Extracting Refined Rules from Knowledge-Based Neural Networks

Neural networks, despite their empirically proven abilities, have been little used for the refinement of existing knowledge because this task requires a three-step process. First, knowledge must be inserted into a neural network. Second, the network must be refined. Third, the refined knowledge must be extracted from the network. We have previously described a method for the first step of this process. Standard neural learning techniques can accomplish the second step. In this article, we propose and empirically evaluate a method for the final, and possibly most difficult, step. Our method efficiently extracts symbolic rules from trained neural networks. The four major results of empirical tests of this method are that the extracted rules 1) closely reproduce the accuracy of the network from which they are extracted; 2) are superior to the rules produced by methods that directly refine symbolic rules; 3) are superior to those produced by previous techniques for extracting rules from trained neural networks; and 4) are human comprehensible. Thus, this method demonstrates that neural networks can be used to effectively refine symbolic knowledge. Moreover, the rule-extraction technique developed herein contributes to the understanding of how symbolic and connectionist approaches to artificial intelligence can be profitably integrated.     

NOVeLLA: A Multi-Modal Electronic-Book Reader With Visual and Auditory Interfaces

While significant growth in the popularity of electronic books seems likely over the next few years, current systems and near-term proposals from major vendors have shown little foresight in leveraging the equally inevitable increase in processing power available to such systems. In this note we describe a prototype electronic-book reader, NOVeLLA, whose design takes as its starting point the assumption that computing power equivalent to today's high-end desktop systems will be available in hand-held formats within just a few years. It proposes interesting ways in which that power might be utilized, in particular by delivering a powerful speech-based user interface. A secondary goal of the project was to implement the system using off-the-shelf software technologies and Application Programming Interfaces (APIs) available freely or cheaply today, testing whether those technologies and related standards were adequate to the task. Finally, NOVeLLA is the first generally available reader compatible with the unencoded version of the new Open Ebook Document Structure.     

CARBON FIBER-POLYANILINE COMPOSITES: KINETICS OF ELECTRODEPOSITION OF POLYANILINE ONTO CARBON FIBERS BY CYCLIC VOLTAMMETRY

Homogeneous and uniform coatings of polyaniline were successfully deposited on carbon fibers by an aqueous electrodeposition technique using p-toluene sulfonic acid as the electrolyte. Electrochemical deposition of aniline was carried out by cyclic voltammetry in the potential range of -0.2 V to 1.0 V versus saturated calomel electrode (SCE). The electrochemical deposition parameters such as the number of cycles, scan rate (SR), initial monomer ([M]), and electrolyte concentration ([E]) were systematically varied. The amount of composite coatings on carbon fibers was dependent on the electrochemical deposition parameters. From a weight gain analysis, the rate of the reactions (R p ) was calculated. As the aniline concentration was increased up to 0.35 M and electrolyte concentration up to 0.5 M, the deposition rate also increased, whereas an increase in scan rate decreased the deposition rate. Kinetic analysis showed that the rate equation for the p-toluene sulfonic acid system is R p ∝SR -1.25 [M] 0.73 [E] 0.95 . IR spectra also showed an increase in the deposition of polyaniline coatings on carbon fibers with a decrease in the scan rate and an increase in both monomer and electrolyte concentration. The ratio of two oxidation states of polyaniline obtained during electrodeposition, namely emeraldine and pernigraniline, can be varied by changing the electrochemical deposition parameters.     

Synthesis and characterisation of thermo-sensitive terpolymer hydrogels for drug delivery applications

