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41.
Gene amplification in a p53-deficient cell line requires cell cycle progression under conditions that generate DNA breakage 总被引:1,自引:0,他引:1
Amplification of genes involved in signal transduction and cell cycle control occurs in a significant fraction of human cancers. Loss of p53 function has been proposed to enable cells with gene amplification to arise spontaneously during growth in vitro. However, this conclusion derives from studies employing the UMP synthesis inhibitor N-phosphonacetyl-L-aspartate (PALA), which, in addition to selecting for cells containing extra copies of the CAD locus, enables p53-deficient cells to enter S phase and acquire the DNA breaks that initiate the amplification process. Thus, it has not been possible to determine if gene amplification occurs spontaneously or results from the inductive effects of the selective agent. The studies reported here assess whether p53 deficiency leads to spontaneous genetic instability by comparing cell cycle responses and amplification frequencies of the human fibrosarcoma cell line HT1080 when treated with PALA or with methotrexate, an antifolate that, under the conditions used, should not generate DNA breaks. p53-deficient HT1080 cells generated PALA-resistant variants containing amplified CAD genes at a frequency of >10(-5). By contrast, methotrexate selection did not result in resistant cells at a detectable frequency (<10(-9)). However, growth of HT1080 cells under conditions that induced DNA breakage prior to selection generated methotrexate-resistant clones containing amplified dihydrofolate reductase sequences at a high frequency. These data demonstrate that, under standard growth conditions, p53 loss is not sufficient to enable cells to produce the DNA breaks that initiate amplification. We propose that p53-deficient cells must proceed through S phase under conditions that induce DNA breakage for genetic instability to occur. 相似文献
42.
2003年夏天,广州大学城广州外语外贸大学一期项目的单体设计在相当短的设计周期中紧张地铺开。 相似文献
43.
44.
官能团化MCM-41在分离低碳醇-水混合物中的应用 总被引:2,自引:2,他引:0
采用一步法水热合成了介孔材料氯丙基官能团化的MCM-41(Cl(CH2)3-MCM-41)、氨丙基官能团化MCM-41(NH2(CH2)3-MCM-41)、甲基官能团化MCM-41(CH3-MCM-41)和氯丙基官能团化硅胶,并考察了它们作为气相色谱固定相分离低碳醇-水混合物的情况。实验结果表明,Cl(CH2)3-MCM-41能够分离低碳醇-水混合物中的各组分,随水、甲醇、乙醇、异丙醇、正丁醇极性的减小,各分子与Cl(CH2)3-MCM-41的作用力增强,保留时间延长。氯丙基官能团化硅胶、NH2(CH2)3-MCM-41)和CH3-MCM-41均不能分离低碳醇-水混合物,表明介孔材料规则的孔道结构、表面嫁接基团等对低碳醇-水混合物的分离有重要影响。同时考察了Cl(CH2)3-MCM-41的氯丙基负载量对低碳醇-水混合物分离效果的影响,结果表明,当氯丙基的摩尔分数为15%时,分离效果最佳。 相似文献
45.
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47.
大变量逻辑函数最佳覆盖问题研究 总被引:2,自引:0,他引:2
逻辑函数的最佳覆盖,一直是逻辑综合领域的关键环节。尤其是大变量逻辑函数最佳覆盖,对复杂的逻辑综合更为重要,但也更加困难。本文在对逻辑覆盖算法研究的基础上,提出了适合大变量逻辑函数最佳覆盖的Beister改进算法。经过大量算题的测试表明,改进的列覆盖算法在时间复杂度和选择效果方面均优于Beister算法。 相似文献
48.
A bibliometric study of China’s semiconductor literature compared with other major asian countries 总被引:1,自引:0,他引:1
In this paper we compare the scientific research in the semiconductor-related field in China with some other major nations
in Asia. It is based on the bibliometric information from SCI-Expanded database during the time period of 1995–2004. We show
that China has been developing fast in semiconductor research, and become the second productive country in Asia as reflected
by the publication profile. The evidences indicate a significant increasing trend in the research efforts and readership among
Asian countries. Similar to the scientists in Japan and South Korea, Chinese scientists were more inclined to work in larger
groups, typically 4 or more authors. The assessment of research quality is further conducted based on citation-based measures.
As benchmarks, two western countries, namely USA and Germany, have been compared in the citation analysis. It is revealed
that the impacts of research outputs in the Asian countries, except for Japan, have been badly incommensurate with their devoted
research efforts compared with USA and Germany. Like most of other Asian countries the research results of Chinese scientists
in semiconductor have a low international visibility despite their strong research efforts and increasingly large domestic
readership. The application of Leimkuhler curve illustrates vividly the inequality of citation times among the compared countries.
Furthermore, the Gini Indices of each country and each pair of countries are calculated which illustrates again the inequality
of informetric productivities. 相似文献
49.
50.
湿法生产线的技术更新改造有异地新建新型干法生产线和原地改成湿磨干法生产线二种。浙江虎山集团(?)3.0 m×88.68 m湿法线的改造,经方案比较和生产原料的状况,采用了湿磨干烧方案。其中,料浆脱水采用了真空吸滤法,浇成系统采用了南京院专有技术。改造投产后,获得了优质、高产、低耗和安全生产的技改效果。 相似文献