Quantification of structural changes in acute inflammation by fractal dimension,angular second moment and correlation

The aim of the study was to examine alteration and possible application of fractal dimension, angular second moment, and correlation for quantification of structural changes in acutely inflamed tissue. Acute inflammation was induced by injection of turpentine oil into the right and left hind limb muscles of mice, whereas control animals received intramuscular saline injection. After 12 h, animals were anesthetised and treated muscles collected. The tissue was stained by hematoxylin and eosin, digital micrographs produced, enabling determination of fractal dimension of the cells, angular second moment and correlation of studied tissue. Histopathological analysis showed presence of inflammatory infiltrate and tissue damage in inflammatory group, whereas tissue structure in control group was preserved, devoid of inflammatory infiltrate. Fractal dimension of the cells, angular second moment and correlation of treated tissue in inflammatory group decreased in comparison to the control group. In this study, we were first to observe and report that fractal dimension of the cells, angular second moment, and correlation were reduced in acutely inflamed tissue, indicating loss of overall complexity of the cells in the tissue, the tissue uniformity and structure regularity. Fractal dimension, angular second moment and correlation could be useful methods for quantification of structural changes in acute inflammation.     

Asphaltene precipitation and deposition in oil reservoirs – Technical aspects,experimental and hybrid neural network predictive tools

Precipitation of asphaltene is considered as an undesired process during oil production via natural depletion and gas injection as it blocks the pore space and reduces the oil flow rate. In addition, it lessens the efficiency of the gas injection into oil reservoirs. This paper presents static and dynamic experiments conducted to investigate the effects of temperature, pressure, pressure drop, dilution ratio, and mixture compositions on asphaltene precipitation and deposition. Important technical aspects of asphaltene precipitation such as equation of state, analysis tools, and predictive methods are also discussed. Different methodologies to analyze asphaltene precipitation are reviewed, as well. Artificial neural networks (ANNs) joined with imperialist competitive algorithm (ICA) and particle swarm optimization (PSO) are employed to approximate asphaltene precipitation and deposition with and without CO₂ injection. The connectionist model is built based on experimental data covering wide ranges of process and thermodynamic conditions. A good match was obtained between the real data and the model predictions. Temperature and pressure drop have the highest influence on asphaltene deposition during dynamic tests. ICA-ANN attains more reliable outputs compared with PSO-ANN, the conventional ANN, and scaling models. In addition, high pressure microscopy (HPM) technique leads to more accurate results compared with quantitative methods when studying asphaltene precipitation.     

GEOSTATIONARY STATION-KEEPING BY ION THRUSTERS: GENETIC ALGORITHMS OPTIMIZATION

The orbital control of geostationary satellites requires the periodic execution of manoeuvres for gravitational and solar radiation perturbation compensation. To maintain a fine control of the satellite position, it is usually sufficient to perform orbit corrections every two weeks, firing chemical thrusters. The operational load for the manoeuvre's planning and execution is usually quite low. Innovative technologies for station-keeping are available now, based on high efficiency ion thrusters, which allow a significant propellant saving. They are included in Artemis, a telecommunications satellite developed by the European Space Agency. When low acceleration ion thrusters are adopted, a near continuous orbit control is required; the resulting long firing arcs can be in conflict with some operational or technical constraints. The station-keeping planning is a critical process, since it is not based on periodic impulsive manoeuvres, but rather on quasi-continuous firings. Furthermore the orbit control should be planned more on a seasonal or a yearly cycle rather than on short weekly cycles. Since each manoeuvre is defined by its start time and duration, the total number of parameters involved in a station-keeping optimization process amounts to several tens. The presence of many variables and logical constraints makes the use of classical optimization methods quite complex. The model proposed here is based on genetic algorithms, which allows the convergence to an optimum solution through successive iterations, in which a random set of solutions is progressively selected on the basis of a factor of merit tailored to the target. This paper presents some innovative concepts for station-keeping optimization when ion propulsion is adopted for orbital control; furthermore, the general properties of genetic algorithms are discussed, together with the results obtained in the specific station-keeping application.     

Analysis of hexamidine, dibromohexamidine, dibromopropamidine and chlorhexidine in cosmetic products by high-performance liquid chromatography

A method of determining qualitatively and quantitatively hexamidine, dibromohexamidine, dibromopropamidine and chlorhexidine in cosmetic products by means of paired ion, reverse-phase high-performance liquid chromatography (HPLC) and ultraviolet detection (UV) at 264 nm.
Détermination de l'hexamidine, du dibromohexamidine, du dibromopropamidine et de la chlorhexidine dans les produits cosmétiques     

大青叶抗菌物质提取及抗菌效果研究

研究了用水浸提法抽提大青叶中抗菌物质的方法，并通过抑菌实验检验了对细菌和霉菌的抑菌能力。正交实验结果表明最佳抽提工艺为：料水比1：20，加热时间40min，回流温度100℃：滤纸片法抑菌实验发现：大青叶提抽浓缩液(浓缩比为5：1)对大肠杆菌及枯草杆菌的最低抑菌浓度为2．77％(ml，100ml)左右，青霉，黄曲霉为0．55％(ml／100ml)左右，金黄色葡萄球为0．055％(ml／100ml)左右。     