In this study, thermo-sensitive terpolymer hydrogels based on N-tert-butylacrylamide (NtBAAm), N-isopropylacrylamide (NIPAAm) and N-vinyl pyrrolidone (NVP) were successfully photopolymerised and characterised. 1-hydroxy-cyclohexylphenylketone (Irgacure 184) and 2-hydroxy-2-methyl-1-phenyl-propanone (Irgacure 2959) were used as light-sensitive initiators to initiate the reactions. Chemical structures of the hydrogels were confirmed using Fourier transform infrared (FTIR) and nuclear magnetic resonance (NMR) spectroscopy. The hydrogels were also characterised using modulated differential scanning calorimetry (MDSC) for their glass transition and phase transition temperatures. A single glass transition temperature (T _g ) was observed, further confirming successful formation of a terpolymer. The hydrogels were thermo-responsive, exhibiting a decrease in lower critical solution temperature (LCST) as the NtBAAm weight ratio was increased. Pulsatile swelling studies indicated that the hydrogels had thermo-reversible properties and the swelling properties were dependent on test temperature, monomer feed ratios and crosslinker content. The proposed hydrogel system could find applications in a broader field of gel/drug interaction, for the development of controlled release and targeted delivery devices.     

Mineralizable soil nitrogen and labile soil organic matter in diverse long-term cropping systems

Sustainable soil fertility management depends on long-term integrated strategies that build and maintain soil organic matter and mineralizable soil N levels. These strategies increase the portion of crop N needs met by soil N and reduce dependence on external N inputs required for crop production. To better understand the impact of management on soil N dynamics, we conducted field and laboratory research on five diverse management systems at a long-term study in Maryland, the USDA- Agricultural Research Service Beltsville Farming Systems Project (FSP). The FSP is comprised of a conventional no-till corn (Zea mays L.)–soybean (Glycine max L.)–wheat (Triticum aestivum L.)/double-crop soybean rotation (NT), a conventional chisel-till corn–soybean–wheat/soybean rotation (CT), a 2 year organic corn–soybean rotation (Org2), a 3 year organic corn–soybean–wheat rotation (Org3), and a 6 year organic corn–soybean–wheat–alfalfa (Medicago sativa L.) (3 years) rotation (Org6). We found that total potentially mineralizable N in organic systems (average 315 kg N ha⁻¹) was significantly greater than the conventional systems (average 235 kg N ha⁻¹). Particulate organic matter (POM)–C and –N also tended to be greater in organic than conventional cropping systems. Average corn yield and N uptake from unamended (minus N) field microplots were 40 and 48%, respectively, greater in organic than conventional grain cropping systems. Among the three organic systems, all measures of N availability tended to increase with increasing frequency of manure application and crop rotation length (Org2 < Org3 ≤ Org6) while most measures were similar between NT and CT. Our results demonstrate that organic soil fertility management increases soil N availability by increasing labile soil organic matter. Relatively high levels of mineralizable soil N must be considered when developing soil fertility management plans for organic systems.     

Polyimide-b-polysiloxane Copolymers: Synthesis and Properties

This review discusses the synthesis, structure and properties of polyimide-b-polysiloxane copolymers. Polysiloxane modified polyimides has become an important class of materials over the past 20 years because of their ease of processing and interesting properties. Although polyimides are widely used in engineering applications because of their outstanding mechanical properties and thermal stability, their processability, toughness, and adhesion can be significantly enhanced by incorporation of polysiloxanes. The synthesis of polyimide-b-polysiloxane copolymers is performed by a one-step copolymerization method or a two-step method, resulting in a wide range structures and thermo-mechanical properties. The initial modulus and thermal stability of polyimide-b-linear polysiloxane copolymers can be further improved by copolymerization with ladder-like polysiloxanes which possess outstanding thermal stability and mechanical properties. The study of synthesis and properties of polyimide-b-polysiloxane copolymers is essential because it provides a useful insight into the processing-structure–property relationship.     

Identification of Recurrent Mutations in the microRNA-Binding Sites of B-Cell Lymphoma-Associated Genes in Follicular Lymphoma

Follicular lymphoma (FL) is a common indolent B-cell lymphoma that can transform into the more aggressive transformed FL (tFL). However, the molecular process driving this transformation is uncertain. In this work, we aimed to identify microRNA (miRNA)-binding sites recurrently mutated in follicular lymphoma patients, as well as in transformed FL patients. Using whole-genome sequencing data from FL tumors, we discovered 544 mutations located in bioinformatically predicted microRNA-binding sites. We then studied these specific regions using targeted sequencing in a cohort of 55 FL patients, found 16 recurrent mutations, and identified a further 69 variants. After filtering for QC, we identified 21 genes with mutated miRNA-binding sites that were also enriched for B-cell-associated genes by Gene Ontology. Over 40% of mutations identified in these genes were present exclusively in tFL patients. We validated the predicted miRNA-binding sites of five of the genes by luciferase assay and demonstrated that the identified mutations in BCL2 and EZH2 genes impaired the binding efficiency of miR-5008 and miR-144 and regulated the endogenous levels of messenger RNA (mRNA).     

