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991.
Sin Hang Lee Jessica S. Vigliotti Veronica S. Vigliotti William Jones David M. Shearer 《International journal of molecular sciences》2014,15(3):4284-4298
The diagnoses of Lyme disease based on clinical manifestations, serological findings and detection of infectious agents often contradict each other. We tested 52 blind-coded serum samples, including 20 pre-treatment and 12 post-treatment sera from clinically suspect Lyme disease patients, for the presence of residual Lyme disease infectious agents, using nested PCR amplification of a signature segment of the borrelial 16S ribosomal RNA gene for detection and direct DNA sequencing of the PCR amplicon for molecular validation. These archived sera were split from the samples drawn for the 2-tier serology tests performed by a CDC-approved laboratory, and are used as reference materials for evaluating new diagnostic reagents. Of the 12 post-treatment serum samples, we found DNA evidence of a novel borrelia of uncertain significance in one, which was also positive for the 2-tier serology test. The rest of the post-treatment sera and all 20 control sera were PCR-negative. Of the 20 pre-treatment sera from clinically suspect early Lyme disease patients, we found Borrelia miyamotoi in one which was 2-tier serology-negative, and a Borrelia burgdorferi in two—one negative and one positive for 2-tier serology. We conclude that a sensitive and reliable DNA-based test is needed to support the diagnosis of Lyme disease and Lyme disease-like borreliosis. 相似文献
992.
A. Catalano P. Ade Y. Atik A. Benoit E. Bréele J. J. Bock P. Camus M. Charra B. P. Crill N. Coron A. Coulais F.-X. Désert L. Fauvet Y. Giraud-Héraud O. Guillaudin W. Holmes W. C. Jones J.-M. Lamarre J. Macías-Pérez M. Martinez A. Miniussi A. Monfardini F. Pajot G. Patanchon A. Pelissier M. Piat J.-L. Puget C. Renault C. Rosset D. Santos A. Sauvé L. Spencer R. Sudiwala 《Journal of Low Temperature Physics》2014,176(5-6):773-786
The Planck High Frequency Instrument (HFI) has been surveying the sky continuously from the second Lagrangian point (L2) between August 2009 and January 2012. It operates with 52 high impedance bolometers cooled at 100 mK in a range of frequency between 100 GHz and 1 THz with unprecedented sensitivity, but strong coupling with cosmic radiation. At L2, the particle flux is about 5 \(\hbox {cm}^{-2}\,\hbox {s}^{-1}\) and is dominated by protons incident on the spacecraft. Protons with an energy above 40 MeV can penetrate the focal plane unit box causing two different effects: glitches in the raw data from direct interaction of cosmic rays with detectors (producing a data loss of about 15 % at the end of the mission) and thermal drifts in the bolometer plate at 100 mK adding non-Gaussian noise at frequencies below 0.1 Hz. The HFI consortium has made strong efforts in order to correct for this effect on the time ordered data and final Planck maps. This work intends to give a view of the physical explanation of the glitches observed in the HFI instrument in-flight. To reach this goal, we performed several ground-based experiments using protons and \(\alpha \) particles to test the impact of particles on the HFI spare bolometers with a better control of the environmental conditions with respect to the in-flight data. We have shown that the dominant part of glitches observed in the data comes from the impact of cosmic rays in the silicon die frame supporting the micro-machined bolometric detectors propagating energy mainly by ballistic phonons and by thermal diffusion. The implications of these results for future satellite missions will be discussed. 相似文献
993.
Kristiina Laine Kristoffer Segerholm Magnus Wålinder Lauri Rautkari Graham Ormondroyd Mark Hughes Dennis Jones 《Journal of Materials Science》2014,49(5):2027-2034
Scots pine (Pinus sylvestris L.) wood was surface densified in its radial direction in an open press with one heated plate to obtain a higher density on the wood surface whilst retaining the overall thickness of the sample. This study investigated the effect of temperature (100, 150 and 200 °C) and press closing speed (5, 10 and 30 mm/min, giving closing times of 60, 30 and 10 s, respectively) on the micromorphology of the cell-wall, as well as changes occurring during set-recovery of the densified wood. The micromorphology was analysed using scanning electron microscopy (SEM) combined with a sample preparation technique based on ultraviolet-excimer laser ablation. Furthermore, the density profiles of the samples were measured. Low press temperature (100 °C) and short closing time (10 s) resulted in more deformation through the whole thickness, whilst increasing the temperature (150 and 200 °C) and prolonging the closing time (30 and 60 s) enabled more targeted deformation closer to the heated plate. The deformation occurred in the earlywood regions as curling and twisting of the radial cell-walls, however, no apparent cell-wall disruption or internal fracture was observed, even at low temperatures and fast press closing speed, nor after soaking and drying of the samples. In the SEM-analysis after soaking and drying, it was noticed that the cells did not completely recover their original form. Thus, part of the deformation was considered permanent perhaps due to viscoelastic flow and plastic deformation of the cell-wall components. 相似文献
994.
