Unipolar plasma electrolytic oxidation: Waveform optimisation for corrosion resistance of commercially pure titanium

This study deals with the anodisation of titanium grade 2 in 0.5-M sulphuric acid using a pulsed signal in a unipolar regime. The electrical parameters investigated are voltage, frequency and duty cycle. The use of duty cycles with a high percentage of anodic polarisation (90%), combined with high frequencies (1000 Hz) and the higher voltage tested (220 V), favoured the establishment of a plasma regime involving strong dielectric discharges, allowing the growth of thicker oxides but with rough architecture. The corrosion resistance of the formed film has been characterised by potentiodynamic tests in 0.5-M NaBr for localised corrosion resistance and by immersion tests in 10% v/v sulphuric acid solution for a uniform corrosion assessment. Current–time curves, visual observations and electron microscope analysis (scanning electron microscopy, energy-dispersive X-ray spectroscopy) were the tools selected to provide a correlation between technological parameters and oxide growth mechanism. For localised and uniform corrosion, anodisation at 220 V with a high level of anodic polarisation (90%) and frequency (1000 Hz) was verified to be particularly advantageous.     

Synergy in Cystic Fibrosis Therapies: Targeting SLC26A9

SLC26A9, a constitutively active Cl⁻ transporter, has gained interest over the past years as a relevant disease modifier in several respiratory disorders including Cystic Fibrosis (CF), asthma, and non-CF bronchiectasis. SLC26A9 contributes to epithelial Cl⁻ secretion, thus preventing mucus obstruction under inflammatory conditions. Additionally, SLC26A9 was identified as a CF gene modifier, and its polymorphisms were shown to correlate with the response to drugs modulating CFTR, the defective protein in CF. Here, we aimed to investigate the relationship between SLC26A9 and CFTR, and its role in CF pathogenesis. Our data show that SLC26A9 expression contributes to enhanced CFTR expression and function. While knocking-down SLC26A9 in human bronchial cells leads to lower wt- and F508del-CFTR expression, function, and response to CFTR correctors, the opposite occurs upon its overexpression, highlighting SLC26A9 relevance for CF. Accordingly, F508del-CFTR rescue by the most efficient correctors available is further enhanced by increasing SLC26A9 expression. Interestingly, SLC26A9 overexpression does not increase the PM expression of non-F508del CFTR traffic mutants, namely those unresponsive to corrector drugs. Altogether, our data indicate that SLC26A9 stabilizes CFTR at the ER level and that the efficacy of CFTR modulator drugs may be further enhanced by increasing its expression.     

Current advances concerning the most cited metal ions doped bioceramics and silicate-based bioactive glasses for bone tissue engineering

Studies related to biomaterials that stimulate the repair of living tissue have increased considerably, improving the quality of many people's lives that require surgery due to traumatic accidents, bone diseases, bone defects, and reconstructions. Among these biomaterials, bioceramics and bioactive glasses (BGs) have proved to be suitable for coating materials, cement, scaffolds, and nanoparticles, once they present good biocompatibility and degradability, able to generate osteoconduction on the surrounding tissue. However, the role of biomaterials in hard tissue engineering is not restricted to a structural replacement or for guiding tissue regeneration. Nowadays, it is expected that biomaterials develop a multifunctional role when implanted, orchestrating the process of tissue regeneration and providing to the body the capacity to heal itself. In this way, the incorporation of specific metal ions in bioceramics and BGs structure, including magnesium, silver, strontium, lithium, copper, iron, zinc, cobalt, and manganese are currently receiving enhanced interest as biomaterials for biomedical applications. When an ion is incorporated into the bioceramic structure, a new category of material is created, which has several unique properties that overcome the disadvantages of primitive material and favors its use in different biomedical applications. The doping can enhance handling properties, angiogenic and osteogenic performance, and antimicrobial activity. Therefore, this review aims to summarize the effect of selected metal ion dopants into bioceramics and silicate-based BGs in bone tissue engineering. Furthermore, new applications for doped bioceramics and BGs are highlighted, including cancer treatment and drug delivery.     

The importance of landscape in wine quality perception: An integrated approach using choice-based conjoint analysis and combination-based permutation tests

This work’s aim is to evaluate the importance of landscape in wine quality perception using a novel integrated approach based on two statistical techniques, i.e. choice-based conjoint analysis (Gustafsson et al., 2001) and combination-based permutation tests (Corain and Salmaso, 2004, Pesarin and Salmaso, 2009a). To this end we developed a preference choice experiment which was submitted to a sample of respondents during blind wine tasting meetings. The obtained results allow us to weigh up and emphasise the importance of an attractive landscape to the perception of a finer quality of wine. Indeed, we noticed that associating wine to an image of greater visual impact, i.e. an evocative landscape, induces a significantly higher preference for the tasted wine. Moreover, the integrated application of choice-based conjoint analysis and combination-based permutation tests allows us to identify the specific relationship each market segment has with landscape, price and wine quality.     

