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51.
A method to determine the refractive index and thickness of silver halide emulsions used in holography is presented. The emulsions are in the form of a layer of film deposited on a thick glass plate. The experimental reflectances of p-polarized light are measured as a function of the incident angles, and the values of refractive index, thickness, and extinction coefficient of the emulsion are obtained by using the theoretical equation for reflectance. As examples, five commercial holographic silver halide emulsions are analyzed. The procedure to obtain the measurements and the numerical analysis of the experimental data are simple, and agreement of the calculated reflectances, by use of the thickness and refractive index obtained, with the measured reflectances is satisfactory. 相似文献
52.
F Jurado JM Bellón JA Pareja A Golitsin L Millán G Pascual J Buján 《Canadian Metallurgical Quarterly》1998,13(3):761-774
The present study investigates the effects on the cardiac muscle cell of two of the determining factors for the success of organ transplant; ischaemia-perfusion and immunosuppressive treatment with cyclosporin-A (CsA). To this end an abdominal, heterotopic heart transplant model in singenic Sprague-Dawley rats was employed. Three study groups were established: Group I (control, n = 15) animals undergoing heart transplant without treatment; Group II (n = 15) animals undergoing heart transplant and subjected to a daily dose of CsA in a cremophor vehicle (Sandimun) (5 mg/kg/sc); Group III (n = 15): animals undergoing heart transplant and administered a daily dose of pure CsA (5 mg/kg/sc). Recipient animals were sacrificed 7, 14, 21, 30 and 50 days after transplant. During the post-operative period, heart function was assessed by daily abdominal palpation. Graft specimens obtained at each follow-up period were subjected to light and transmission electron microscopy. Immunohistochemical analysis of specimens was performed using the rat macrophage-specific monoclonal antibody MCA-341. The ischaemia/reperfusion process induced considerable alteration to cardiac muscle cells of control animals. Effects, apparent after the first week of transplant, included mitochondrial swelling and loss of cristae, hypertrophy of the sarcoplasmic reticulum and structural changes to sarcomeres. Two weeks after transplant, the myocardium was infiltrated by inflammatory cells. These effects diminished 30 days post-transplant. Cardiac tissues of treated animals (groups II and III) showed similar behaviour although, in the latter group, mitochondrial damage was greater and intense myocardial fibrosis took place. Infiltration of cardiac muscle by white blood cells did not take place until 3 weeks post-implant. These results indicate: a) The ultrastructural changes detected in cardiac fibres of animals of the three study groups were attributable to the ischaemia/reperfusion process rather than to treatment with CsA; b) CsA appears to augment mitochondrial damage and myocardial fibrosis; c) the inflammatory response was delayed and reduced by the immunosupressant; and d) the cremophor administration vehicle did not seem to exert an independent toxic effect on the myocardium. 相似文献
53.
JM Roda F Carceller JM Pascual MJ Herguido F González-Llanos M Alonso de Leci?ana C Avenda?o E Díez-Tejedor 《Canadian Metallurgical Quarterly》1998,13(9):427-430
This paper describes the potential markers of cell death and connective tissue degradation which might serve as markers of periodontal disease activity. The first section deals with enzymes released by dead and degenerating cells. Firstly, it describes how these pass from the periodontal tissues into gingival crevicular fluid (GCF) and explains that these enzymes have been used as markers of cell death in medicine for several decades. It then discusses the main enzymes in this group, aspartate amino transferase (AST) and lactate dehydrogenase (LDH) and reviews those studies which have attempted to relate these enzymes to periodontal disease severity and activity. Secondly, it describes the potential markers of connective tissue degradation, fibronectin, hydroxyproline-containing peptides and glycosaminoglycans (GAGs) and explains how these are produced. Finally, it describes the only commercial test kit for markers in this group (GCF-AST). 相似文献
54.
55.
The expression of the beta-amyloid precursor protein (APP), which plays a key role in the development of Alzheimer's disease, is regulated by a variety of cellular mediators in a cell-dependent manner. In the present study, we present evidence that thyroid hormones negatively regulate the expression of the APP gene in neuroblastoma cells. Transient transfection studies using plasmids that contain progressive deletions of the 5' region of the gene demonstrate that triiodothyronine (T3), the more active form of the thyroid hormones, represses APP promoter activity by a mechanism that requires binding of the nuclear T3 receptor (TR) to a specific sequence located in the first exon. The unliganded receptor increases promoter activity, and T3 reverses that activity to basal levels. The repressive effect of T3 does not exhibit TR isoform specificity, and it is equally mediated by TRalpha and TRbeta. Gel mobility shift assays using in vitro synthesized nuclear receptors and nuclear extracts led to the identification of a negative thyroid hormone response element, at nucleotide position +80/+96, that preferentially binds heterodimers of TR with the retinoid X receptor. Insertion of sequences containing this element confers negative regulation by T3 to a heterologous TK promoter, thus indicating the functionality of the element. 相似文献
56.
