The Divergent Immunomodulatory Effects of Short Chain Fatty Acids and Medium Chain Fatty Acids

Fatty acids are derived from diet and fermentative processes by the intestinal flora. Two to five carbon chain fatty acids, termed short chain fatty acids (SCFA) are increasingly recognized to play a role in intestinal homeostasis. However, the characteristics of slightly longer 6 to 10 carbon, medium chain fatty acids (MCFA), derived primarily from diet, are less understood. Here, we demonstrated that SCFA and MCFA have divergent immunomodulatory propensities. SCFA down-attenuated host pro-inflammatory IL-1β, IL-6, and TNFα response predominantly through the TLR4 pathway, whereas MCFA augmented inflammation through TLR2. Butyric (C4) and decanoic (C10) acid displayed most potent modulatory effects within the SCFA and MCFA, respectively. Reduction in TRAF3, IRF3 and TRAF6 expression were observed with butyric acid. Decanoic acid induced up-regulation of GPR84 and PPARγ and altered HIF-1α/HIF-2α ratio. These variant immune characteristics of the fatty acids which differ by just several carbon atoms may be attributable to their origins, with SCFA being primarily endogenous and playing a physiological role, and MCFA exogenously from the diet.     

Association of IgG1 Antibody Clearance with FcγRIIA Polymorphism and Platelet Count in Infliximab-Treated Patients

The FcγRIIA/CD32A is mainly expressed on platelets, myeloid and several endothelial cells. Its affinity is considered insufficient for allowing significant binding of monomeric IgG, while its H131R polymorphism (histidine > arginine at position 131) influences affinity for multimeric IgG2. Platelet FcγRIIA has been reported to contribute to IgG-containing immune-complexe clearance. Given our finding that platelet FcγRIIA actually binds monomeric IgG, we investigated the role of platelets and FcγRIIA in IgG antibody elimination. We used pharmacokinetics analysis of infliximab (IgG1) in individuals with controlled Crohn’s disease. The influence of platelet count and FcγRIIA polymorphism was quantified by multivariate linear modelling. The infliximab half-life increased with R allele number (13.2, 14.4 and 15.6 days for HH, HR and RR patients, respectively). It decreased with increasing platelet count in R carriers: from ≈20 days (RR) and ≈17 days (HR) at 150 × 10⁹/L, respectively, to ≈13 days (both HR and RR) at 350 × 10⁹/L. Moreover, a flow cytometry assay showed that infliximab and monomeric IgG1 bound efficiently to platelet FcγRIIA H and R allotypes, whereas panitumumab and IgG2 bound poorly to the latter. We propose that infliximab (and presumably any IgG1 antibody) elimination is partly due to an unappreciated mechanism dependent on binding to platelet FcγRIIA, which is probably tuned by its affinity for IgG2.     

A THERMOMECHANICAL MODEL OF SOLIDIFICATION ON A MOLD MOVING WITH CONSTANT VELOCITY

A thermomechanical model of pure metal solidification on a moving mold plate is considered. The goal of the model is to obtain a formula for the contact pressure at the shell/mold interface as the mold moves into the molten liquid. From the contact pressure it is possible to infer the effects of the mold velocity and the mold microgeometry on the time and location of gap nucleation which results from irregular distortion of the shell as it grows from the melt. The mold, which moves at a constant velocity into the molten liquid, has a sinusoidal surface with a low aspect ratio: this means that its wavelength greatly exceeds its amplitude. The mold is of infinite area and is assumed to be perfectly conducting and thermomechanically rigid. We therefore neglect the complexities associated with the physics of edge constraints and/or free boundaries of the solidifying shell and the interacting distortions between deformable mold and shell materials along their interface. The ratio of the velocity of the solid/liquid interface to the mold velocity is identified as another dimensionless parameter in the analysis. In order to arrive at an analytical solution for the contact pressure along the shell/mold interface, we assume that this parameter is small. This makes the velocity ratio a convenient perturbation parameter for the analysis of thermomechanical distortion of the thin shell material as it grows from the melt. This necessarily limits the analysis to situations where the mold moves at faster rather than slower speeds. It is assumed that there is zero tangential shear stress between the fluid and the solidifying shell. As the molten liquid flows over the mold, it perfectly wets the surface. This precludes wetting effects due to surface tension. A hypoelastic constitutive law, which is a rate formulation of thermoelasticity, is assumed to govern deformation of the shell as it grows from the molten liquid. Latent heat liberated at the freezing front is extracted across a constant contact resistance at the shell/mold interface. Peculiar fluid motion at the tip is neglected. A solution for the contact pressure that is valid near the liquid surface (i.e., the meniscus) is derived from the main theoretical developments. Beyond the time of gap nucleation at the shell/mold interface, the model is no longer valid since it cannot account for gross distortion of the shell (i.e., distortions that greatly exceed the spatial perturbations considered in the model).     

