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721.
722.
The physicochemical properties and electrochemical behavior of platinized titanium anodes produced by a combined electrochemical method, which involves electrodeposition of a thin platinum layer on a titanium substrate followed by heat treatment, are studied. The process is shown to result in the appearance of a composite coating with a substantial content of titanium oxides. The composite obtained is an n-type semiconductor, the flat-band potentials of which and the number of charge carriers depend on the preparation conditions. It is proposed to use the reduction peak of oxygen-containing platinum compounds on the stripping voltammogram as a correlation parameter for predicting the electrocatalytic activity of heat-treated Ti/Pt composite electrodes with respect to the oxygen evolution reaction.  相似文献   
723.
    
Effects of isotopic substitution on the rate constants of human dihydrofolate reductase (HsDHFR), an important target for anti-cancer drugs, have not previously been characterized due to its complex fast kinetics. Here, we report the results of cryo-measurements of the kinetics of the HsDHFR catalyzed reaction and the effects of protein motion on catalysis. Isotopic enzyme labeling revealed an enzyme KIE (kHLE/kHHE) close to unity above 0 °C; however, the enzyme KIE was increased to 1.72±0.15 at −20 °C, indicating that the coupling of protein motions to the chemical step is minimized under optimal conditions but enhanced at non-physiological temperatures. The presented cryogenic approach provides an opportunity to probe the kinetics of mammalian DHFRs, thereby laying the foundation for characterizing their transition state structure.  相似文献   
724.
    
Attribution of specific roles to the two ubiquitously expressed PI 3-kinase (PI3K) isoforms p110α and p110β in biological functions they have been implicated, such as in insulin signalling, has been challenging. While p110α has been demonstrated to be the principal isoform activated downstream of the insulin receptor, several studies have provided evidence for a role of p110β. Here we have used isoform-selective inhibitors to estimate the relative contribution of each of these isoforms in insulin signalling in adipocytes, which are a cell type with essential roles in regulation of metabolism at the systemic level. Consistent with previous genetic and pharmacological studies, we found that p110α is the principal isoform activated downstream of the insulin receptor under physiological conditions. p110α interaction with Ras enhanced the strength of p110α activation by insulin. However, this interaction did not account for the selectivity for p110α over p110β in insulin signalling. We also demonstrate that p110α is the principal isoform activated downstream of the β-adrenergic receptor (β-AR), another important signalling pathway in metabolic regulation, through a mechanism involving activation of the cAMP effector molecule EPAC1. This study offers further insights in the role of PI3K isoforms in the regulation of energy metabolism with implications for the therapeutic application of selective inhibitors of these isoforms.  相似文献   
725.
现有的BIM应用软件提供的乔木模型多为RPC或高精度3D模型等3D CAD类型。这些模型着重于表达美感或进行展示,无法反映根系情况。虽然可能包含一定附加属性,但表达乔木生长和空间需求的功能有限。因而,冲突检查、工序调度等BIM的优势功能无从应用。风景园林实践领域已就此问题开始构建自己的模型库,但能够准确反映现状树木真实尺寸体量,以及现状树木和规划树木未来空间需求的模型仍未广泛出现。提出一种基于树冠、树干和根系构型与生长功能的实体/网面封装建模方法构建BIM乔木模型。模型形态通过一组参数调整,并根据树龄和反映环境限制因子的值来反映其变化。所包含的数据和参数数量随后期设计阶段所需的LoD(详细层级)及专业实践的具体要求而增加。该模型可以与提供本地区内外苗木商品信息的植物数据库连接使用。  相似文献   
726.
When computing linear prediction (LP) parameters of speech, large numbers of data are uninformative in a certain set-theoretic sense, and the expense of updating the estimates at these times can be avoided. “Set-membership” (SM) identification is formulated as a weighted recursive covariance LP problem with a special criterion for dynamic weight determination. An algorithm is developed which can be implemented on a systolic processor if desired, but which retains a simple interpretation as a specially weighted convariance LP method. The algorithm is applied to identification of the LP parameters of real speech data, and a number of practical issues are discussed. The potential for an adaptive strategy and other open research questions generated by the experimental work are discussed in the conclusions.  相似文献   
727.
The processing conditions in an air suspension column were found to be critical in producing controlled release polyethylene glycol-silicone elastomer tablet film coatings. The coating equipment used was also shown to play a major role in determining the permeability of the resultant tablet coatings. This pronounced process dependent phenomenon was explained by the film layering mechanism involved in the formation of the polyethylene glycol containing silicone elastomer coating on tablets.  相似文献   
728.
The silicone elastomer latex containing colloidal silica and poloyoxyethylene glycol 8,000 was shown to produce controlled released film coating on potassium chloride tablets with different shapes. The tablet shape did not affect the zero-order release characteristic of the active ingredinent from the coated tablests. With the same coating weight, the capsule shaped tablets exhibited a faster drug release rate as compared to the oval and round deep-cup shaped tablets.  相似文献   
729.
730.
A correlation is established experimentally between the real “faceted” structure of the cutting area of a blade according to electron microscopy data and the spatial quantization of an image of the recorded speckle field (upon the maximum compression of the recorded information to logical zeroes and ones) in coherent optical analyses of the diffraction effect at the blade edge. This correlation may prove useful for solving the inverse technical problem (with equally justified variants of concepts for the theoretical modeling of a specific situation). Pis’ma Zh. Tekh. Fiz. 23, 42–46 (October 12, 1997)  相似文献   
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