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排序方式: 共有517条查询结果,搜索用时 218 毫秒
81.
Petr Ryšánek Marek Malý Pavla Čapková Martin Kormunda Zdeňka Kolská Milan Gryndler Ondřej Novák Lucie Hocelíková Lukáš Bystrianský Marcela Munzarová 《Journal of Polymer Research》2017,24(11):208
Antibacterial nylon 6 (PA6) nanofibers have been prepared in one-step procedure using Nanospider technology. Chlorhexidine (CHX), 1-dodecyltrimethylamonium bromide (DTAB) and benzyltrimethylamonium bromide (BTAB) have been used as antibacterial agents. Samples were characterized by a series of analytical and testing methods to investigate the surface chemistry, zeta potential, structure, morphology, phase composition, mechanical properties and antibacterial activity. Experimental characterization has been combined with molecular modeling to analyze the interaction of nanofibers with modifying molecules for better understanding the effect of nanofibers modification on their properties. Antibacterial modification of PA6 led to significant changes of zeta potential (from -31 mV for PA 6 up to -49 mV for PA6/BTAB), changes in phase composition (decrease of alpha phase content and increase of gamma phase content for PA6/BTAB and PA6/CHX) and to significant increase of fiber diameter for PA6/BTAB. Antibacterial modification resulted in the straightening of nanofibers and to higher permeability of nanofiber textile for all investigated samples. Tensile tests showed the the increase of Young modulus for all the investigated samples. All the modified samples: PA6/DTAB, PA6/BTAB and PA6/CHX exhibit good antibacterial activity. 相似文献
82.
Design and Synthesis of Galactosylated Bifurcated Ligands with Nanomolar Affinity for Lectin LecA from Pseudomonas aeruginosa 下载免费PDF全文
Dr. Anthony Angeli Muchen Li Dr. Lucie Dupin Dr. Gérard Vergoten Mathieu Noël Mimouna Madaoui Dr. Shuai Wang Albert Meyer Thomas Géhin Dr. Sébastien Vidal Dr. Jean‐Jacques Vasseur Dr. Yann Chevolot Dr. François Morvan 《Chembiochem : a European journal of chemical biology》2017,18(11):1036-1047
Lectin A (LecA) from Pseudomonas aeruginosa is an established virulence factor. Glycoclusters that target LecA and are able to compete with human glycoconjugates present on epithelial cells are promising candidates to treat P. aeruginosa infection. A family of 32 glycodendrimers of generation 0 and 1 based on a bifurcated bis‐galactoside motif have been designed to interact with LecA. The influences both of the central multivalent core and of the aglycon of these glycodendrimers on their affinity toward LecA have been evaluated by use of a microarray technique, both qualitatively for rapid screening of the binding properties and also quantitatively (Kd). This has led to high‐affinity LecA ligands with Kd values in the low nanomolar range (Kd=22 nm for the best one). 相似文献
83.
84.
The paper deals with a study of tensile strength and disintegration time of compacts made from silicified microcrystalline celluloses, Prosolv SMCC 90, and Prosolv HD 90, in dependence on compression force, addition of two types of lubricants, and two active ingredients. The lubricants were magnesium stearate and sodium stearyl fumarate in a concentration of 0.5%, the active ingredients being ascorbic acid and acetylsalicylic acid in a concentration of 50%.
Prosolv SMCC 90 proved to be better compatible than Prosolv HD 90; the compacts were of higher strength, which was markedly increased with increasing compression force. Prosolv HD 90 was more sensitive to additions of lubricants, and a greater decrease in strength was recorded due to the influence of sodium stearyl fumarate. The effect of lubricants on the strength of compacts in the presence of active ingredients was not identical. The disintegration time of compacts from Prosolv HD 90 without as well as with lubricants was shorter than from Prosolv SMCC 90 and was increasing with increasing compression force. Disintegration time was increased with added lubricants, and it was markedly shortened by addition of active ingredients. Compacts containing ascorbic acid possessed a shorter disintegration time than those containing acetylsalicylic acid, and it was not markedly influenced by the presence of lubricants. 相似文献
Prosolv SMCC 90 proved to be better compatible than Prosolv HD 90; the compacts were of higher strength, which was markedly increased with increasing compression force. Prosolv HD 90 was more sensitive to additions of lubricants, and a greater decrease in strength was recorded due to the influence of sodium stearyl fumarate. The effect of lubricants on the strength of compacts in the presence of active ingredients was not identical. The disintegration time of compacts from Prosolv HD 90 without as well as with lubricants was shorter than from Prosolv SMCC 90 and was increasing with increasing compression force. Disintegration time was increased with added lubricants, and it was markedly shortened by addition of active ingredients. Compacts containing ascorbic acid possessed a shorter disintegration time than those containing acetylsalicylic acid, and it was not markedly influenced by the presence of lubricants. 相似文献
85.
We prepared interpenetrating polymer networks (IPNs) composed of a gelatin hydrogel and a HydroThaneTM elastomer to combine the advantages of both polymers into one biomaterial. Fourier transform Infrared (FTIR) spectroscopy
and Differential Scanning Calorimetry (DSC) confirmed the co-existence of the two polymers in the IPNs. Optical light microscopy
confirmed hydrogel domains were interspaced into an elastomer network. Hydration and stability studies in aqueous solution
showed that, although the IPN biomaterials exhibited stable swelling for more than 30 days, approximately 10% and 50% loss
of the hydrogel component were confirmed at room temperature and 37 °C, respectively, using gel permeation chromatography
(GPC). The swelling study in the serum-containing medium indicated the biomaterials maintained their swelling stability for
different periods, depending on the extent of gelatin methacrylation, photoinitiator concentration and incubation temperature.
