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11.
Antonio Eduardo Nicácio Eliza Mariane Rotta Joana Schuelter Boeing Érica Oliveira Barizão Elza Kimura Jesui Vergilio Visentainer Liane Maldaner 《Food Analytical Methods》2017,10(8):2718-2728
Fruits have been the focus of several studies aimed at finding new antioxidant sources for protection against the damage caused by reactive species. In this study, the antioxidant activity and the presence of phenolic compounds in all parts (peel, pulp, and seeds) of Eugenia involucrata DC. fruits were evaluated. DPPH·, ABTS·+, and ORAC methods were used to determine the antioxidant activity, and an UHPLC-MS/MS method was developed for determining the phenolic compounds (gallic, chlorogenic, ferulic, p-coumaric and ellagic acids, quercetin, and myricetin). In the determination of both antioxidant activity and phenolic composition, the efficiency of solvents with different polarities—methanol/H2O (80:20, v/v), ethanol/H2O (80:20, v/v), methanol/acidified water with phosphoric acid pH 3.00 (80:20, v/v), and ethyl acetate—for the extraction of the phenolic compounds, was also evaluated. All parts of E. involucrata fruits showed antioxidant activity, in the range of 36.68 ± 1.44 to 873.87 ± 18.24 μmol TE g?1, being the highest values found in the seeds and peel when more polar extraction solvents were used. Six, five, and three phenolic compounds were identified and quantified in the pulp, peel, and seeds, respectively, with the highest abundance as p-coumaric acid (14 ± 2 mg kg?1) in the pulp, quercetin (47 ± 5 mg kg?1) in the peel, and gallic acid (74 ± 4 mg kg?1) in the seeds, also when more polar solvents were used. Although antioxidant activity methods suggested that the peel and seeds have more antioxidant potential, a wider variety of compounds were determined in the pulp. 相似文献
12.
Sandra Borges Joana Barbosa Joana Silva Ana M. Gomes Manuela Pintado Cristina L. M. Silva Alcina M. M. B. Morais Paula Teixeira 《International Journal of Food Science & Technology》2016,51(2):381-388
The aim of this study was to develop fruit powders (apple, banana and strawberry) enriched with a probiotic strain (Lactobacillus plantarum 299v). Two methodologies were proposed: (i) drying of the fruit with probiotic culture incorporated (by convection) or (ii) drying of fruit (by convection) and addition of spray‐dried probiotic culture. In the first methodology, processing caused a notable reduction in probiotic viable counts in apple, but this reduction was lower during drying of banana and strawberry. A large reduction in viable cells was also recorded during storage. In the second methodology, the survival of L. plantarum 299v was considerably higher during spray‐drying, and fruit powders with a microbial content suitable for a probiotic food (108–109 cfu g?1) were obtained. The fruit powders incorporating L. plantarum 299v can be stored at 4 °C or at room temperature, for at least 3 months. This preliminary study demonstrated that fruit powders are good carriers of probiotic cultures, but the techniques used to produce them should be carefully considered. 相似文献
13.
Joana T. Pimenta Carlos Gonçalves Loic Hiliou Jorge F. J. Coelho Fernão D. Magalhães 《Journal of Coatings Technology and Research》2016,13(2):227-238
This study investigates the role of the polymeric binder on the properties and performance of an intumescent coating. Waterborne resins of different types (vinylic, acrylic, and styrene-acrylic) were incorporated in an intumescent paint formulation, and characterized extensively in terms of thermal degradation behavior, intumescence thickness, and thermal insulation. Thermal microscopy images of charred foam development provided further information on the particular performance of each type of coating upon heating. The best foam expansion and heat protection results were obtained with the vinyl binders. Rheological measurements showed a complex evolution of the viscoelastic characteristics of the materials with temperature. As an example, the vinyl binders unexpectedly hardened significantly after thermal degradation. The values of storage moduli obtained at the onset of foam blowing (melamine decomposition) were used to explain different intumescence expansion behaviors. 相似文献
14.
