Response surface optimisation of germination conditions to improve the accumulation of bioactive compounds and the antioxidant activity in quinoa

Germination has been proposed as an economic approach to improve the content of bioactive compounds in pseudocereals. In this work, response surface methodology (RSM) was employed to investigate the impact of germination conditions on the phytochemical content and antioxidant activity of quinoa. The use of desirability methodology showed that the optimum conditions to maximise the content of total phenolic content (TPC) and antioxidant activity in sprouted quinoa were 20 °C for 42 h. Sprouts produced under these conditions exhibited increases of 80% and 30% in TPC and antioxidant activity, respectively, compared to un‐germinated seeds, and contained high γ‐aminobutyric acid (GABA) concentration. The nonsignificant lack‐of‐fit and high determination coefficients obtained confirmed the suitability of the predictive models developed for TPC and antioxidant activity, whilst the one obtained for GABA was not significant (R² < 0.75) within the conditions studied. Sprouting under optimum conditions enhanced the content of both flavonoid and nonflavonoid compounds, being the increase in flavonoids more pronounced. Kaempferol‐O‐dirhamnosyl‐galactopyranose and quercetin‐O‐glucuronide were the compounds that experienced the most noticeable increase in quinoa after germination. In conclusion, this study provides useful information on the optimum germination conditions to improve the levels of health‐promoting compounds in quinoa.     

Metabolic role of glutamine and its importance in nutritional therapy

The advances in the field of nutritional support have made certain nutrients very relevant, which, although they have been known for a long time, at present represent an important chapter in nutrition, entering into what is known as "nutritional pharmacology". Among these nutrients is glutamine, an amino acid classified as non-essential, but which in certain circumstances may become to be considered as an "essential nutrient". In the present review, a review is made of its metabolic role, synthesis and degradation, metabolic routes and functions under normal conditions as well as under critical conditions. It is known that glutamine stimulates the synthesis and inhibits the degradation of proteins, it is an important vehicle for the transport of nitrogen and carbon within the tissues, it stimulates the synthesis of hepatic glycogen, it is an energy source for cell division, for the growth of different cells of rapid replication, such as enterocytes, colonocytes, and fibroblasts, as well as for other cells of the immune system, such as lymphocytes and macrophages. Thus its role in the maintenance of structure, in metabolism and function of the intestinal mucosa, and in dysfunctions of the immune system. In parenteral nutrition, at present there are no preparations which include it, given the stability problems which it presents, although attempts have been made to resolve this, using different possibilities, such as di-peptides. However, in enteral nutrition, the diets tend to include it, although in a small proportion. Nevertheless, having recognized its beneficial role in a certain type of patients, at present there are diets which contain glutamine in higher doses, with the object of attempting to cover the increased demands of glutamine which shall arise in these situations. The inclusion of glutamine in nutritional therapy is supported by multiple studies which reflect the beneficial effect of this nutrient, both in enteral nutrition as in parenteral nutrition.     

Synthesis of a porphyrin–C60 dyad for potential use in solar energy conversion

A convenient procedure for the synthesis of a new porphyrin–C₆₀ dyad via the reaction between 5-(4-aminophenyl)-10,15,20-tris(4-methoxyphenyl) porphyrin and a C₆₀ carboxylic acid is described. The results of preliminary photoelectrochemical studies on nanostructured SnO₂ electrodes coated with the new dyad suggest that the resultant substrate is suitable for solar energy conversion devices.     

Procedures for Determining Maximum Emitter Discharge in Subsurface Drip Irrigation

One problem associated with subsurface drip irrigation (SDI) is the reduction in discharge resulting from soil-water back-pressure at the emitter outlets. An experimental setup was made to measure emitter discharge and pressure at the emitter outlet in different soils. Experiments were carried out with 2–24??L/h noncompensating and compensating emitters, operating at a constant lateral pressure of 10?m. Emitter discharge was reduced to a range of 2–10% for noncompensating models and to less than 1% for compensating models. Soil pressure ranged from 0.15–2.07?m. Laboratory conditions were simulated with HYDRUS-2D/3D. Experimental values of discharge and soil pressure showed good agreement with estimated values. Finally, maximum emitter discharge to limit the decrease of discharge was determined for an operating pressure of 10?m. For a 10% decrease, considering a constant radius of the spherical cavity in the soil, maximum emitter discharge was 2.35??L/h for loamy soil and 12.44??L/h for sandy soil for noncompensating emitters, and 10.73 and 54.51??L/h, respectively for compensating emitters. These values increased when considering a cavity radius variable with emitter discharge.     

Probability of Pressure Deficit in On-Demand Branched Networks and Incorporation into Design Decisions

This technical paper presents analytical expressions to estimate the probability function of head losses in any path of an on-demand branched irrigation network. They are developed for estimating the probability of pressure deficit of a given magnitude at any hydrant. They are also useful for examining the probability of a power deficit at a pumping station designed to guarantee service to a hydrant, as well as the head characteristic curve of the distribution system linked to a definite probability. All this quantified information is useful for decision making on network design and performance. The probabilities calculated with the developed expressions can be taken as complementary or alternative concepts to Clément’s classical design flow method, which is taken here as a benchmark for comparisons. Illustrative examples of network designs are presented to validate the proposed expressions. The least cost design solutions using Clément’s design flows are compared with design solutions here obtained to get the same probability of pressure deficit at the most unfavorable hydrants. The new solutions are less expensive because the flow constraint can be avoided.     

Determining Minor Head Losses in Drip Irrigation Laterals. I: Methodology

Minor head losses at emitter insertions along drip laterals were predicted by a derivation of Bélanger’s theorem and analyzed by the classic formula that includes a friction coefficient K multiplied by a kinetic energy term. A relationship was established for K as a function of some emitter geometric characteristics. These take into account the flow expansion behind the reduction of the cross-sectional area of the pipe due to obstruction by the emitter. Flow constrictions at emitter insertions were estimated by analogy with contraction produced by water jets discharging through orifices. An experimental procedure was also developed to determine minor losses in situ, in the laboratory or in the field. An approach is suggested to calculate either K or the emitter equivalent length le as a function of lateral head losses, inlet head, and flow rate. Internal diameter and length of lateral, emitter spacing, emitter discharge equation, and water viscosity must be known. Approximate analytical relations to study flow in laterals were developed. They may be used to design and evaluate drip irrigation units. Analytical and experimental procedures are validated in the companion paper by Juana et al.