Lastly, the biomaterials exhibited higher failure stress and lower failure strain in a dry state than in a swollen state,
and showed limited changes in both stress and strain at room temperature and at 37 °C, in contrast with a decrease at 50 °C.
No significant effects of gelatin methacrylation on mechanical properties were noticed. The preparation and characterization
methods were well established and formed the basis of further developing the biomaterials. 相似文献
86.
Gallet C Ibanez S Zinger L Taravel FR Trierweiler M Jeacomine I Despres L 《Journal of chemical ecology》2007,33(11):2078-2089
Plant-seed parasite pollination mutualisms involve a specific pollinator whose larvae develop by consuming a fraction of the
host plant seeds. These mutualisms are stable only if the plant can control seed destruction by the larvae. Here, we studied
the chemical response of the European globeflower Trollius europaeus to infestation by an increasing number of Chiastocheta fly larvae. We used liquid chromatographic analysis to compare the content of phenolic compounds in unparasitized and parasitized
fruits collected in two natural populations of the French Alps, and mass spectrometry and nuclear magnetic resonance to elucidate
the structure of adonivernith, a C-glycosyl-flavone. This compound is present in many of the organs of T. europaeus, but not found in other Trollius species. Furthermore, it is overproduced in the carpel walls of parasitized fruits, and this induced response to infestation
by fly larvae is density-dependent (increases with larval number), and site-dependent (more pronounced in the high-altitude
site). Mechanical damage did not induce adonivernith production. This tissue-specific and density-dependent response of T. europaeus to infestation by Chiastocheta larvae might be an efficient regulation mechanism of seed-predator mutualist population growth if it decreases survival or
growth of the larvae. 相似文献
87.
Sbastien Larrch Jean-Philippe Chippaux Lucie Chevillard Simon Math Dabor Rsire Virginie Siguret Bruno Mgarbane 《International journal of molecular sciences》2021,22(17)
Toxins from Bothrops venoms targeting hemostasis are responsible for a broad range of clinical and biological syndromes including local and systemic bleeding, incoagulability, thrombotic microangiopathy and macrothrombosis. Beyond hemostais disorders, toxins are also involved in the pathogenesis of edema and in most complications such as hypovolemia, cardiovascular collapse, acute kidney injury, myonecrosis, compartmental syndrome and superinfection. These toxins can be classified as enzymatic proteins (snake venom metalloproteinases, snake venom serine proteases, phospholipases A2 and L-amino acid oxidases) and non-enzymatic proteins (desintegrins and C-type lectin proteins). Bleeding is due to a multifocal toxicity targeting vessels, platelets and coagulation factors. Vessel damage due to the degradation of basement membrane and the subsequent disruption of endothelial cell integrity under hydrostatic pressure and tangential shear stress is primarily responsible for bleeding. Hemorrhage is promoted by thrombocytopenia, platelet hypoaggregation, consumption coagulopathy and fibrin(ogen)olysis. Onset of thrombotic microangiopathy is probably due to the switch of endothelium to a prothrombotic phenotype with overexpression of tissue factor and other pro-aggregating biomarkers in association with activation of platelets and coagulation. Thrombosis involving large-caliber vessels in B. lanceolatus envenomation remains a unique entity, which exact pathophysiology remains poorly understood. 相似文献
88.
Rodent models of Parkinson’s disease are based on transgenic expression of mutant synuclein, deletion of PD genes, injections of MPTP or rotenone, or seeding of synuclein fibrils. The models show histopathologic features of PD such as Lewi bodies but mostly only subtle in vivo manifestations or systemic toxicity. The models only partly mimic a predominant loss of dopaminergic neurons in the substantia nigra. We therefore generated mice that express the transgenic diphtheria toxin receptor (DTR) specifically in DA neurons by crossing DAT-Cre mice with Rosa26 loxP-STOP-loxP DTR mice. After defining a well-tolerated DTx dose, DAT-DTR and DTR-flfl controls were subjected to non-toxic DTx treatment (5 × 100 pg/g) and subsequent histology and behavioral tests. DAT protein levels were reduced in the midbrain, and tyrosine hydroxylase-positive neurons were reduced in the substantia nigra, whereas the pan-neuronal marker NeuN was not affected. Despite the promising histologic results, there was no difference in motor function tests or open field behavior. These are tests in which double mutant Pink1−/−SNCAA53T Parkinson mice show behavioral abnormalities. Higher doses of DTx were toxic in both groups. The data suggest that DTx treatment in mice with Cre/loxP-driven DAT-DTR expression leads to partial ablation of DA-neurons but without PD-reminiscent behavioral correlates. 相似文献
89.
90.
Antonín Mikš Jiří Novák Pavel Novák 《International journal for numerical methods in engineering》2010,82(4):525-536
The quadrature of general, highly oscillatory integrals is a relatively complicated computational problem that occurs in a wide range of practical applications, e.g. in physics, chemistry, and image analysis. It is often necessary to use a high number of nodal points with classical quadrature formulas in order to achieve a required accuracy of numerical integration of rapidly oscillating functions. However, an increase in integration points leads to a larger computational time. Our work describes and analyses a method for numerical integration of rapidly oscillating functions, which enables to obtain the required accuracy with a smaller number of nodal points than classical integration rules and with a relatively simple integration formula. The proposed method is generally suitable for integration of rapidly oscillating functions in various applications. The method is demonstrated in examples of calculation of the diffraction integral in optics, where the integrand is often a rapidly oscillatory function. The results can be readily adapted to similar problems from other fields. Copyright © 2009 John Wiley & Sons, Ltd. 相似文献