Crystallization and preliminary X-ray analysis of a 1:1 complex between a designed monomeric interferon-gamma and its soluble receptor

A variant of human interferon-gamma (IFN-gamma) has been created in which the two chains of the homodimeric cytokine were linked N- to C-terminus by an eight residue polypeptide linker. The sequence of this linker was derived from a loop in bira bifunctional protein, and was determined from a structural database search. This "single-chain" variant was used to create an IFN-gamma molecule that binds only a single copy of the alpha-chain receptor, rather than the 2 alpha-chain receptor: 1 IFN-gamma binding stoichiometry observed for the native hormone. Crystals have been grown of a 1:1 complex between this single-chain molecule and the extracellular domain of its alpha-chain receptor. These crystals diffract beyond 2.0 A, significantly better than the 2.9 A observed for the native 2:1 complex. Density calculations suggest these crystals contain two complexes in the asymmetric unit; a self-rotation function confirms this conclusion.     

Biosynthesis of progesterone derived neurosteroids by developing avian CNS: in vitro effects on the GABAA receptor complex

Although enthusiasm for measuring health-related quality of life (HRQL) in clinical trials exists, information is limited on the meaning of scores. We examined the relation between scores from the 34-item Medical Outcomes Study HIV Health Survey (MOS-HIV) and the more detailed HIV Overview of Problems-Evaluation System (HOPES) using the responses of 318 HIV-infected outpatients being treated in Los Angeles and Baltimore. With the HOPES problem statements as independent variables, statistically significant predictors of the variation in MOS-HIV scores for the Physical Function, Mental Health, and Energy/Fatigue scales were identified using stepwise regression. Approximately 60% to 70% of the variation in each of the scores was explained by five to seven different HOPES problem statements, with a single item explaining 47% to 59% of the variation. We created illustrative profiles for each of the three MOS-HIV scales using the HOPES items identified in the regressions. Independent of the scale, persons scoring in the top MOS-HIV quartile tended to report few if any problems, whereas a decline in score to the next quartile was characterized by functional difficulties (e.g., "HIV interferes with work"). The onset of specific problems might trigger further evaluation and potential intervention from health care providers to help maintain patient functioning.     

A closed-loop healthcare processing approach based on deep reinforcement learning

Multimedia Tools and Applications - In healthcare, the human body is a controlled input-output system, which generates different observations with the variations of external interventions. The...     

DLSA: Delay and Link Stability Aware Routing Protocol for Flying Ad-hoc Networks (FANETs)

Flying Ad-hoc Network (FANET) is a new class of Mobile Ad-hoc Network in which the nodes move in three-dimensional (3-D) ways in the air simultaneously. These nodes are known as Unmanned Aerial Vehicles (UAVs) that are operated live remotely or by the pre-defined mechanism which involves no human personnel. Due to the high mobility of nodes and dynamic topology, link stability is a research challenge in FANET. From this viewpoint, recent research has focused on link stability with the highest threshold value by maximizing Packet Delivery Ratio and minimizing End-to-End Delay. In this paper, a hybrid scheme named Delay and Link Stability Aware (DLSA) routing scheme has been proposed with the contrast of Distributed Priority Tree-based Routing and Link Stability Estimation-based Routing FANET’s existing routing schemes. Unlike existing schemes, the proposed scheme possesses the features of collaborative data forwarding and link stability. The simulation results have shown the improved performance of the proposed DLSA routing protocol in contrast to the selected existing ones DPTR and LEPR in terms of E2ED, PDR, Network Lifetime, and Transmission Loss. The Average E2ED in milliseconds of DLSA was measured 0.457 while DPTR was 1.492 and LEPR was 1.006. Similarly, the Average PDR in %age of DLSA measured 3.106 while DPTR was 2.303 and LEPR was 0.682. The average Network Lifetime of DLSA measured 62.141 while DPTR was 23.026 and LEPR was 27.298. At finally, the Average Transmission Loss in dBm of DLSA measured 0.975 while DPTR was 1.053 and LEPR was 1.227.

     

Classification of multi-lingual tweets,into multi-class model using Naïve Bayes and semi-supervised learning

Multimedia Tools and Applications - Twitter is a social media platform which has been proven to be a great tool for insights of emotions about products, policies etc. through a 280-character...     

Egocentric visual scene description based on human-object interaction and deep spatial relations among objects

Multimedia Tools and Applications - Visual Scene interpretation is one of the major areas of research in the recent past. Recognition of human object interaction is a fundamental step...     

