Reconstitution of hepatic tissue architectures from fetal liver cells obtained from a three-dimensional culture with a rotating wall vessel bioreactor

Reconstitution of tissue architecture in vitro is important because it enables researchers to investigate the interactions and mutual relationships between cells and cellular signals involved in the three-dimensional (3D) construction of tissues. To date, in vitro methods for producing tissues with highly ordered structure and high levels of function have met with limited success although a variety of 3D culture systems have been investigated. In this study, we reconstituted functional hepatic tissue including mature hepatocyte and blood vessel-like structures accompanied with bile duct-like structures from E15.5 fetal liver cells, which contained more hepatic stem/progenitor cells comparing with neonatal liver cells. The culture was performed in a simulated microgravity environment produced by a rotating wall vessel (RWV) bioreactor. The hepatocytes in the reconstituted 3D tissue were found to be capable of producing albumin and storing glycogen. Additionally, bile canaliculi between hepatocytes, characteristics of adult hepatocyte in vivo were also formed. Apart from this, bile duct structure secreting mucin was shown to form complicated tubular branches. Furthermore, gene expression analysis by semi-quantitative RT-PCR revealed the elevated levels of mature hepatocyte markers as well as genes with the hepatic function. With RWV culture system, we could produce functionally reconstituted liver tissue and this might be useful in pharmaceutical industry including drug screening and testing and other applications such as an alternative approach to experimental animals.     

Electrochemical DNA methylation detection for enzymatically digested CpG oligonucleotides

We describe the electrochemical detection of DNA methylation through the direct oxidation of both 5-methylcytosine (mC) and cytosine (C) in 5'-CG-3' sequence (CpG) oligonucleotides using a sputtered nanocarbon film electrode after digesting a longer CpG oligonucleotide with endonuclease P1. Direct electrochemistry of the longer CpG oligonucleotides was insufficient for obtaining the oxidation currents of these bases because the CG rich sequence inhibited the direct oxidation of each base in the longer CpG oligonucleotides, owing to the conformational structure and its very low diffusion coefficient. To detect C methylation with better quantitativity and sensitivity in the relatively long CpG oligonucleotides, we successfully used an endonuclease P1 to digest the target CpG oligonucleotide and yield an identical mononucleotide 2'-deoxyribonucleoside 5'-monophosphate (5'-dNMP). Compared with results obtained without P1 treatment, we achieved 4.4 times higher sensitivity and a wider concentration range for mC detection with a resolution capable of detecting a subtle methylated cytosine difference in the CpG oligonucleotides (60mer).     

A case of neutropenic enterocolitis in high dose chemotherapy with peripheral blood stem cell transplantation for relapsed testicular tumor

High dose chemotherapy with peripheral blood stem cell transplantation (PBSCT) is safe and useful for the treatment of refractory or relapsed testicular tumors, but is sometimes accompanied by serious complications. We report a case of relapsed testicular tumor complicated by neutropenic enterocolitis during high dose chemotherapy with PBSCT.     

Observations of seismic electromagnetic phenomena in VHF band by dual frequency method

Seismic electromagnetic (EM) phenomena have been observed at many laboratories with specially designed systems. Recently we can utilize radio receivers, personal computers, and communication systems with higher performances and reasonable cost. It is possible to observe seismic EM phenomena not only by specially designed systems but also by a simple observation system integrated by these devices and systems. We have created an observation system for seismic EM phenomena with them in the very high frequency (VHF) band, from 76 MHz to 90 MHz, assigned for FM broadcasting exclusive use in Japan. We are surrounded by many EM waves, such as broadcasting, communication usage, and man‐made noises. Thus, we have newly developed the dual frequency observation method. In this paper, we describe an observation method that can identify whether a received signal is a broadband EM wave or an artificial FM radio wave. Next, the observation results for broadband EM waves from galactic noise and solar flares are presented. Then, the observation results for FM radio waves reflected from sporadic E layer and the Leonid meteors are shown. Finally, the observation results for the detected broadband EM waves associated with the Geiyo earthquake are described. © 2006 Wiley Periodicals, Inc. Electr Eng Jpn, 155(4): 36–44, 2006; Published online in Wiley InterScience ( www.interscience.wiley.com ). DOI 10.1002/eej.20350     

Vertical seismic response of overhead crane

Vertical seismic response behavior is an important issue for the seismic design of equipments. The equipment, which is comparatively soft and unrestrained vertically, may resonate and its response is significantly magnified under vertical seismic excitation. Overhead crane is an example of equipment that is unrestrained vertically. The dynamic behavior of an 150-ton-capacity overhead crane under vertical seismic excitation was investigated by scale model excitation test and nonlinear time history analysis. The excitation tests were performed with several input levels and the vertical response with each input level was obtained. The simulation analysis approximately corresponded to the results of the excitation test.     

水环境对热塑性复合材料层合板分层疲劳裂纹扩展的影响

本文研究了水环境对ICIAPC-2热塑性预浸料(AS4/PEEK)制成的单向复合材料层合板分层疲劳裂纹扩展的影响.用双悬臂梁试件进行了I型张开位移加载试验.试验环境为23℃水中和50℃水中.水环境未引起很大的不利影响.用电子显微镜和机理分析讨论了水环境对CF/PEEK和CF/环氧两种层合板的差异.     

Advanced Wireless Access System

The key words for the future telecommunications are personalization, multi-media services and high accessibility to the network. These trends naturally lead to radio media application to access links. In this sense, this paper proposes an advanced wireless access system with a capacity supporting broadband video and data services.Concept of the system can be defined as a new radiocommunication category with advantages of both fixed and mobile systems.The system is designed to transport ATM-based signals using SHF (or EHF) bands to wireless terminals, interconnected with optical fibers at the network side. The basic system configuration is presented as well as examples of system parameters.For radio aspects the maximum service coverages of the radio base stations are calculated based on availability consideration. Preferred frequency bands for actual operation are also discussed.