evaluation (twice, with an interval of 3 mo between tests) by culture of MAP, IS900 PCR, or both. All analyses were negative for MAP. At the last time point, blood samples were collected for ELISA, the animals were killed, and tissues collected for tissue culture and histopathology. At necropsy, no macroscopic lesions related to paratuberculosis were observed. Similarly, no histological changes were observed and MAP in samples stained by Ziehl–Neelsen was not detected. These animals were characterized as potential passive shedders with upward contamination of the teat canal by MAP. This is the first report of the passive shedding phenomenon in goats in Brazil and it highlights the importance of identifying these animals for control programs and to ensure the quality of dairy products.     

Algorithm transformation methods to reduce the overhead of software-based fault tolerance techniques

This paper introduces a framework that tackles the costs in area and energy consumed by methodologies like spatial or temporal redundancy with a different approach: given an algorithm, we find a transformation in which part of the computation involved is transformed into memory accesses. The precomputed data stored in memory can be protected then by applying traditional and well established ECC algorithms to provide fault tolerant hardware designs. At the same time, the transformation increases the performance of the system by reducing its execution time, which is then used by customized software-based fault tolerant techniques to protect the system without any degradation when compared to its original form. Application of this technique to key algorithms in a MP3 player, combined with a fault injection campaign, show that this approach increases fault tolerance up to 92%, without any performance degradation.     

An Improved NER Methodology to the Portuguese Language

Mobile Networks and Applications - The text mining process typically involves the application of natural language processing (NLP) techniques, in order to obtain important information and extract...