Sex differences in children's response to training on a visual-spatial test.

In a study with 133 children in Grades 1, 3, and 5, a brief training procedure designed to improve performance on the Children's Embedded Figures Test, a test of visual ability, resulted in significant improvement for girls but not for boys. No sex difference was observed among children receiving this training. The implications for theories of sex differences in visual–spatial ability are discussed. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Identification of a neurosteroid binding site contained within the GluR2-S1S2 domain

Glutamate receptors play a major role in neural cell plasticity, growth, and maturation. The degree to which ionotropic glutamate receptors (iGluR) conduct current is dependent on binding of extracellular ligands, of which glutamate is the native agonist. Although the glutamate binding site of the GluR2 class of amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) iGluR has been structurally characterized, the allosteric sites attributed to neurosteroid binding have yet to be localized. Here, using intrinsic tryptophan fluorescence spectroscopy, we show that the extracellular glutamate binding core of the GluR2 class of AMPA receptors also binds to two neurosteroids, pregnenolone sulfate (PS) and 3α-hydroxy-5β-pregnan-20-one sulfate, both of which negatively modulate its activity. Interest in these sulfated neurosteroids stems from their differential modulation of other members of the iGluR family and their potential use as endogeneous agents for stroke therapy. In particular, whereas PS inhibits AMPA and other non-N-methyl-d-aspartate (NMDA) family members, it activates the NMDA receptor. In addition to providing evidence for binding of these neurosteroids to the glutamate binding core of the GluR2 class of AMPA receptors, our data suggests that both neurosteroids bind in a similar manner, consistent with their modulation of activity of this class of iGluR. Interestingly, the conformational change induced upon binding of these neurosteroids is distinct from that induced upon glutamate binding.     

Quantatitive assessment of enzyme immobilization capacity in porous silicon

Immobilized enzyme systems are important in a broad range of applications, from biological sensing to the industrial-scale biocatalytic synthesis of chiral products. We demonstrate the ability to systematically vary and quantitatively assess the immobilization capacity of porous silicon thin films for the enzyme glutathione-S-transferase in a manner predicted by a simple geometric model of the porous silicon matrix. We find that the immobilization capacity quantatitively correlates with systematic changes in the device thickness. These results are significant since, despite the wide range over which porous silicon morphology and surface area can be varied, few attempts have been made to systematically characterize surface binding capacity. Our findings suggest that porous silicon can be an ideal matrix, where immobilization of a predictable quantity of biological material is desired.     

The Emotional Wardrobe

Since the industrial revolution, fashion and technology have been linked through the textile and manufacturing industries, a relationship that has propelled technical innovation and aesthetic and social change. Today, a new alliance is emerging through the integration of electronic technology and smart materials on the body. This study addresses the integration of technology with clothing from a fashion perspective, and examines its expressive and interactive potential. It proposes the concept of The Emotional Wardrobe: clothing that represents and stimulates emotional response through the interface of technology. It asks if fashion can offer a more personal and provocative definition of self, which actively involves the wearer in a mutable aesthetic identity. A multi-disciplinary framework combines fashion, material science and the real-time, affective computing platform, called AffectiveWare. By merging technology and fashion, The Emotional Wardrobe becomes a poetic interface, shifting emphasis from human–computer interaction to computer-aided, human–human communication.     

Counselor hypothesis-testing strategies.

Conducted 2 experiments relevant to the questioning strategies counselors use in testing their hypotheses about clients. In Exp I, 60 undergraduates were asked to select 12 questions from a list of 38 that would be most helpful in getting to know someone. Ss were asked to test a hypothesis that the other person was an extravert or introvert. The questions were categorized by the experimenters as extraverted, introverted, unbiased, or irrelevant. Most Ss selected a strategy that favored unbiased questions over biased ones. In Exp II, 40 Ss played the roles of clients, and 40 advanced doctoral students in counseling or clinical psychology played the roles of counselors. Counselors were advised that they would soon meet with their client to test a hypothesis that the client possessed or lacked self-control. Counselors were instructed to compose 10 questions to ask the client. The actual interviews were then recorded. The frequency with which counselors developed questions that were confirmatory, disconfirmatory, irrelevant, or unbiased in regard to the hypothesis was not influenced by the specific hypothesis. Results of both experiments do not confirm the hypothesis that counselors preferentially seek information to confirm hypotheses about clients. (21 ref) (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Racial and attitudinal factors affecting the perceptions of counselors by Black adolescents.

