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21.
22.
Brefeldin A (BFA) has been reported to cause disassembly of the Golgi. We have used three-dimensional (3-D) high-resolution scanning electron microscopy (HRSEM) to investigate these effects in human skin fibroblast cells. The spontaneous reassembly during prolonged exposure to BFA and some effects of forskolin were observed. A BFA concentration of 5μg/ml caused Golgi complexes to become vesicular, resulting in a progressive decrease in the size of the Golgi. Morphologic changes were visible within 2 min of BFA incubation, and by 30 min no identifiable Golgi could be found. Spontaneous reassembly of the Golgi apparatus upon the removal of the BFA or with continued long-term exposure with BFA could not be confirmed. Preliminary experiments with forskolin were not effective in reversing or inhibiting the effects of BFA in human fibroblast cells grown in culture. This inability for spontaneous reassembly and nonreversal by forskolin may reflect a differential effect of BFA in various cell types. HRSEM has proven to be useful for observing 3-D morphologic effects of BFA in Golgi. 相似文献
23.
Ga -Er Yu J. Parrick M. Edirisinghe D. Finch B. Ralph 《Journal of Materials Science》1994,29(6):1680-1685
Proton nuclear magnetic resonance (1H NMR) spectroscopy, Fourier transform infrared (FT-IR) spectroscopy and gas chromatography (GC) have been used to study the products of ammonolysis of dichloromethylsilane in diethyl ether at 0 °C. Results indicate that the major products are trimethylcyclotrisilazane and tetramethylcyclotetrasilazane. Hydrolysis of the cyclosilazanes has also been qualitatively investigated. A simple and useful method for the identification of cyclosilazanes has been developed using the 1H NMR technique. 相似文献
24.
Summary the simulation described in Part I was applied to random step polyaddition of a trifunctional monomer and the results were compared with exact solution for an infinite system. The gel point conversions, the weight-average degree of polymerization before (P
w) and beyond (P
w,sol) the gel point, the sol fraction and the cycle rank were used for comparison. The best way for detection of the gel point conversion is the extrapolation of the gel fraction, w
g, to w
g=0. The largest fluctuations are exhibited by P
w and P
w,sol. To get results closer to the exact ones, one can repeat several experiments with smaller number of units or increase the number of units, the former way being somewhat more economical. Typical orders of magnitude used were 107 monomeric units. 相似文献
25.
The inhibiting action of N-arylpyrroles on aluminium in 0.17 mol dm–3 hydrochloric acid solution, in the temperature range 20–60 °C, was studied using potentiodynamic and electrochemical impedence spectroscopy techniques. The inhibiting efficiency of both investigated compounds 1-(2-fluorophenyl)-2,5-dimethylpyrrole (compound A) and 1-(2-fluorophenyl)-2,5-dimethylpyrrole-3-carbaldehyde (compound B) slightly increases with increasing temperature of the corrosive solution. Inhibitor adsorption on the elecrode surface decreases the apparent activation energy of the hydrogen evolution reaction. Compound A follows the Temkin adsorption isotherm, while its carbaldehyde derivative follows the Langmuir isotherm. The results of the apparent energy of activation and the standard free energy of adsorption point to stronger chemisorption of the compound A. However, due to additional condensation of molecules with carbaldehyde groups on the electrode surface, the carbaldehyde derivative exhibits even better inhibiting efficiency than compound A. The kinetic corrosion parameters, analysed in terms of the impedance data, show a satisfactory agreement with those obtained by the potentiodynamic method. 相似文献
26.