To swim or to jump? Passage behaviour of a potamodromous cyprinid over an experimental broad‐crested weir

Physical stressors, such as man‐made obstacles, are considered one of the main causes that negatively affect freshwater fish. Even small weirs may impact fish populations, including potamodromous cyprinids, by partially or totally blocking upstream migratory movements. Some studies have addressed the effect of key hydraulic parameters on upstream movements past small weirs, but little is known on how these parameters interact to induce swimming or jumping behaviour in negotiating such obstacles. This study aims to evaluate the passage behaviour (swimming vs. jumping) of Iberian barbel (Luciobarbus bocagei), a potamodromous species, over an experimental small broad‐crested weir, considering the effect of different plunge pool depths (D), waterfall heights (H), and weir discharges (Q). Results revealed that passage behaviour was highly dependent on combinations of plunge pool depths and waterfall heights. Barbel navigated most configurations by swimming (overall outcomes: 81.5% swimming vs. 18.5% jumping), except the ones with the higher waterfall heights (25 cm) tested. Therefore, higher waterfalls proved to be preponderant in the switching of passage behaviour from swimming to jumping. Regarding the discharge over the 1‐m‐wide weir (overall outcomes: 85% swimming vs. 15% jumping), contrary to what was expected, there was no evidence that passage behaviour was discharge‐related, for the range of discharges tested (25–100 L/s). These results are useful to identify potential migration obstacles and should be taken into consideration in river restoration projects and when designing fishways for potamodromous cyprinids.     

Enzymatic synthesis of sugar esters and their potential as surface-active stabilizers of coconut milk emulsions

Sugar esters are compounds with surfactant properties (biosurfactants), i.e., capable of reducing the surface tension and promote the emulsification of immiscible liquids. On the other hand, as with all emulsions, coconut milk is not physically stable and is prone to phase separation. Therefore, the aim of this work was to evaluate the synthesis of fructose, sucrose and lactose esters from the corresponding sugars using Candida antarctica type B lipase immobilized in two different supports, namely acrylic resin and chitosan, and evaluate its application in the stabilization of coconut milk emulsions. The enzyme immobilized on chitosan showed the highest yield of lactose ester production (84.1%). Additionally, the production of fructose ester was found to be higher for the enzyme immobilized on the acrylic resin support (74.3%) as compared with the one immobilized on chitosan (70.1%). The same trend was observed for the sucrose ester, although with lower percentage yields. Sugar esters were then added to samples of fresh coconut milk and characterized according to their surface tension, emulsification index and particle size distribution. Although the microscopic analysis showed similar results for all sugar esters, results indicated lactose ester as the best biosurfactant, with a surface tension of 38.0 N/m and an emulsification index of 54.1%, when used in a ratio of 1:10 (biosurfactant:coconut milk, v/v) for 48 h experiments.     

Three-dimensional analysis of boreholes considering spatial variability of properties and poroelastoplasticity

In general, analysis of oil producing wells is carried out considering that both hydraulic and mechanical parameters of the rock mass are deterministic. Mechanical and hydraulic properties of rock masses and in particular sedimentary rock masses may show a considerable degree of spatial variability. This paper focuses on the evaluation of the three-dimensional borehole response, particularly of the plastic zone, taking into account the spatial variability of both hydraulic and mechanical properties. The analysis is performed with a developed finite element program that incorporates spatial variability, coupled fluid-mechanical effects and elastoplastic behavior of the rock mass. Examples are shown and conclusions are drawn regarding the effect of spatial variability on the three-dimensional borehole analysis.     

Auction procedures and competition in public services: The case of urban public transport in France and London

In many countries, governments are pushing for the introduction of competition in the organization of public services and more broadly in public procurement. The development of public–private partnerships throughout the world is a good illustration of this trend. In order to foster competition, competitive tendering through the use of auctions is now common. Nevertheless, competition for the field must be organized. Depending on the rules of the game chosen, introducing competition for the field may or may not be successful. In this paper we investigate two alternative models for organizing local public services, namely the French and the London models of urban public transport. Few competitors and collusive behaviours, with increasing costs, characterize the French model, while the London model, as far as we have seen, exhibits better results, by using the transparency of auction procedures and the discretionary power of the regulator as two complementary instruments to foster competition and prevent anti-competitive behaviours.     

Investigation of maximum doses absorbed by people accompanying patients in nuclear medicine departments

A recent EC directive has called for all member states to introduce legislation covering the assessment and restriction of air crew exposure to cosmic radiation. In the UK the Civil Aviation Authority, in conjunction with the Department of the Environment. Transport and the Regions issued guidelines suggesting the use of a predictive code such as CARI for this purpose. In order to validate the use of calculated route doses, an extensive programme of measurements is being carried out on long haul routes in conjunction with Virgin Atlantic Airways, using a prototype HAWK TEPC developed by Far West Technology. This programme began in January 2000 and by the end of February 2001 had resulted in the accumulation of data from 74 flights. In this paper the instrument design is discussed, together with the calibration programme. An overview of the in-flight results is also presented, including comparisons between measurements and calculations, which indicates that CARI under-predicts the route doses by approximately 20%.     

The excitation power density effect on the Si nanocrystals photoluminescence

In the present work we have studied the photoluminescence (PL) behavior from Si nanocrystals (NCs) as a function of the excitation power density and annealing time. The NCs were produced in a SiO₂ matrix by Si implantations from room temperature (RT) up to 700 °C, followed by post-annealing in N₂ atmosphere at high temperature. With this aim we have changed the excitation power density (from 2 × 10⁻³ W/cm² up to 15 W/cm²) and the annealing time (from 10 min up to 15 h). The strong PL signal, which at 15 W/cm² is composed by a single-peak structure (650–1000 nm) centered at around 780 nm, expands up to 1200 nm showing a two-peak structure when measured at 20 × 10⁻³ W/cm². The peak structure located at the short wavelength side is kept at 780 nm, while the second peak, starting at around 900 nm, redshifts and increases its intensity with the implantation temperature and annealing time. The effect of the annealing time on the PL spectra behavior measured at low excitation power agrees by the first time with the Si NC growth according to quantum confinement effects.