PURIFICATION AND PROPERTIES OF TRANSGLUTAMINASE FROM STREPTOVERTICILLIUM MOBARAENSE

Transglutaminase (TGase) was separated from the culture broth of an isolated strain of Streptoverticillium mobaraense. The crude enzyme was prepared by centrifugation, ultrafiltration, precipitation by alcohol, centrifugation and freeze‐drying. The yield after these processes was 65–70%. Then the enzyme was purified to homogeneity by chromatography on CM‐cellulose and Sephadex G‐75 on which the yields were about 70% and 80%, respectively; the purified folds reached 2.5–4.7 and 1.08–2.06, respectively. The molecular weight of this TGase was 39,500–40,100 Da by gel filtration chromatography. Optimum enzyme activity was observed in the pH range of 5.0–7.0, and it was maintained stable at 20–40C. The optimal temperature and pH was 52C and 6.0, respectively. At 1 mM and 5 mM metal ion or inhibitors concentration, TGase activity was strongly inhibited by Zn²⁺ and NEM, and not affected obviously by Ba²⁺, Ca²⁺, Co²⁺, Cu²⁺, Fe²⁺, Fe³⁺, Mg²⁺, Mn²⁺, Na⁺ as well as PMSF and EDTA. The effects of these additions on this TGase were compared with those of other microbial TGases.     

公司大门工程建设施工

大门小品建筑属多项目综合性工程。本文作者从管理者的角度 ,通过对公司大门及附属工程施工质量控制的总结 ,说明了质量控制应贯穿于工程施工的全过程     

Photoluminescence and X-ray Diffraction of Distributed Bragg Reflector

Spectral and structural characteristics of distributed Bragg reflector (DBR) in vertical-cavity surface-emitting lasers were studied with photoluminescence and double- crystal X- ray diffraction measurement. The expected high quality epitaxial DBR structure was verified. In the X- ray double- crystal rocking curves of DBR the zeroth- order peak, the first and second order satellite peaks were measured. Splitting of diffraction peak appeared in the rocking curves was analyzed. The effects of introduced deep energy levels on the structural perfection and optical properties were discussed.     

Experimental Research on Erbium-doped Superfluorescent Fiber Source

The effects of the variations in fiber length, fiber mirror reflectance on efficiency and output power are experimentally investigated for erbium-doped double pass backward superfluorescent fiber sources (SFSs). The influence of fiber length on mean wavelength stability (MWS) has also been demonstrated. By incorporating a short section of un-pumped erbium-doped fiber (EDF) at the output port, the pump power dependent on MWS becomes independent of pumped EDF length. This is a novel phenomenon that hasn‘t been reported up to now,and should be helpful to SFS fabrication and theory analysis. By using a fiber Michelson interferometer as spectrum slicing component,a multi-wavelength fiber source (MWFS) with ～20 channels (from 1542nm to 1559nm) is got. The MWFS has a channel spacing of ～0. 8 nm which satisfies ITUstandard. The intensity fluctuation among channels is less than 0.5dB,and the extinction ratio of all channels is above 14dB. This kind of MWFS should be useful to wavelength division multiplexing systems.     

一种动态开关电容运算放大器共模负反馈电路

本文主要介绍了一种应用于Cascode结构运放中的开关电容共模负反馈电路，它具有稳定性好、对运放频率特性影响小、不消耗额外功率等优点。对电路运用Hspice进行了模拟，并给出了模拟结果。     

项目管理软件的开发思路

详细介绍了项目管理的发展及项目管理软件的现状。通过对P3管理软件的分析论述，得出项目管理软件系统的开发方案。     

固定背景下的随机波形提取模型

分析了当前对于从固定背景下的特征符号与背景符号相粘连的图表中提取信息所存在的问题，设计了适用于此类问题的方法模型。提出了一种在固定背景下通过背景符号检测和背景符号擦除来实现随机波形提取的方法。     

无后坐控爆拆除砖砌水塔的实践

介绍了对一砖砌水塔进行无后坐拆除爆破时爆破参数的选取,以及控制后坐的措施.实践证明:用控制爆破方法,选择合理的爆破参数完全可以控制水塔在倾倒时的后坐.     

单轴-平面W型复合锶铁氧体的制备及其吸波特性研究

用草酸盐共沉淀和微量掺杂法研制六角晶系W型Ni,Zn,Co,Al多元复合锶铁氧体，通过调节Ni2W同Co2W的摩尔比例，可使该种铁氧体易磁化方向从C单轴型向垂于C轴的平面型转化，控制置换锶铁氧体中部分Fe3 的微量掺杂Al3 ，可以调整4πMs值，提高磁晶各向异性场，测试分析了5种不同含Co量的W型复合锶铁氧体粉料涂层的吸波特性，发现其自然共振吸收峰随Co含量增加向低频方向偏移，最高吸收峰可达32．8dB，是适用于电子隐形技术的高品位的微波吸收剂。     

潜油螺杆泵采油系统设计与应用技术分析

潜油螺杆泵采油技术在某些方面具有其他人工举升技术不可比拟的优点，特别适合于我国渤海湾一带粘油区、海上平台的采油作业，但这项技术在我国实际开发应用的时间还比较短，人们对这项技术的了解还不够深入，很多单项技术还有待于更多的人去关注和研究.介绍了潜油螺杆泵采油系统的结构，总结了该项采油技术与其他常用采油技术相比较的特点，系统地阐述了国内外的发展状况，分析了以往投入生产试运行时系统失效的原因并提出了相应的解决办法，最后探讨了系统设计技术的发展趋势.