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Bart van Dam Hui Nie Bo Ju Emanuele Marino Jos M. J. Paulusse Peter Schall Minjie Li Katerina Dohnalová 《Small (Weinheim an der Bergstrasse, Germany)》2017,13(48)
Carbon dots (CDs) are carbon‐based fluorescent nanoparticles that can exhibit excitation‐dependent photoluminescence (PL) “tunable” throughout the entire visible range, interesting for optoelectronic and imaging applications. The mechanism underlying this tunable emission remains largely debated, most prominently being ascribed to dot‐to‐dot variations that ultimately lead to excitation‐dependent ensemble properties. Here, single‐dot spectroscopy is used to elucidate the origin of the excitation‐dependent PL of CDs. It is demonstrated that already single CDs exhibit excitation‐dependent PL spectra, similar to those of the CD ensemble. The single dots, produced by a facile one‐step synthesis from chloroform and diethylamine, exhibit emission spectra with several characteristic peaks differing in emission peak position and spectral width and shape, indicating the presence of distinct emission sites on the CDs. Based on previous work, these emission sites are related to the sp2 subregions in the carbon core, as well as the functional groups on the surface. These results confirm that it is possible to integrate and engineer different types of electronic transitions at the nanoscale on a single CD, making these CDs even more versatile than organic dyes or inorganic quantum dots and opening up new routes toward light‐emission engineering. 相似文献
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Katerina Girginova 《Digital Creativity》2017,28(1):8-23
ABSTRACTThis paper analyzes #savethesurprise, the hugely successful social media campaign launched prior to the London 2012 Olympic Games. In brief, #savethesurprise helped to persuade over 100,000 audience members not to divulge on social media any of what they saw during the two, live dress rehearsals of the Games’ opening ceremony; this ceremony then went on to become a part of the most watched television event in history. To explore how and why the campaign worked, this paper introduces the concept of ‘architectures of participation’ (AoP). AoP are socio-technical structures and practices that shape the ways in which people and organizations co-construct events and realities via communication. By applying this concept to the #savethesurprise campaign, this paper presents a participatory architecture undergirded by strong legal, technical and social layers. Furthermore, a sense of creative citizenship and fair play emerge as key audience motivators. 相似文献
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Changes in fish macrophages (Macs) are useful indicators of environmental pressures, but responses due to chemical and nonchemical stresses may be confounded by natural sources of variability. These may include sex and gonadal stage. In this study, we start addressing the following question: is the seasonally dependent ovary stage a factor to consider when using kidney Macs as biomarkers? To tackle this problem, the relative amount of pigmented Macs in kidney (head, trunk, and tail portions) was stereologically estimated in Ohrid trout, and related with the breeding status. The amount of Macs significantly increased from pre vitellogenesis to late vitellogenesis and showed a decreasing trend then after, with lower values noted after spawning in the head (1.9% versus 7.5% versus 2.0%), trunk (1.8% versus 7.5% versus 2.5%), and tail (2.5% versus 6.7% versus 2.9%) kidney. The decrease seen at spawning was significant in head and trunk kidney, and at post spawning it was significant for all kidney portions. The amounts of Macs were positively correlated with the ovary relative weights and plasma estradiol levels. We proved for the first time that fish kidney pigmented Macs can vary in amount during the breeding cycle. Our data, combined with literature, strongly support that the sex‐steroid profile and kidney status‐seasonal remodeling both influence the Macs pool; likely not only in female trout. So, while increases in Macs may warn of ecosystem problems, we show that using kidney Macs for biomonitoring should also take into account seasonally, particularly that related with ovary maturation. Microsc. Res. Tech. 2011. © 2011 Wiley Periodicals, Inc. 相似文献
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Vera Kmonickova Michaela Frolikova Klaus Steger Katerina Komrskova 《International journal of molecular sciences》2020,21(23)
The LINC (LInker of Nucleoskeleton and Cytoskeleton) complex is localized within the nuclear envelope and consists of SUN (Sad1/UNc84 homology domain-containing) proteins located in the inner nuclear membrane and KASH (Klarsicht/Anc1/Syne1 homology domain-containing) proteins located in the outer nuclear membrane, hence linking nuclear with cytoplasmic structures. While the nucleoplasm-facing side acts as a key player for correct pairing of homolog chromosomes and rapid chromosome movements during meiosis, the cytoplasm-facing side plays a pivotal role for sperm head development and proper acrosome formation during spermiogenesis. A further complex present in spermatozoa is involved in head-to-tail coupling. An intact LINC complex is crucial for the production of fertile sperm, as mutations in genes encoding for complex proteins are known to be associated with male subfertility in both mice and men. The present review provides a comprehensive overview on our current knowledge of LINC complex subtypes present in germ cells and its central role for male reproduction. Future studies on distinct LINC complex components are an absolute requirement to improve the diagnosis of idiopathic male factor infertility and the outcome of assisted reproduction. 相似文献
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