Comparison of three techniques used to measure diffusive gas exchange from sheltered aquatic surfaces

Three approaches commonly used to quantify diffusive gas exchange across aquatic surfaces were compared in a densely treed, low-wind environment Diffusive surface fluxes of carbon dioxide (CO2) and methane (CH4) from a small boreal reservoir were estimated using (i) surface water concentrations, the thin boundary layer (TBL) equation, and gas transfer velocities (k) calculated using sulfur hexafluoride (SF6); (ii) surface water concentrations, the TBL equation, and k estimated from wind speed; and (iii) static floating chambers (FCs). Comparisons were made during three different approximately 10-day intervals (August 2000, June and September 2001). CO2 and CH4 fluxes estimated from SF6-derived k were on average 1-3 times greater than those determined from wind-estimated k Overall agreement between FC CO2 and CH4 flux estimates and those based on SF6 and wind speed derived kvalues was much weaker, with FC CO2 and CH4 flux estimates ranging from -9 to 23 times those based on SF6 and wind-estimated k values. Chamber deployment likely enhanced gas transfer through disturbance of the surface boundary layer, and results of this study suggest that caution must be exercised concerning the use of FCs on very still water surfaces. Furthermore, findings of this study contradict the common belief that use of wind speed to approximate k is inappropriate for small bodies of water characterized by low winds and surface obstructions.     

Power assisted fuel cell

A hybrid fuel cell demonstrated pulse power capability at pulse power load simulations synonymous with electronics and communications equipment. The hybrid consisted of a 25.0 W Proton Exchange Membrane Fuel Cell (PEMFC) stack in parallel with a two-cell lead-acid battery. Performance of the hybrid PEMFC was superior to either the battery or fuel cell stack alone at the 18.0 W load. The hybrid delivered a flat discharge voltage profile of about 4.0 V over a 5 h radio continuous transmit mode of 18.0 W.     

Social media and political partisanship – A subaltern public sphere’s role in democracy

Social media, as a subaltern public sphere (Fraser, 1990), have a democratic function in providing an alternative platform for minorities and marginalized to defy mainstream discourses in the public sphere. However, social media have been found to have an echo chamber effect, which may be detrimental to democracy. They may help to accelerate the ascendancy of a “post-truth” era in which objective facts are less influential in shaping public opinion than appeals to emotion and personal belief. A study on political polarization, however, showed that selective exposure and avoidance in social media are weak indicators of polarization (Johnson et al., 2017). This study examines the role of social media in democracy and partisan politics. The authors considered that despite the echo chamber effect, social media have a limited part to play in the formation of polarized stances compared with other factors, such as demographics, political orientation, and mass media use. The study tested two main hypotheses: H1: Social media use is associated with political stance that is marginalized in the mainstream media; H2: Political orientation has a stronger relationship than social media use with the stance toward political values and social issues.The results supported both hypotheses. Social media are associated with political stance that is marginalized in the mainstream media. However, when compared with other factors, the relationship between social media and stance becomes less obvious. Although the echo chamber effect may reinforce the original stance, social media do not exhibit a strong relationship with the stance toward political values and social issues. Partisan orientation and use of partisan mass media are found to have stronger links with variations in stance. Social media, however, provide a subaltern public sphere for those excluded from the dominant public sphere, thus extending the public sphere to accommodate multiple opinions and perspectives.     

Analysis of reactions during sintering of CuO-doped 3Y-TZP nano-powder composites

3Y-TZP (yttria-doped tetragonal zirconia) and CuO nano powders were prepared by co-precipitation and copper oxalate complexation–precipitation techniques, respectively. During sintering of powder compacts (8 mol% CuO-doped 3Y-TZP) of this two-phase system several solid-state reactions clearly influence densification behaviour. These reactions were analysed by several techniques like XPS, DSC/TGA and high-temperature XRD. A strong dissolution of CuO in the 3Y-TZP matrix occurs below 600 °C, resulting in significant enrichment of CuO in a 3Y-TZP grain-boundary layer with a thickness of several nanometres. This “transient” liquid phase strongly enhances densification. Around 860 °C a solid-state reaction between CuO and yttria as segregated to the 3Y-TZP grain boundaries occurs, forming Y₂Cu₂O₅. This solid-state reaction induces the formation of the thermodynamic stable monoclinic zirconia phase. The formation of this solid phase also retards densification. Using this knowledge of microstructural development during sintering it was possible to obtain a dense nano–nano composite with a grain size of only 120 nm after sintering at 960 °C.     

In vivo lipolysis induced by heparin injection or fasting: a proton MR spectroscopy analysis of human plasma

The impact of induced lipolysis on the composition of plasma lipids is analyzed by proton magnetic resonance spectroscopy (MRS) in humans. The variations of the methylene and methyl resonances from lipids in lipoproteins are studied under two sets of lipolytic conditions: acute endovascular lipolysis induced by an intravenous injection of heparin and subacute lipolysis induced by short fasting. During acute lipolysis, the degradation of the very low density lipoproteins structures is well correlated to the modifications observed in the areas of CH₂ and CH₃ MRS signals. The comparison of regular spectra, spectra with water signal suppression, and spectra recorded with a spin-echo sequence provides information on the behavior of the different parts of the lipoproteins, that is, the neutral core, little affected by heparine-induced lipoprotein lipase (LPL) activation, and the surface layer supplying substrates to LPL. During 48 h of fasting, only limited modifications occur on the MR spectra, and lipolysis cannot be documented in details.Address for correspondence: CRMBM-CNRS, Faculté de Médecine, 27, bd Jean Moulin, 13005 Marseille, France.