Online analysis of in vitro resistance to antimalarial drugs through nonlinear regression

Malaria remains one of the leading causes of morbidity and mortality worldwide which is partly due to the emergence of the parasite resistance to antimalarial drugs. In vitro testing of drugs allows an early detection of resistance. The common parameter used for the monitoring of resistance is the concentration that inhibits 50% of the parasite's activity (IC₅₀). Various methods of calculation are already used but none of them consider new available tools nor display the precision of IC₅₀ estimation. We proposed an approach based on the inhibitory sigmoid E_max model, which is often used in pharmacology, with estimation of IC₅₀ through nonlinear regression using a standard function of the R software. To facilitate the usage of that tool we have developed an online version available on the website ICEstimator (ICEstimator website http://www.antimalarial-icestimator.net/, 2009) [1]. This website is used by various teams in the world and the user can do the analysis without knowing R using the GUI. This article describes version 2.1 of this website and shows illustration on five different real examples.     

Experimental validation of the linear drive train for a total artificial heart system

In industrialized countries cardiovascular diseases are the major cause of death. Beside heart transplants, which are a limited option due to the limited number of available human donor hearts, Total Artificial Hearts (TAHs) are the only therapy available for some patients with terminal heart diseases. For various reasons a total implantable artificial heart is desirable, but also sets restrictions in terms of weight and dimensions due to the limited space in the human thorax. Therefore a precise requirement profile is needed for the drive design to provide sufficient force for the blood pump and to avoid oversizing of the drive and to prevent blood damage by overheating.     

学分制学籍管理存在的问题及对策

介绍了重庆科技学院实行学分制的做法和在学籍管理方面采取的相应措施,分析了学分制学籍管理中存在的问题,提出了改善学分制学籍管理工作的建议.     

Severe gastrointestinal hemorrhage due to Mycobacterium avium complex in a patient receiving immunosuppressive therapy

Intense immunosuppressive therapy is used frequently for treatment of systemic vasculitides, collagenoses, rapidly progressive glomerulonephritis, and after organ transplantation. Numerous serious treatment-related side effects include localized or disseminated opportunistic infections, and require careful monitoring of immunosuppressed patients. Gastrointestinal infections with Mycobacterium avium complex (MAC) or other nontuberculous mycobacteria have been previously identified in HIV seropositive patients only. We now report the first case of an HIV seronegative patient who received immunosuppressive therapy for rapidly progressive glomerulonephritis. The patient presented with severe lower gastrointestinal bleeding and was diagnosed to have ulcerative colitis due to infection with MAC. The patient recovered promptly after administration of antimycobacterial therapy. MAC infection should be included in the differential diagnosis of gastrointestinal bleeding in all immunodeficient patients. The significance of repeated colonoscopy to obtain multiple biopsy specimens with histological examination for foam cells and specific staining for acid-fast organisms is emphasized.     

The emergence of coherence over the course of decision making.

Previous research has indicated that decision making is accompanied by an increase in the coherence of assessments of the factors related to the decision alternatives. In the present study, the authors investigated whether this coherence shift is obtained before people commit to a decision, and whether it is obtained in the course of a number of other processing tasks. College students were presented with a complex legal case involving multiple conflicting arguments. Participants rated agreement with the individual arguments in isolation before seeing the case and after processing it under various initial sets, including playing the role of a judge assigned to decide the case. Coherence shifts were observed when participants were instructed to delay making the decision (Experiment 1), to memorize the case (Experiment 2), and to comprehend the case (Experiment 3). The findings support the hypothesis that a coherence-generating mechanism operates in a variety of processing tasks, including decision making. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

An Integrated DSS for Groundwater Management Based on Remote Sensing. The Case of a Semi-arid Aquifer in Morocco

A Decision Support System has been set up as the result of a fruitful cooperation between several public and research institutions in the framework of a large cooperation program. The DSS aims to compare spatially and temporally sectorial water demands of the Haouz-Mejjate plain (Morocco) in regard to available surface and groundwater resources. It is composed of a tool for satellite estimation of Agricultural Water Demand (SAMIR), a tool for integrated water resources planning (WEAP) and a groundwater model (MODFLOW) each of them relying on a common Geographical Information System not described here. The DSS is operating on a monthly time scale. Agricultural water demand accounts for about 80?% of the total demand. In areas where groundwater abstraction is difficult to quantify by direct methods, multitemporal remote sensing associated to the FAO methodology is a simple and efficient alternative to estimate Evapotranspiration (ET). In this work, a monthly estimate of ET from irrigated areas is derived from freely available MODIS NDVI for the 2001?C2009 period. An important part of the paper deals with the validation of these estimates with eddy covariance flux measurements installed on different irrigated crops of the region. Results are satisfactory with a minus 6.5?% error per year on the monthly time scale. This preprocessing allows to dichotomize irrigated versus non-irrigated areas, and then, to estimate groundwater abstraction in subareas distinguishing by their operating modes: traditional, dam or privately irrigated. A dynamic linkage between MODFLOW and WEAP transfers the results of one model as input data to the other. The model restitutes both spatial and temporal variations in head charges and allows the calculation of the ground water balance. After calibration, piezometric validation is acceptable for the majority of the 21 head control points.     

Xanthylium salts formation involved in wine colour changes

Summary The reaction of (+)-catechin in wine-like model solution was investigated. First appearance of colourless dimeric compounds consisting of two flavanol units linked by carboxy-methine bridge was observed. Their isolation and further incubation was found to yield two types of yellowish pigments showing visible absorption maxima at 440 and 460 nm, respectively. Mass spectroscopy (MS) spectral analysis showed that the first type were xanthylium salt pigments formed by dehydration of the colourless compounds followed by an oxidation process. The loss of a water molecule was shown to take place between two A ring hydroxyl groups of the colourless dimers. The second type were shown to be ester derivatives of the first ones. Thus ethylester of xanthylium salt was obtained and fully characterized by mass and nuclear magnetic resonance (NMR) spectroscopy. Esterification was found to involve the colourless compound before dehydration and thus a general scheme for xanthylium salt formation was postulated. The proposed scheme constitutes a new xanthylium formation pathway as up to now only anthocyanin-flavanol reactions were supposed to form xanthylium salt derivatives during wine ageing. This work also provides new support to the contribution of xanthylium salt in colour evolution observed during wine ageing which is generally expressed in an increase of absorption in the 400–500 nm, region of xanthylium salt absorption maxima.     

Degradation Products of Tryptophan in Cell Culture Media: Contribution to Color and Toxicity

Biomanufacturing processes may be optimized by storing cell culture media at room temperature, but this is currently limited by their instability and change in color upon long-term storage. This study demonstrates that one of the critical contributing factors toward media browning is tryptophan. LC-MS technology was utilized to identify tryptophan degradation products, which are likely formed primarily from oxidation reactions. Several of the identified compounds were shown to contribute significantly to color in solutions but also to exhibit toxicity against CHO cells. A cell-culture-compatible antioxidant, a-ketoglutaric acid, was found to be an efficient cell culture media additive for stabilizing components against degradation, inhibiting the browning of media formulations, and decreasing ammonia production, thus providing a viable method for developing room-temperature stable cell culture media.