New features of 23S ribosomal RNA folding: the long helix 41-42 makes a "U-turn" inside the ribosome

23S rRNA from Escherichia coli was cleaved at single internucleotide bonds using ribonuclease H in the presence of appropriate chimeric oligonucleotides; the individual cleavage sites were between residues 384 and 385, 867 and 868, 1045 and 1046, and 2510 and 2511, with an additional fortuitous cleavage at positions 1117 and 1118. In each case, the 3' terminus of the 5' fragment was ligated to radioactively labeled 4-thiouridine 5'-,3'-biphosphate ("psUp"), and the cleaved 23S rRNA carrying this label was reconstituted into 50S subunits. The 50S subunits were able to associate normally with 30S subunits to form 70S ribosomes. Intra-RNA crosslinks from the 4-thiouridine residues were induced by irradiation at 350 nm, and the crosslink sites within the 23S rRNA were analyzed. The rRNA molecules carrying psUp at positions 867 and 1117 showed crosslinks to nearby positions on the opposite strand of the same double helix where the cleavage was located, and no crosslinking was detected from position 2510. In contrast, the rRNA carrying psUp at position 384 showed crosslinking to nt 420 (and sometimes also to 416 and 425) in the neighboring helix in 23S rRNA, and the rRNA with psUp at position 1045 gave a crosslink to residue 993. The latter crosslink demonstrates that the long helix 41-42 of the 23S rRNA (which carries the region associated with GTPase activity) must double back on itself, forming a "U-turn" in the ribosome. This result is discussed in terms of the topography of the GTPase region in the 50S subunit, and its relation to the locations of the 5S rRNA and the peptidyl transferase center.     

Perceptions of caring. Comparison of antepartum and postpartum patients

An exploratory, comparative survey design was used to describe and compare similarities and differences in perception of caring behaviors between hospitalized antepartum and short-stay postpartum patients. Forty-two patients completed the 63-item Caring Behavior Assessment instrument (CBA). The scale, based on Watson's theory of caring, contains seven categories. There were no statistically significant differences in age, education, gravida, or para between the two groups. Significantly more women in the postpartum group were married. There were no statistically significant differences or correlations in the total CBA scores and the demographic variables. No statistically significant differences in total CBA scores were found between antepartum participants and postpartum participants. These findings corroborate the results of "caring" studies in other patient populations, that satisfying basic needs--that is, evidence of technical knowledge and skills--is more important to patients than meeting higher order needs.     

The anterior and posterior selective temporal lobe amobarbital tests: angiographic, clinical, electroencephalographic, PET, SPECT findings, and memory performance

The techniques, results, and problems of three types of selective temporal lobe (TL) amobarbital procedures (balloon technique with temporary occlusion of the internal carotid artery distal to the origin of the anterior choroidal artery (acha) [n = 19]; selective anterior catheterization of the acha [n = 20]; and selective catheterization of the peduncular P2-segment of the posterior cerebral artery [n = 5]) are described in a group of 40 patients with medically refractory complex partial seizures of mesial TL origin. Selective amobarbital tests were carried out before surgery to predict the memory deficit after an intended selective amygdalohippocampectomy. The effects of selective anaesthetization of TL were correlated with clinical data, pattern and duration of amobarbital induced EEG changes, and performance on verbal and nonverbal memory tasks measured during the test. In 4 patients the effect of selective amobarbital injection on regional and global metabolism was studied with 18F-FDG-PET, with the PET tracer being injected intravenously immediately after amobarbital. More recently in 2 patients the vascular territory perfused by amobarbital in the acha test was studied with SPECT using 99m Tc ECD injected immediately prior to the amobarbital into the acha. Whereas the PET studies showed a rather widespread and bilateral amobarbital-induced decrease of metabolism, the SPECT studies confirmed the selective distribution of the tracer in the vascular territory of the acha, i.e., in amygdala and hippocampus. The comparison of selective TL amobarbital test performance with postoperative neuropsychological performance showed that the predictive value of this test is rather good for the postoperative verbal memory but underestimates postoperative nonverbal ("figural") memory performance.     

Individual differences in the vibrotactile perception of a "simple" pattern set

Discriminative capacities for vibrotactile spatiotemporal patterns were examined in 62 college students with three tasks: identification, masking, and discrimination of the letters "X" and "O" presented tactually on the Optacon, a reading machine for blind persons. Individual differences in performance and interrelations among scores within and across paradigms were explored. In identification, most persons quickly achieved consistently better than 90% performance, but others failed to identify the patterns above 80%, even after prolonged training. The same performance variance was found when the task was repeated by 23 Naval student pilots. Masking and discrimination measured susceptibility to interference when patterns followed one another closely in time. The resulting functions were typical, with poorest performance at short stimulus onset asynchronies. Again, a wide range of performance was seen. Individual performance, however, appeared to be consistent across tasks, suggesting that abilities in a variety of pattern-perception tasks might be predictable.