JA Tumlin JP Lea CE Swanson CL Smith SS Edge JS Someren 《Canadian Metallurgical Quarterly》1997,99(6):1217-1223
1. PD 81,723 has been shown to enhance binding of adenosine to A1 receptors by stabilizing G protein-receptor coupling ('allosteric enhancement'). Evidence has been provided that in the perfused hearts and isolated atria PD 81,723 causes a sensitization to adenosine via this mechanism. 2. We have studied the effect of PD 81,723 in guinea-pig isolated atrial myocytes by use of whole-cell measurement of the muscarinic K+ current (I[K(ACh)]) activated by different Gi-coupled receptors (A1, M2, sphingolipid). PD 81,273 caused inhibition of I[K(ACh)] (IC50 approximately 5 microM) activated by either of the three receptors. Receptor-independent I[K(ACh)] in cells loaded with GTP-gamma-S and background I[K(ACh)], which contributes to the resting conductance of atrial myocytes, were equally sensitive to PD 81,723. At no combination of concentrations of adenosine and PD 81,723 could an enhancing effect be detected. 3. The compound was active from the outside only. Loading of the cells with PD 81,723 (50 microM) via the patch pipette did not affect either I[K(ACh)] or its sensitivity to adenosine. We suggest that PD 81,723 acts as an inhibitor of inward rectifying K+ channels; this is supported by the finding that ventricular I(K1), which shares a large degree of homology with the proteins (GIRK1/GIRK4) forming I[K(ACh)] but is not G protein-gated, was also blocked by this compound. 4. It is concluded that the functional effects of PD 81,723 described in the literature are not mediated by the A1 adenosine receptor-Gi-I[K(ACh)] pathway. 相似文献
27.
P. Lobotka I. Vávra Š. Gaži A. Plecenik J. Dérer 《Journal of Low Temperature Physics》1997,106(3-4):381-386
A Josephson junction Nb/Si/Nb with a 10 nm thick amorphous silicon barrier is studied. The upper electrode contains a 2 nm
thick sublayer of amorphous phase adjacent to the barrier, as revealed by cross-sectional TEM. Thus, the junction can be considered
as a S-I-N-S system with the N layer represented by amorphous niobium. Peculiarities in the I–V and Δ(T) dependencies are
observed and explained as a consequence of a proximity effect present in the upper electrode. 相似文献
28.
Dr. P. Bošnjakovié Dipl.-Ing. B. Djokié 《Electrical Engineering (Archiv fur Elektrotechnik)》1992,75(2):131-135
Contents This paper analyzes a problem of phase shifting and deseribes a novel method for electronic reactive energy measurement. The proposed measurement structure, composed of an integrator as a phase shifting circuit and a frequency controlled analog-to-pulse-rate converter, provides for the measurement completely in accordance with IEC definition of reactive energy, which is performed with simple but high performance metering device.
Die Blindverbrauchsmessung anhand eines netzsynchronisierten Analog-Frequenz-Wandlers
Übersicht In diesem Artikel wird das Problem der Phasenverschiebung analysiert und eine neue Meßmethode für elektronische Blindverbrauschsmessung aufgezeigt. Die vorgeschlagene Strucktur, bestehend aus einem Integrator als Phasenschieber und netzkontrolliertem Analog-Frequenz-Wandler, ermöglicht die Verwirklichung eines einfachen aber präzisen Elektrizitätszählers zur Messung der Blindenergie, dessen Arbeitsprinzip mit den IEC Empfehlungen vollkommen übereinstimmt.相似文献
29.
N Chevassus-Au-Louis P Congar A Represa Y Ben-Ari JL Ga?arsa 《Canadian Metallurgical Quarterly》1998,95(17):10263-10268
Neuronal migration disorders have been involved in various pathologies, including epilepsy, but the properties of the neural networks underlying disorders have not been determined. In the present study, patch clamp recordings were made from intrahippocampal heterotopic as well as from neocortical and hippocampal neurons from brain slices of rats with prenatally methylazoxymethanol-induced cortical malformation. We report that heterotopic neurons have morphometrical parameters and cellular properties of neocortical supragranular neurons and are integrated in both neocortical and hippocampal networks. Thus, stimulation of the white matter induces both antidromic and orthodromic response in heterotopic and neocortical neurons. Stimulation of hippocampal afferents evokes a monosynaptic response in the majority of heterotopic neurons and a polysynaptic all-or-none epileptiform burst in the presence of bicuculline to block gamma-aminobutyric acid type A inhibition. Furthermore, hippocampal paroxysmal activity generated by bath application of bicuculline can spread directly to the neocortex via the heterotopia in methylazoxymethanol-treated but not in naive rats. We conclude that heterotopias form a functional bridge between the limbic system and the neocortex, providing a substrate for pathological conditions. 相似文献
30.