C. M. Groh M. E. Hubbard P. F. Jones P. M. Loadman N. Periasamy B. D. Sleeman S. W. Smye C. J. Twelves R. M. Phillips 《Journal of the Royal Society Interface》2014,11(94)
The ability to predict how far a drug will penetrate into the tumour microenvironment within its pharmacokinetic (PK) lifespan would provide valuable information about therapeutic response. As the PK profile is directly related to the route and schedule of drug administration, an in silico tool that can predict the drug administration schedule that results in optimal drug delivery to tumours would streamline clinical trial design. This paper investigates the application of mathematical and computational modelling techniques to help improve our understanding of the fundamental mechanisms underlying drug delivery, and compares the performance of a simple model with more complex approaches. Three models of drug transport are developed, all based on the same drug binding model and parametrized by bespoke in vitro experiments. Their predictions, compared for a ‘tumour cord’ geometry, are qualitatively and quantitatively similar. We assess the effect of varying the PK profile of the supplied drug, and the binding affinity of the drug to tumour cells, on the concentration of drug reaching cells and the accumulated exposure of cells to drug at arbitrary distances from a supplying blood vessel. This is a contribution towards developing a useful drug transport modelling tool for informing strategies for the treatment of tumour cells which are ‘pharmacokinetically resistant’ to chemotherapeutic strategies. 相似文献
995.
The combustion reactivity of rice husk from New South Wales,ustralia was measured by heating cubical baskets in controlled environment and monitoring the centre temperature.Frank-Kamenetskii‘s ignition theory was used to calculate the activation energy,Results agreed well with tests on other cellulosic materials,Numerical simulations of temperature changes were carried out,using a special technique to deal with non-linearities in the heat conduction equation.The numerical Results indicate that the time factor should be taken into account when evaluating self-combustion risks. 相似文献
996.
997.
Klánová J Diamond M Jones K Lammel G Lohmann R Pirrone N Scheringer M Balducci C Bidleman T Bláha K Bláha L Booij K Bouwman H Breivik K Eckhardt S Fiedler H Garrigues P Harner T Holoubek I Hung H MacLeod M Magulova K Mosca S Pistocchi A Simonich S Smedes F Stephanou E Sweetman A Sebková K Venier M Vighi M Vrana B Wania F Weber R Weiss P 《Environmental science & technology》2011,45(18):7617-7619
998.
Castorina R Bradman A Sjödin A Fenster L Jones RS Harley KG Eisen EA Eskenazi B 《Environmental science & technology》2011,45(15):6553-6560
We measured levels of 10 polybrominated diphenyl ether (PBDE) congeners in serum collected during pregnancy and at delivery from 416 pregnant, predominantly immigrant, women living in Monterey County, CA. The most frequently detected congeners were BDE-47, -99, -100, and -153, all components of the penta mixture, detected in >97% of samples. We used multivariable regression models to examine factors associated with exposure to individual PBDE congeners as well as their total summed concentration (ng/g lipid). Prenatal and delivery total PBDE levels were correlated between sampling times (n = 21; Pearson r = 0.99, p < 0.001). In multivariable models, total PBDE levels increased significantly with time residing in the U.S. (p < 0.001) and among women with ≥3 pieces of stuffed furniture in their homes (p < 0.05). Women's total PBDE levels increased 4.0% (95% CI = 2.8, 5.3) for each additional year residing in the U.S., after adjustment for prepregnancy BMI, weight gain during pregnancy, and SES. Having ≥3 pieces of stuffed furniture in the home was associated with a 26.8% (95% CI = 2.0, 57.5) increase in women's serum PBDE levels. Findings suggest PBDE indoor contamination in California homes is contributing to human exposures in a population of recent immigrants. 相似文献
999.
1000.
There is no suitable model for predicting thermal inactivation kinetics of Salmonella spp. for many types of liquid egg products, including salted liquid egg yolk, for use in updating U.S. Department of Agriculture (USDA) pasteurization guidelines. This is because, in part, of the variations in Salmonella strains and the changes in the processing of liquid egg products over the past 40 years. The objectives of the present study were to determine the thermal inactivation kinetics and to create a general thermal inactivation kinetics model that can be used for estimating log reductions of salmonellae in 10% salted liquid egg yolk for temperatures between 62.2 and 69°C. This model can be used by processors to help ensure adequate pasteurization. This was accomplished by studying the inactivation kinetics of a three-strain composite of heat-resistant Salmonella serovars Enteritidis and Oranienburg, inoculated into commercially processed 10% salted liquid egg yolk. The survival curves were convex, with asymptotic D-values. From these curves, a general model was developed to predict log reductions for given times at specified temperatures. For example, at a temperature of 67.3°C (153.1°F) for 3.5 min, our model predicts a 5-log reduction would be obtained, whereas with the current USDA minimum required pasteurization regimen (63.33°C [146°F] for 3.5 min), our model predicts that a reduction of only 2.7 log would be obtained. The results of this study provide information that can be used by processors to aid in producing safe, pasteurized egg yolk products, and for satisfying USDA pasteurization performance standards and developing industry guidance. 相似文献