Crack propagation behavior and toughness of V‐notched polyethylene terephthalate injection moldings

The role of the skin and core regions in controlling the effects of V‐notches, on the fracture behavior of PET injection‐moldings, was correlated with their tensile and impact properties. Investigations revealed that there were three distinct fracture behaviors: ductile, semiductile, and brittle fracture transitions. The notch sensitivity factor for strength (K_S) in the ductile and semiductile transitions indicates that the fracture strength was not sensitive to ≤1.5 mm deep notches, which is considered the skin region. The introduction of core‐deep notches (>1.5 mm) resulted in a rapid increase in K_S. On the other hand, the notch sensitivity factor for energy (K_T) shows that the fracture energy was not sensitive at ≤0.5 mm deep notches. However, K_T increased drastically when notches >0.5 mm deep were introduced. The development of an anisotropic skin‐core structure in injection moldings is well acknowledged. This is revealed in a constant fracture behavior between 0.6 and 1.0 mm deep notches. Notably, there was a drastic change in the fracture pattern from ductile to semiductile at a critical 0.6 mm deep notch. The specimens experienced a mixed fracture behavior at 1.5 mm deep notch, which marks a transitional fracture pattern at the interface between the skin and core regions. Lastly, a constant fracture behavior was observed at notch depths ≥1.5 mm. Results show that crack opening, in the samples that had semiductile fracture, was a postnecking phenomenon. Before shear yielding, two shear lines that intersected at 45° were seen to originate from the crack root when a 1.2 kN load was applied. Conversely, crack opening and failure occurred simultaneously in brittle fractures. It is obvious that V‐notches provided a gradual transition in fracture behavior from the skin to the core regions, which confirms that the fracture behavior of PET injection moldings can be dependent on the skin and core structure. © 2009 Wiley Periodicals, Inc. J Appl Polym Sci, 2010     

Dehydroepiandrosterone alters lipid profiles in Zucker rats

High free fatty acid (FFA) levels are common in obesity and in diseases such as diabetes that are associated with the obese state. Dehydroepiandrosterone (DHEA) decreases dietary fat consumption, body fat content, and insulin levels in the obese Zucker rat (ZR), a genetic model of human youth-onset obesity and type 2 diabetes mellitus. This study was conducted to investigate the effects of DHEA on lean and obese ZR serum, adipose, and hepatic tissue fatty acid (FA) profiles and serum FFA levels. Because DHEA is known to decrease fat consumption and body fat, we postulate that DHEA may also alter FA profiles and FFA levels of the obese ZR such that they more closely resemble the profiles and levels of their lean siblings. In this study there was a DHEA and a pair-fed (PF) group (n=6) for 12 lean and 12 obese ZR. The diet of the treatment groups was supplemented with 0.6% DHEA, and PF groups were given the same average calories consumed by their corresponding DHEA group for 30 d. Fasted animals were sacrificed, and FA profiles and FFA levels were measured. Serum FFA levels were higher in obese (∼1 mmol/L) compared to lean rats (∼0.6 mmol/L). After 30 d of DHEA treatment, FFA levels were lower (P<0.05) in both lean and obese groups. Although several significant differences in FA profile of serum, hepatic, and adipose lipid components were observed between lean and obese ZR, DHEA-related changes were only observed in the serum phospholipid (PL) and liver PL and triglyceride fractions. The slight but significant decrease in serum FFA levels may be reflected by changes in serum PL FA profiles. Specific hepatic FA profile alterations may be related to DHEA's known effects in inducing hepatic peroxisomes. We speculate that such FA changes may give insight into a mechanism for the action of DHEA.