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121.
We show here for the first time that a stable parallel double helix with Hoogsteen pairing can exist independently of the triple helix of which it is a component part. The experiments employ DNA oligonucleotides with mixed sequences of normal bases. These duplexes are distinct from previously reported ribopolynucleotide helices containing bulky substituents which prevent Watson-Crick pairing as well as from parallel duplexes with Donohue, or reversed Watson-Crick, pairing. Stoichiometry is established by mixing curves and gel electrophoresis. Tm depends linearly upon pH, increasing with acidity because of the need to protonate N3 of C. The Tm of the 20-mer studied here is 52 degrees C at pH 5.2 and 0.1 M NaCl. At pH above 6, the molecule rearranges to form an antiparallel duplex with imperfect Watson-Crick pairing and loops, and the Tm is then independent of pH. The CD spectrum of the parallel duplex is very similar to that of the corresponding triple helix but quite different from that of the Watson-Crick helix. The infrared spectrum in the double bond region closely resembles that of the triple helix but, as with the CD, is quite different from that of the Watson-Crick duplex. The infrared spectra of the duplex and triple helix are also nearly identical in the region form 800 to 1000 cm-1, which is sensitive to backbone conformation. The only symmetry element present is a pseudorotational axis coincident with the helix axis of the parallel duplex as well as with the axis of the corresponding triple helix.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
122.
Tian-Hua Liu 《Industrial Electronics, IEEE Transactions on》1995,42(1):17-24
This paper presents a new method to achieve a maximum torque for a single-phase induction motor. An AC adjustable capacitor using an electronic switch in parallel with a capacitor is proposed. The capacitor is short-circuited in a different period by an electronic switch during each cycle to vary the effective value of the AC capacitor. Two new optimization algorithms, which obtain a maximum starting torque by adjusting the effective capacitor, are proposed. No starting capacitor or centrifugal switch is used here. A theoretical analysis, and simulated and experimental results are presented in this paper 相似文献
123.
RoleofREandMgonGraphitizationofSpheroidalGraphiteMalleableCastIron¥LiuJin-Hai(HebeiInstituteofTechnotogy,Mianjin300130,China)... 相似文献
124.
The changes in the intestinal mucosal permeability were observed by quantitatively assessing plasma to luminal clearance of 99mTc-labeled DTPA, and the influence of platelet activating factor (PAF) on it was investigated. The results showed that intestinal permeability was significantly elevated after severe burn and was positively correlated with increase in PAF in the intestinal tissue (r = 0.94, P < 0.01). PAF antagonist therapy could significantly attenuate postburn intestinal mucosal permeability. It is concluded that PAF is one of the important factors causing increased intestinal permeability after severe burn. 相似文献
125.
Microorganisms capable of degrading di-n-butyl phthalate (DBP) were isolated. The characteristics of DBP biodegradation by immobilized and free cells were investigated. The experimental results showed that the rate of DBP degradation of immobilized cells was higher than that of free cells. 相似文献
126.
Chun Hu Ji Zhao Li G.P. Liu P. Worley E. White J. Kjar R. 《Electron Device Letters, IEEE》1995,16(2):61-63
The effects of the plasma etching process induced gate oxide damages on device's low frequency noise behavior are investigated on MOSFET's fabricated with different field plate perimeter to gate area ratio antennas. Abnormal 1/f noise spectrum with a shoulder centered in the frequency range of 100 and to 1 kHz was frequently observed in small geometry devices, and it is attributable to a nonuniform distribution of oxide traps induced by plasma etching process 相似文献
127.
128.
ElectrochemicalInvestigationontheFormationofDyFeAloyinMoltenChloridesLiuGuankun(刘冠昆),TongYexiang(童叶翔),HongHuichan(洪惠婵),Yang... 相似文献
129.
Two kinds of biosensors for amino acids (one with nonspecific enzyme as bioelement, the other with specific enzyme(s) as bioelement),
including their principles, applications, recent researches and future trends were discussed in detail. 61 references were
given.
A part of work for gaining Ph. D in chemical and biochemical application at FPMs (Mons, Belgique)
Synopsis of the first author Xia Jinlan, Male, born in 1963, now at Facult’e Polytechnique de Mons, Belgique 相似文献
130.
Liu Y. Blostein S.D. 《IEEE transactions on information theory / Professional Technical Group on Information Theory》1992,38(1):177-182
Bayesian analysis is used to show that Wald's sequential probability ratio test with varying thresholds is optimal for the nonstationary situation, where the observed samples are independent but not identically distributed. Some important properties useful for the design of the test thresholds are discussed. Wald's lower bound, generalized to the nonstationary situation, is also presented. The results have important applications in situations where the observed signal is time-varying. such as in radar signal processing, image processing, and spread spectrum communications 相似文献