Comparison of phase unwrapping algorithms for living cell’s interference pattern

A multiphase microscopic interference system is designed to measure the height of cell which is important to the research of collective cell migration in physiology and medicine. This sys- tem can quantitatively measure cell height across a living monolayer without knowing the refractive index of cells. For the interference pattern, because the phases are all wrapped between - π to π, it is necessary to get the real phase through phase unwrapping,a method to restore the wrapped phase data of the object by using numerical calculations. Three representative algorithms are selected to unwrap the interference pattern of ceils： branch-cut method, quality-guided method and network method. Although each of them can restore the phase, their performances are obviously different. We compare these methods and find that branch-cut method needs the smallest execution time and can obtain good unwrapped patterns when noises are not serious.     

手性液相色谱法直接定量乳粉中RRR-α-生育酚

目的 建立手性液相色谱法直接测定乳粉中RRR-α-生育酚的检测方法.方法 样品经氢氧化钾皂化,石油醚-异丙醚萃取.经小粒径纤维素衍生物型手性柱Daicel Chiralcel OD-3(2.1 mm×150 mm,3μm)直接分离,采用无水乙醇-正己烷(5:995,V:V)(A)和三乙胺-正己烷(5:995,V:V)(...     

HOLLOW FIBER ULTRAFILTRATION OF CALF SERUM AND ALBUMIN IN THE PREGEL UNIFORM-WALL-FLUX REGION

Ultrafiltration of calf serum and bovine serum albumin (BSA) in the pregel region has been studied in Cuprophan hollow-fiber filters of various lengths and number of fibers, and at several axial flow velocities. Graphs of ultrafiltration velocities versus transmembrane pressure show a surprising independence from fiber length and axial flow velocity. Results are correlated by osmotic-pressure data and an available boundary layer theory derived originally for uniform-wall-flux processes in the far-downstream (asymptotic) region.     

Misting Affects Market Quality and Enzyme Activity of Broccoli During Retail Storage

Vitamin C, enzyme activities, color and texture changed in misted and nonmisted broccoli at 0, 24, 48 and 72 hr intervals during display cabinet storage (18°C). Reduced ascorbic acid (RAA), ascorbate oxidase and peroxidase activities, total chlorophyll extractions, Hunter-lab color and Instron texture measurements were followed. Misting significantly enhanced RAA (p=0.0001), and retention of chlorophyll (p=0.0001), and green color as measured by hue angle (p=0.0021) during 72 hr storage. RAA degradation in misted and nonmisted broccoli and total chlorophyll degradation in nonmisted samples followed first order kinetics. Peroxidase activity was significantly greater in both the nonmisted broccoli floret/stem (p = 0.0221) and stalk tissue (p=0.0079). No significant differences were found between misted and nonmisted broccoli for ascorbate oxidase activity (p = 0.9426) or Instron shear values (p = 0.3652).