Diana Tomás Joana Rodrigues Ana Varela Maria Manuela Veloso Wanda Viegas Manuela Silva 《International journal of molecular sciences》2016,17(2)
Genomic diversity of Portuguese accessions of Avena species—diploid A. strigosa and hexaploids A. sativa and A. sterilis—was evaluated through molecular and cytological analysis of 45S rDNA, and other repetitive sequences previously studied in cereal species—rye subtelomeric sequence (pSc200) and cereal centromeric sequence (CCS1). Additionally, retrotransposons and microsatellites targeting methodologies—IRAP (inter-retrotransposon amplified polymorphism) and REMAP (retrotransposon-microsatellite amplified polymorphism)—were performed. A very high homology was detected for ribosomal internal transcribed sequences (ITS1 and ITS2) between the species analyzed, although nucleolar organizing regions (NOR) fluorescent in situ hybridization (FISH) analysis revealed distinct number of Nor loci between diploid and hexaploid species. Moreover, morphological diversity, evidenced by FISH signals with different sizes, was observed between distinct accessions within each species. pSc200 sequences were for the first time isolated from Avena species but proven to be highly similar in all genotypes analyzed. The use of primers designed for CCS1 unraveled a sequence homologous to the Ty3/gypsy retrotransposon Cereba, that was mapped to centromeric regions of diploid and hexaploid species, being however restricted to the more related A and D haplomes. Retrotransposon-based methodologies disclosed species- and accessions-specific bands essential for the accurate discrimination of all genotypes studied. Centromeric, IRAP and REMAP profiles therefore allowed accurate assessment of inter and intraspecific variability, demonstrating the potential of these molecular markers on future oat breeding programs. 相似文献
15.
In paediatrics, the risks associated with ionising radiation should be a major concern, due to children's higher susceptibility to radiation effects. Measure entrance skin dose (ESD) in chest and pelvis X-ray projections and compare the results with the 'European guidelines on quality criteria for diagnostic radiographer images in paediatrics' in order to optimise radiological practice. ESD values were obtained using an ionisation chamber Diamentor M4 KDK (PTW) in 429 children, who underwent chest X-ray or pelvis X-ray in a Computed Radiography system. In the first phase of the study, data were collected according to protocols used in the department; in a second phase different tube voltage values were used according to patient weight. A third phase was carried out, only for chest X-ray, using the exposure parameters of phase 2, plus activating lateral ionisation chamber. Three paediatric radiologists blindly assessed image quality of chest X-ray, using a validated assessment available in the 'European guidelines on quality criteria for diagnostic radiographer images in paediatrics'. Considering all the patients submitted to chest X-ray, the average ESD was 0.22, 0.16 and 0.08 mGy, for phases 1, 2 and 3, respectively. For pelvis X-ray, the average ESD decreased from 1.18 mGy in phase 1 to 0.78 mGy in phase 2. Dose optimisation was achieved. ESD was reduced 63.6 and 33.9 % in chest and pelvis X-ray, respectively. 相似文献
16.
Joana S. Cristóv?o Sónia S. Leal Isabel Cardoso Cláudio M. Gomes 《International journal of molecular sciences》2013,14(9):19128-19145
Superoxide dismutase 1 (SOD1) aggregation is one of the pathological markers of amyotrophic lateral sclerosis (ALS), a fatal neurodegenerative disorder. The underlying molecular grounds of SOD1 pathologic aggregation remains obscure as mutations alone are not exclusively the cause for the formation of protein inclusions. Thus, other components in the cell environment likely play a key role in triggering SOD1 toxic aggregation in ALS. Recently, it was found that ALS patients present a specific altered metabolomic profile in the cerebrospinal fluid (CSF) where SOD1 is also present and potentially interacts with metabolites. Here we have investigated how some of these small molecules affect apoSOD1 structure and aggregation propensity. Our results show that as co-solvents, the tested small molecules do not affect apoSOD1 thermal stability but do influence its tertiary interactions and dynamics, as evidenced by combined biophysical analysis and proteolytic susceptibility. Moreover, these compounds influence apoSOD1 aggregation, decreasing nucleation time and promoting the formation of larger and less soluble aggregates, and in some cases polymeric assemblies apparently composed by spherical species resembling the soluble native protein. We conclude that some components of the ALS metabolome that shape the chemical environment in the CSF may influence apoSOD1 conformers and aggregation. 相似文献
17.
Helena Albano Catarina Pinho Daniela Leite Joana Barbosa Joana Silva Luísa Carneiro Rui Magalhães Tim Hogg Paula Teixeira 《Food Control》2009,20(8):764-770
This study was conducted to evaluate the ability of Pediococcus acidilactici HA-6111-2, a PA-1 bacteriocin-producing lactic acid bacterium (LAB), isolated from “Alheira” to inhibit a cocktail of Listeria innocua strains during production and shelf-life of these products. The bacteriocinogenic culture reduced the Listeria population below the detection limit (1.5log CFU/g) and had no effect on the growth of the natural LAB flora or on the pH. Pathogenic organisms were not detected in any sample. The presence of some virulence factors and antibiotic resistances of the strain to be used as a bioprotective culture were investigated. P. acidilactici HA-6111-2 did not produce any of the biogenic amines tested; no formation of biofilms was observed; more l(+)lactic acid was produced than its isomer d(?); no gelatinase, DNase or lipase activity was recorded; no structural genes for the haemolysin, enterococcal surface protein, hydrolytic compounds, aggregation protein and cell-wall adhesins were detected, no significant antibiotic resistances were found. P. acidilactici HA-6111-2 appears to have potential as a bioprotective culture during “Alheira” fermentation. Moreover, a trained panel considered the protected product to be sensorially acceptable. 相似文献
18.
