The yeast spindle pole body component Spc72p interacts with Stu2p and is required for proper microtubule assembly

We have previously shown that Stu2p is a microtubule-binding protein and a component of the Saccharomyces cerevisiae spindle pole body (SPB). Here we report the identification of Spc72p, a protein that interacts with Stu2p. Stu2p and Spc72p associate in the two-hybrid system and can be coimmunoprecipitated from yeast extracts. Stu2p and Spc72p also interact with themselves, suggesting the possibility of a multimeric Stu2p-Spc72p complex. Spc72p is an essential component of the SPB and is able to associate with a preexisting SPB, indicating that there is a dynamic exchange between soluble and SPB forms of Spc72p. Unlike Stu2p, Spc72p does not bind microtubules in vitro, and was not observed to localize along microtubules in vivo. A temperature-sensitive spc72 mutation causes defects in SPB morphology. In addition, most spc72 mutant cells lack cytoplasmic microtubules; the few cytoplasmic microtubules that are observed are excessively long, and some of these are unattached to the SPB. spc72 cells are able to duplicate and separate their SPBs to form a bipolar spindle, but spindle elongation and chromosome segregation rarely occur. The chromosome segregation block does not arrest the cell cycle; instead, spc72 cells undergo cytokinesis, producing aploid cells and polyploid cells that contain multiple SPBs.     

利用调和映射的复杂网格曲面螺旋刀轨生成算法

针对截平面法规划的复杂网格曲面刀轨的加工效率不高问题,提出一种复杂网格曲面螺旋刀轨生成算法.首先采用调和映射的方法对网格曲面进行参数化,然后根据残留高度确定参数网格中的参数环,在相邻参数环的参数点之间进行“分组匹配”,并依次计算初始和精确的对角参数螺旋线;在此基础上采用“区域划分”的方法快速生成了无干涉的网格曲面螺旋刀轨.对于复杂网格曲面的实验结果表明,文中的螺旋刀轨能够有效地提高截平面法刀轨的加工效率,且能够保证较好的加工质量.     

独立学院大学计算机基础课程教学的几点思考

针对当前独立学院大学计算机基础课程教学中面临的一些问题,结合教学实践,提出了分层教学模式,以期更好地为独立学院培养计算机技能上手快,后劲足的应用型人才:对不同层次的对象采取不同的教学内容和方式;面向专业,将计算机基础教学内容模块化,结合专业划分必修与选修模块,在内容深度和广度上更好地为专业服务;教学内容面向实用,兼顾体系,突出重点,精讲精练.     

观测永久丢失下随机离散事件系统故障预测的验证算法

针对随机离散事件系统在故障预测时可能出现系统观测永久丢失,导致预测不准确的问题,提出一种观测永久丢失下故障预测验证的算法。首先对观测永久丢失的随机离散事件系统的U-可预测性进行了形式化。其次使用随机预测器构造了一个随机离散事件系统的U-预测器,实现了系统的故障预测。基于U-预测器,提出了随机离散事件系统U-可预测性的充分必要条件及验证算法,并且引入成对的方式,明显地改进了该验证算法的复杂度。仿真结果表明,该验证算法使得观测永久丢失下系统故障预测准确。最后,实例说明观测永久丢失下故障预测验证算法的应用。结果表明,该验证算法相比现有同类验证算法应用范围更广,验证结果更精确。     

Application of genetic algorithm-based intuitionistic fuzzy weighted c-ordered-means algorithm to cluster analysis

Knowledge and Information Systems - With the advance of information technology, many fields have begun using data clustering to reveal data structures and obtain useful information. Most of the...     

校园网络立体化数据存储方案的研究与设计

本文针对校园网络数据存储方式单一、成本高、实用性不高的问题,设计了一个实用、可操作性的立体化数据存储方案。该方案发挥raid、虚拟存储和数据容灾三种存储技术各自的优势,将这三种存储技术有机的融合,构建了三个存储层,物理存储层、虚拟存储层、数据容灾备份层,每个层又采取两种不同的存储方式,在实用、低廉的基础上实现数据的安全冗余和立体化备份,从而确保校园应用数据的安全性和可用性。     

Optimal Performance in Tracking Stochastic Signal Under Disturbance Rejection

The analysis method of optimal tracking performance is proposed for multiple‐input multiple‐output (MIMO) linear time‐invariant (LTI) systems under disturbance rejection. An H₂ criterion of the error signal between the output of the plant and the reference signal is used as a measure for the tracking performance. Spectral factorization is applied to obtain the optimal solution of the system tracking error. The explicit expressions are derived for this minimal tracking error with respect to random reference signals under disturbance rejection. It is shown that the nonminimum phase zeros, the zero direction, the unstable poles, the pole direction of a given plant, statistical characteristics of the reference input signal, and disturbance signal have a negative effect on a feedback system's ability to reduce the system error with disturbance rejection. The results show that the optimal tracking performance will further be damaged because of disturbance rejection. Some typical examples are given to illustrate the theoretical results.     

基于数字媒体技术的图像融合算法

图像融合技术是数字媒体技术中很重要的一个方向。图像融合因为其融合后可以保留相对较多的场景,融合后图像精度高,便于后续图像中目标观测和特征点的提取。研究一种基于数字媒体技术的图像融合算法,并与其它传统的图像融合算法进行了比较。