Presented to 247 Black male and female high school students racial and attitudinal information about a hypothetical male or female counselor and asked them to express their perceptions of the counselor. Attitudinal information about a counselor had a stronger effect than racial information on Ss' perception of the counselor: Counselors portrayed as attitudinally similar were rated significantly higher in attractiveness, trustworthiness, expertness, and social attraction than those portrayed as attitudinally dissimilar. Racial information also influenced perceived attractiveness: White counselors were rated higher than Black ones in attractiveness, although there was no difference in ratings of trustworthiness or expertise. White female counselors were perceived as more expert than their Black female counterparts, whereas the ratings of male counselors were not influenced by the racial variable. Implications for counselor–client relationships and the development of mental health services for minority populations are discussed. (33 ref) (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Specificity is complex and time consuming: mutual exclusivity in tyrosine kinase-mediated signaling

Most fundamental cellular processes are transduced through tyrosine kinase (TK)-mediated pathways. For transduction without corruption, the protein-protein interactions involved have to be mutually exclusive. Many of these proteins bind via homologous domains whose binding characteristics suggest that their innate specificity is not sufficiently high to account for the integrity of signal transduction. Stimulation of TK-mediated signals is often accompanied by recruitment of a precise, multimolecular protein complex that is itself capable of imposing specificity. Furthermore, this complex provides protection against phosphatase activity, controlling the longevity of the active signaling complex, and thus influencing outcomes in subsequent downstream events.     

Application of the Cytosensor microphysiometer to drug discovery

The Cytosensor microphysiometer uses silicon chip technology to correlate changes in extracellular acidification rates with quantitative changes in cellular metabolism in response to ligand binding to surface receptors. This functional measure of physiology makes the Cytosensor a valuable tool in drug discovery research by allowing application of the instrument to screening of prospective pharmacologically active agents, characterizations of dose responses and structure-activity relationships, and investigation of mechanisms of action.     

Biased processing of emotional information in girls at risk for depression.

Researchers have documented that children of depressed mothers are at elevated risk for developing a depressive disorder themselves. There is currently little understanding, however, of what factors place these children at elevated risk. In the present study, the authors investigated whether never-disordered daughters whose mothers have experienced recurrent episodes of depression during their daughters' lifetime are characterized by biased processing of emotional information. Following a negative mood induction, participants completed an emotional-faces dot-probe task. Daughters at elevated risk for depression, but not control daughters of never-disordered mothers, selectively attended to negative facial expressions. In contrast, only control daughters selectively attended to positive facial expressions. These results provide support for cognitive vulnerability models of depression. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Adhesive forces significantly affect elastic modulus determination of soft polymeric materials in nanoindentation

The present study investigated the effects of adhesion on the elastic modulus determined from nanoindentation curves for soft polydimethylsiloxane (PDMS) elastomers with five different crosslink concentrations. Indentation load-displacement curves were obtained for samples of all concentrations at four different peak loads. All load-displacement curves were nearly linear, resulting in load independent contact stiffnesses (p < 0.003) for the range of loads tested. As a result, elastic modulus calculated from nanoindentation curves with the Hertz contact model exhibited significant differences (p < 0.004) both at different peak loads for a single PDMS concentration and between different PDMS concentrations at a single peak load (p < 0.001). The differences for different peak loads were attributed to the presence of substantial adhesive forces at the tip-sample interface. By taking these adhesive interactions into account with the Johnson, Kendall, Roberts (JKR) contact model, the differences in elastic modulus at different peak loads could be reconciled. Significant differences (p < 0.001) in moduli between different PDMS concentrations were still present. The results highlight the importance of considering adhesive forces in nanoindentation analyses of low modulus polymeric materials.