Removal of bisphenol A and 17α-ethinyl estradiol from landfill leachate using single-walled carbon nanotubes

In this study, the adsorption of bisphenol A (BPA) and 17α-ethinyl estradiol (EE2) from landfill leachate onto single-walled carbon nanotubes (SWCNTs) was investigated. Different leachate solutions were prepared by altering the pH, ionic strength, and dissolved organic carbon (DOC) in the solutions to mimic the varying water conditions that occur in leachate during the various stages of waste decomposition. The youngest and oldest leachate solutions contained varying DOC and background chemistry and were represented by leachate Type A (pH = 5.0; DOC = 2500 mg/L; conductivity = 12,500 μS/cm; [Ca²⁺] = 1200 mg/L; [Mg²⁺] = 470 mg/L) and Type E (pH = 7.5; DOC = 250 mg/L; conductivity = 3250 μS/cm; [Ca²⁺] = 60 mg/L; [Mg²⁺] = 180 mg/L). These solutions were subsequently combined in different ratios to produce intermediate solutions, labeled B-D, to replicate time-dependent changes in leachate composition. Overall, a larger fraction of EE2 was removed as compared to BPA, consistent with its higher log K_OW value. The total removal of BPA and EE2 decreased in older leachate solutions, with the adsorptive capacity of SWCNTs decreasing in the order of leachate Type A > Type B > Type C > Type D > Type E. An increase in the pH from 3.5 to 11 decreased the adsorption of BPA by 22% in young leachate and by 10% in old leachate. The changes in pH did not affect the adsorption of EE2 in the young leachate, but did reduce adsorption by 32% in the old leachate. Adjusting the ionic strength using Na⁺ did not significantly impact adsorption, while increasing the concentration of Ca²⁺ resulted in a 12% increase in the adsorption of BPA and a 19% increase in the adsorption of EE2. DOC was revealed to be the most influential parameter in this study. In the presence of hydrophilic DOC, represented by glucose in this study, adsorption of the endocrine disrupting compounds (EDCs) onto the SWCNTs was not affected. In the absence of SWCNTs, hydrophobic DOC (i.e., humic acid) adsorbed 15-20% of BPA and EE2. However, when the humic acid and SWCNTs were both present, the overall adsorptive capacity of the SWCNTs was reduced. Hydrophobic (π-π electron donor-acceptor) interactions between the EDCs and the constituents in the leachate, as well as interactions between the SWCNTs and the EDCs, are proposed as potential adsorption mechanisms for BPA and EE2 onto SWCNTs.     

运用模糊聚类分析方法整编翰林桥站流量资料初探

主要对水文资料整编采用模糊聚类分析的方法进行尝试，利用计算机定线，使得水文资料整编全面计算机化作了初步的探索，取得一定的效果。     

THERMAL DEATH TIMES OF ESCHERICHIA COLI IN YOUNG COCONUT ENDOSPERM BEVERAGE

The decimal reduction times (D values) of Escherichia coli (American Type Culture Collection 25922) were established in a young coconut endosperm beverage, a famous local drink in the Philippines and in many tropical countries. Artificially inoculated cells were heated to 60, 70 and 80C at various heating times prior to survivor enumeration by surface plating onto pre-solidified Eosine Methylene Blue Agar. Results showed that the surviving populations significantly (P  <  0.05) decreased with increasing exposure time and temperature. The calculated D values ranged from 0.26  ±  0.01 to 0.56  ±  0.08   min. Validation of the results by establishing the thermal resistance of other E. coli isolates in the coconut beverage medium was recommended.

PRACTICAL APPLICATION

The study established the thermal inactivation rates of Escherichia coli (American Type Culture Collection 25922) in a young coconut endosperm beverage medium in various heating temperatures. The results obtained from this study may be used in the calculations of appropriate thermal process schedules for the test beverage against the test organism.     

STORAGE RESULTS IN LOSS OF THE ANTIGENOTOXIC PROPERTIES OF LENTINULA EDODES (SHIITAKE MUSHROOM) AND DEVELOPMENT OF IN VIVO GENOTOXICITY

In this study, we have investigated the stability of the antigenotoxic properties of dried of Lentinula edodes (Berk.) Singer mushroom powder stored at room and at − 20C. Mice were pretreated by gavage for 15 consecutive days with 0.6 mL/day of an aqueous extract of dried L. edodes powder prepared at 60C. On day 15, the mice were intraperitoneally injected with 50 mg/kg N-ethyl-N-nitrosourea (ENU); 3 h later, peripheral blood was withdrawn from the animals and used to evaluate DNA damage using the comet assay. The mice were sacrificed 24 h after the ENU treatment and the bone marrow used to measure the frequency of micronucleated polychromatic erythrocytes. The results indicated that storage at both room temperature and at − 20C significantly reduced the antigenotoxic effects of the mushroom powder. In addition, whereas freshly dried mushroom powder was nongenotoxic, storage of the powder at − 20C resulted in genotoxic activity ( P <  0.05). Taken together, our data indicate that constituents of L. edodes powder are altered during storage, resulting in both genotoxicity and a reduction in antigenotoxicity.