Purification,characterisation and application of α‐l‐rhamnosidase from Penicillium citrinum MTCC‐8897

An α‐l ‐rhamnosidase secreted by Penicillium citrinum MTCC‐8897 has been purified to homogeneity from the culture filtrate of the fungal strain using ammonium sulphate precipitation and cation‐exchange chromatography on carboxymethyl cellulose. The sodium dodecyl sulphate/polyacrylamide gel electrophoresis analysis of the purified enzyme gave a single protein band corresponding to the molecular mass 51.0 kDa. The native polyacrylamide gel electrophoresis also gave a single protein band confirming the enzyme purity. The K_m and V_max values of the enzyme for p‐nitrophenyl α‐l ‐rhamnopyranoside were 0.36 mm and 22.54 μmole min^?1 mg^?1, respectively, and k_cat value was 17.1 s^?1 giving k_cat/K_m value of 4.75 × 10⁴ m ^?1 s^?1. The pH and temperature optima of the enzyme were 7.0 and 60 °C, respectively. The purified enzyme liberated l ‐rhamnose from naringin, rutin, hesperidin and wine, indicating that it has biotechnological application potential for the preparation of l ‐rhamnose and other pharmaceutically important compounds from natural glycosides containing terminal α‐l ‐rhamnose and also in the enhancement of wine aroma.     

VO2 nanostructures based chemiresistors for low power energy consumption hydrogen sensing

Mott-type VO₂ oxide nanobelts are demonstrated to be effective hydrogen gas sensors at room temperature. These nanobelts, synthesized by hydrothermal process and exhibiting the VO₂ (A) crystallographic phase, display room temperature H₂ sensitivity as low as 0.17 ppm. The nanobelts (ultralong belt-like) nanostructures could be an ideal system for fully understanding dimensionally confined transport phenomena in functional oxides and for building functional devices based on individual nanobelts.     

圣摩尼卡海岸酒店

"这里是我们生活和工作的地方,我们希望与我们的客人分享这里的一切,告诉他们是什么令圣莫尼卡成为一个奇妙的旅行胜地。"首席执行官SteveFarzam这样说,"我们相信,海岸酒店的先进经验和环保设施将创造出与众不同的新体验。"     

Assessment of diverse image encryption mechanisms under prevalent invasion

Multimedia Tools and Applications - Image encryption mechanisms provide confidentiality and concealment of information (image) in transmission over the alleyway, susceptible to prevalent invasions....     

Flow‐based electromagnetic‐type energy harvester using microplanar coil for IoT sensors application

Battery is the sole power source for Internet of thing (IoT) sensors. Due to limited shelf life, the batteries are required to be replaced intermittently. This periodic replacement of batteries is inflated in terms of both logistics and time. This article illustrates conceptual design, development, and characterization of a flow‐based electromagnetic‐type energy harvester (F‐EH) using microplanar coil for IoT sensors application. The F‐EH converts hydro energy into useful electrical energy utilizing electromagnetic transduction mechanism. The microfabrication and macrofabrication techniques adopted to manufacture harvester's components are presented. The F‐EH has been successfully characterized by laboratory scale experimental flow test loop commissioned for this work. Experimentation with associated uncertainty analysis prevails that at a matching impedance, the F‐EH can generate a 686 μW of maximum power at an operating flow rate of 12 L/min with an uncertainty of 8.1%.     

Fabrication of Mn–ZnO photoanodes for photoelectrochemical water splitting applications

Journal of Materials Science: Materials in Electronics - A photoelectrochemical (PEC) water splitting ability of pure ZnO and manganese-incorporated ZnO thin films fabricated via a simple...     

Cognitive Mapping and Planning for Visual Navigation

International Journal of Computer Vision - We introduce a neural architecture for navigation in novel environments. Our proposed architecture learns to map from first-person views and plans a...     

Novel anhydrous composite membranes based on sulfonated poly (ether ketone) and aprotic ionic liquids for high temperature polymer electrolyte membranes for fuel cell applications

Novel composite membranes were prepared using imidazolium type aprotic ionic liquids and sulfonated poly (ether ketone) (SPEK) as polymer matrix by solution casting process. All the prepared membranes were characterized for their thermal stability, mechanical properties, ion exchange capacity, proton conductivity and leaching out of ionic liquids in presence of water. Ionic liquid based membranes were more flexible than neat SPEK membrane due to the plasticization effect of ionic liquids. The interactions and compatibility occurring among components were investigated by vibration spectroscopy (FTIR ATR) and scanning electron microscopy respectively. The thermal stability of composite membranes was higher than unmodified membranes. The ion conductivity of composite membranes under anhydrous conditions was found to be dependent on temperature, type and concentration of ionic liquid in SPEK matrix. Ion conductivities of composite membranes under anhydrous condition were found to be up to two orders (∼100 times) higher than neat SPEK membrane and it was found to be ∼5 mS/cm at 140 °C for SPEK/OTf-70. These composite membranes can be successfully operated at temperatures ranging from 40 °C to 140 °C under anhydrous conditions.     

Modification of glass ionomer cement by incorporating hydroxyapatite-silica nano-powder composite: Sol–gel synthesis and characterization

A nanohydroxyapatite–silica powder was synthesized using an ethanol based sol–gel technique. The synthesized powder was incorporated into commercial glass ionomer powder (Fuji II GC) and characterized using FTIR, ²⁹Si CP/MAS NMR, EDX and XRD spectroscopy. ²⁹Si CP/MAS NMR results showed the presence of higher degree of cross-linking of silyl species between silica and GIC, which makes the Nano-HA–Silica–GIC composite much stronger. High-resolution transmission electron microscopy (TEM) and scanning electron microscopy (TEM) was employed to investigate the morphology of the synthesized powder. Results revealed that higher content of nanosilica produced a denser and stronger GIC. Thus, the application of nanohydroxyapatite–silica–GIC with improved properties are envisioned to be of great clinical importance, especially in stress bearing areas.