The phase diagram for the system ZrO2-Er2O3 was redetermined. At high temperatures, the system is dominated by wide regions of solid solution based on ZrO2 and Er2O3 separated by a two-phase field which appears to extend to the solidus. The range of existence of these solid solution fields was determined using the precision lattice parameter method. A low-temperature (<1800° C) long-range ordering occurred between 20 and 90 mol % Er2O3, and three ordered phases were found: the first compound was present at 40 mol % Er2O3 and corresponds to the ideal formula Er4Zr3O12 with rhombohedral symmetry (space group R¯3), is isostructural with UY6O12, and decomposes at about 1500° C into fluorite solid solution by an order-disorder process; the second ordered phase is formed at about 55 mol % Er2O3, its formula is close to Er5Zr2O11.5, and it decomposes at about 1650° C into cubic solid solutions of the fluorite and C-type; the third compound is formed at 75 mol % Er2O3, its formula is Er6ZrO11, it has a wide homogeneity range, and it decomposes above 1700° C into a cubic solid solution of the C-type. Liquidus determination indicated the existence of a peritectic at 62 mol % Er2O3.This work is based on the Ph.D. thesis of C. Pascual, Madrid University, 1980. 相似文献
57.
Lorente N Pascual JI 《Philosophical transactions. Series A, Mathematical, physical, and engineering sciences》2004,362(1819):1227-1238
Inelastic tunnelling electrons are a proper excitation source to induce chemical transformations on a single adsorbate. When their energy is tuned to that of molecular vibrational states, the modification may follow complex internal vibrational pathways. Here, we analyse our recent results on the selective excitation of ammonium stretching or bending modes to control the outcome of a simple bond-cleavage reaction. With the help of model calculations, we provide a detailed molecular-scale picture of the competing internal pathways leading to molecular movement. A mode-selective strategy, based on local excitations of specific reaction coordinates, has an important drawback when applied to adsorbate systems due to the problem of fast energy randomization. The success of such a mode-selective strategy is determined here by the ability of the scanning tunnelling microscope to study reactivity in the limit of very low yield and very low power irradiation, in a regime where vibrational heating of the adsorbate/surface system becomes negligible. 相似文献
58.
Laureano?LambánEmail author Vico?Pascual Julio?Rubio 《Applicable Algebra in Engineering, Communication and Computing》2003,14(3):187-215
In a previous paper we characterized, in the Category Theory setting, a class of implementations of Abstract Data Types, which has been suggested by the way of programming in the EAT system. (EAT, Effective Algebraic Topology, is one of Sergeraerts systems for effective homology and homotopy computation.) This characterization was established using classical tools, in an unrelated way to the current mainstream topics in the field of Algebraic Specifications. Looking for a connection with these topics, we have found, rather unexpectedly, that our approach is related to some object-oriented formalisms, namely hidden specifications and the coalgebraic view. In this paper, we explore these relations making explicit the implicit object-oriented features of the EAT system and generalizing the data structure analysis we had previously done. 相似文献
59.
María?D.?GuillénEmail author Ainhoa?Ruiz Nerea?Cabo Rosana?Chirinos Gloria?Pascual 《Journal of the American Oil Chemists' Society》2003,80(8):755-762
Three oil samples obtained from sacha inchi (Plukenetia volubilis L.) seeds were studied by means of FTIR and 1H NMR. Frequency data of the most significant bands of the IR spectrum of this oil are given. These data show that sacha inchi
oil has a high degree of unsaturation. The same fact is deduced from the ratio between the absorbance of the bands due to
the stretching vibrations of the cis olefinic CH double bonds at 3010.5 cm−1 and to the methylene symmetrical stretching vibrations at 2855.1 cm−1. The proportions of monounsaturated, polyunsaturated, and saturated acyl groups were predicted from the frequency of some
IR bands, and these were in satisfactory agreement with the values obtained through FAME generation and their quantification
by GC. Likewise, simple observation of the 1H NMR spectra provided a great deal of information about sacha inchi oil, with regard not only to the relative proportions
of the different acyl groups but also to their nature. Thus, the presence of γ-linolenic acyl groups was discounted. Furthermore,
the area of some 1H NMR signals was used to determine the proportion of saturated and mono-, di-, and triunsaturated acyl groups, which also
were in satisfactory agreement with the values obtained by classical methods. IR and 1H NMR determinations take very little time in comparison with classical methods and do not require chemical manipulation or
transformation of the sample. A comparison was also made between the compositions of sacha inchi and linseed oil. Both oils
are important sources of the healthful n−3 linolenic acyl groups, and sacha inchi also contains high proportions of the n−6
linoleic acyl groups. 相似文献
60.
The mechanism for hologram formation by fixation-free rehalogenating bleaching has been treated by several authors. Experiments carried out with Kodak and Agfa photographic plates have led to the conclusion that the transfer of material from the exposed to the unexposed zones is the main mechanism that drives the process. We show that when BB-640 emulsion is used in the recording of holograms by means of fixation-free rehalogenating bleaching, the mechanism for the transfer of material from exposed to unexposed zones is efficient enough to create high refractive-index modulations. We also comment on the overmodulation effects, which result in a decrease in diffraction efficiency. 相似文献