João Gomes Joana L. Pereira Inês C. Rosa Pedro M. Saraiva Fernando Gonçalves Raquel Costa 《Journal of Great Lakes research》2014
The biofouling Asian clam Corbicula fluminea greatly affects freshwater-dependent facilities in Europe and North America, including in the Great Lakes region. As chlorination has become increasingly restricted, finding alternative control agents is a priority. Due to the species' epifaunal nature, the body of knowledge on Dreissena polymorpha is larger than that on the Asian clam, and there is a tendency to assume that mitigation methods should work similarly for both species. However, this generalisation is inaccurate, and the optimisation of Asian clam control relies on species-specific toxicological data. This paper reports information on the potential of three candidate biocides for C. fluminea control: (i) the cationic polydiallyldimethylammonium chloride (polyDADMAC), (ii) potassium chloride and (iii) aluminum sulphate. While these chemicals may be employed in a range of contexts, they are particularly suitable for the highly regulated drinking water industry. LT50 values ranging from 284.3 h, for polyDADMAC applied at 10 mg/L, to 855.1 h, for an aluminum sulphate concentration of 11 mg/L, were obtained. Ecotoxicological standard tests with Pseudokirchneriella subcapitata and Daphnia magna suggested that, amongst the three biocides, potassium chloride is the one representing lower potential environmental hazard (with, for example, 48 h-EC50 higher than 1 g/L for Daphnia immobilisation, which compares to values of 20.2 and 112.5 mg/L for polyDADMAC and aluminum sulphate, respectively). The three chemicals are promising control agents at dosages compatible with waterworks' operational requirements with polyDADMAC having the highest biocidal activity, but also posing more environmental risks. 相似文献
19.
Andr F. Silva Hugo Paisana Tnia Fernandes Joana Gis Armnio Serra Jorge F. J. Coelho Aníbal T. de Almeida Carmel Majidi Mahmoud Tavakoli 《Advanced Materials Technologies》2020,5(9)
A novel technique that permits, for the first time, fabrication of stretchable traces with linewidths as low as 20 µm and line‐spacing of 30 µm, based on simple coating and printing techniques, performed entirely at ambient condition, is demonstrated. By relying on existing inkjet printing technique, the proposed sinter‐free method is a step toward scalable fabrication of high‐resolution stretchable circuits, with application in logic gates, transparent conductors, and solar panels. This is accomplished by coating a layer of poly(vinyl alcohol) (PVA) over an elastic substrate, inkjet printing a circuit with silver nanoparticle (AgNP) ink, and then coating the printed circuit with a thin film of eutectic gallium‐indium‐tin (Galinstan) alloy. The Galinstan coating selectively wets to the printed AgNPs, resulting in highly conductive (6.65 × 106 S m−1) circuits that can withstand over 100% of strain with a modest gauge factor of ≈2.7. The process does not need thermal sintering, thanks to the Galinstan fusion with AgNPs, thus being compatible with heat‐sensitive substrates. The PVA coating has a critical role as a hydrophilic surface that absorbs the water‐based ink but resists wetting of the Galinstan. This method is demonstrated over a variety of substrates, including ultrasoft polyurethanes, ultra‐stretchable styrene–ethylene/butylynestyrene, and polyimide. 相似文献
20.
In recent years, cyber‐physical systems (CPS) have been widely used in different problem domains. The hardware and software components in a CPS are deeply intertwined at various levels of abstraction under changing contexts to achieve the desired goals. One way to manage this complexity is through the use of modular architecture that enables portions of a CPS to be upgraded, replaced, or fixed in a plug‐and‐play manner. In practice, nominally modular architectures depart from this ideal. Thus, there is a need to identify, measure, and understand the causes of these departures. Techniques have been developed to accomplish this for pure software systems, but can these techniques be adapted to infer hardware‐related modularity violations in a CPS? To investigate this question, we adapted methods from pure‐software systems and analyzed two open‐source CPS: OpenWrt and MD PnP. We found that the software architecture of these systems is well modularized. However, we were able to detect and measure modularity violations associated with hardware and other domain‐related concepts. Furthermore, software components related to hardware were more likely to change frequently compared to general software components. Together, these findings suggest that it is feasible to identify a subset of hardware‐driven modularity violations using techniques adapted from pure software systems. 相似文献