Amplification and expression of c-erbB2 oncogene in normal, hyperplastic, and malignant endometria

OBJECTIVE: To study the correlation of development and survival with amplification of c-erbB2 oncogene in endometrial carcinoma. METHODS: The amplification and expression of c-erbB2 oncogene were determined from formalin-fixed, paraffin-embedded tissues of 25 normal, 31 hyperplastic, and 72 malignant samples of the endometrium in 128 patients, using differential polymerase chain reaction (DPCR) and an immunohistochemical technique. RESULTS: Amplified c-erbB2 (2 to 12 copies) were found in two of 25 (8.0%) normal, 15 of 31 (48.4%) hyperplastic, and 45 of 72 (62.5%) malignant samples. When the results of DPCR were compared with those of the immunohistochemical method, the negative findings concide well with one another, i.e., nonamplification was associated with the absence of immunoreactivity. It was noted that the amplified c-erbB2 was found more significantly in complex and a typical hyperplasias than in simple hyperplasias. The high-level c-erbB2 amplification (at least five copies) was significantly correlated with the histological grade of endometrial carcinoma and vascular or lymphatic invasion. No correlation was seen between c-erbB2 amplification and overall survival in the present group of patients. CONCLUSIONS: The amplified c-erbB2 may play a potential role in the early development of some endometrial carcinomas. The high-level c-erbB2 amplification may identify a subset of aggressive endometrial carcinoma that involves vascular or lymphatic invasiveness and poor cell differentiation playing the role of a marker for clinical prognosis.     

Nucleoside-diphosphate kinase-mediated signal transduction via histidyl-aspartyl phosphorelay systems in Escherichia coli

Nucleoside-diphosphate kinase (NDP kinase), a key enzyme in nucleotide metabolism, is also known to be involved in growth and developmental control and tumor metastasis suppression. Interestingly, we find that coexpression of NDP kinase with Taz1, a Tar/EnvZ chimera, in the absence of its native signal, can activate a porin gene ompC-lacZ expression in Escherichia coli. Further studies show that NDP kinase can act as a protein kinase to phosphorylate histidine protein kinases such as EnvZ and CheA which are members of the His-Asp phosphorelay signal transduction systems in E. coli. Instead of ATP, the exclusive phosphodonor for histidine kinases, GTP can be utilized in vitro in the presence of NDP kinase to phosphorylate EnvZ and CheA, which then transfer the phosphoryl group to OmpR and CheY, the respective response regulators. The direct involvement of GTP for the phosphorylation of EnvZ through NDP kinase was further demonstrated by the use of a mutant EnvZ, which lost ability to be autophosphorylated with ATP. Phospho-OmpR thus formed can bind specifically to an ompF promoter sequence. These results suggest that NDP kinase may play a physiological role in signal transduction.     

基于公共存储器进行数据交换的高速数字图象处理系统

本文介绍了一种基于公共存储器进行数据交换的高速数字图象处理系统．给出了公共存储器的设计，描述了处理器对公共存储器的进程同步控制．     

Optimal Hybrid Control for Structures

A new method for integrated design of passive and active elements is presented. Rather than the existing qualitative selection of parameters for passive elements, a quantitative approach is proposed that finds optimal active and passive parameters with respect to an H₂/H_∞ performance requirement. This new approach automatically yields passive designs when the given performance limits are high enough and active (hybrid) designs when the given performance constraints are stringent. Furthermore, our algorithm finds the special performance requirement (the peak of the frequency response) that cannot be satisfied by any passive design. Hence this article shows how to determine when control is required rather than assuming a priori that it is or is not required. A simple design method given herein yields either passive, active, or hybrid designs depending only on the level of the performance constraints that are specified in the statement of the problem.     

Direct imaging of a high-power diode laser cavity using a transparent indium-tin-oxide (ITO) contact

We describe a novel "see-through" indium-tin-oxide contact on the n-side of a high power unstable resonator semiconductor laser (URSL) that allows direct observation of the cavity during high power operation. Under optimized annealing conditions this transparent ITO contact has a low enough specific contact resistivity to permit normal high power CW operation of the URSL and allows the observation of filamentation. This contact scheme is applicable to a wide range of semiconductor lasers and is especially appropriate for high power devices. The same structure can also be used to obtain a 2-D thermal map of the laser cavity.     

观赏草在庭园中的设计与应用

观赏草以其灵动的景观特质，草类特有的自然色调，飘逸的质感越来越受到设计师们的青睐，用于焦点、绿蒿、花镜、镶边均有不凡的表现，虽为草类，却有着灌木的功能。     

ρ-混合噪声中ρ-混合信号Min-Max检测的注记

本文考察了ρ-混合噪声中ρ-混合随机信号的 Min-Max 检测问题.首先,将[5]中(?)-混合条件减弱为ρ-混合相依条件;其次,去掉了[5]中式(5)(?)r_n<∞的限制条件;第三,将[5]中常值的弱信号拓广到具有ρ-混合相依的随机信号,故本文改进了[5]中结果.