智慧BIM乔木模型：从设计图纸到施工 现场

现有的BIM应用软件提供的乔木模型多为RPC或高精度3D模型等3D CAD类型。这些模型着重于表达美感或进行展示,无法反映根系情况。虽然可能包含一定附加属性,但表达乔木生长和空间需求的功能有限。因而,冲突检查、工序调度等BIM的优势功能无从应用。风景园林实践领域已就此问题开始构建自己的模型库,但能够准确反映现状树木真实尺寸体量,以及现状树木和规划树木未来空间需求的模型仍未广泛出现。提出一种基于树冠、树干和根系构型与生长功能的实体/网面封装建模方法构建BIM乔木模型。模型形态通过一组参数调整,并根据树龄和反映环境限制因子的值来反映其变化。所包含的数据和参数数量随后期设计阶段所需的LoD(详细层级)及专业实践的具体要求而增加。该模型可以与提供本地区内外苗木商品信息的植物数据库连接使用。     

D-Peptide and D-Protein Technology: Recent Advances,Challenges, and Opportunities**

Total chemical protein synthesis provides access to entire D-protein enantiomers enabling unique applications in molecular biology, structural biology, and bioactive compound discovery. Key enzymes involved in the central dogma of molecular biology have been prepared in their D-enantiomeric forms facilitating the development of mirror-image life. Crystallization of a racemic mixture of L- and D-protein enantiomers provides access to high-resolution X-ray structures of polypeptides. Additionally, D-enantiomers of protein drug targets can be used in mirror-image phage display allowing discovery of non-proteolytic D-peptide ligands as lead candidates. This review discusses the unique applications of D-proteins including the synthetic challenges and opportunities.     

Magnetic Polaron States in Photoluminescent Carbon Dots Enable Hydrogen Peroxide Photoproduction (Small 32/2023)

Photoactivation of aspartic acid-based carbon dots (Asp-CDs) induces the generation of spin-separated species, including electron/hole (e⁻/h⁺) polarons and spin-coupled triplet states, as uniquely confirmed by the light-induced electron paramagnetic resonance spectroscopy. The relative population of the e⁻/h⁺ pairs and triplet species depends on the solvent polarity, featuring a substantial stabilization of the triplet state in a non-polar environment (benzene). The electronic properties of the photoexcited Asp-CDs emerge from their spatial organization being interpreted as multi-layer assemblies containing a hydrophobic carbonaceous core and a hydrophilic oxygen and nitrogen functionalized surface. The system properties are dissected theoretically by density functional theory in combination with molecular dynamics simulations on quasi-spherical assemblies of size-variant flakelike model systems, revealing the importance of size dependence and interlayer effects. The formation of the spin-separated states in Asp-CDs enables the photoproduction of hydrogen peroxide (H₂O₂) from water and water/2-propanol mixture via a water oxidation reaction.     

Spatio-Temporal Photoactivation of Cytotoxic Proteins

Protein therapeutics offer exquisite selectivity in targeting cellular processes and behaviors, but are rarely used against non-cell surface targets due to their poor cellular uptake. While cell-penetrating peptides can be used to deliver recombinant proteins to the cytosol, it is generally difficult to selectively deliver active proteins to target cells. Here, we report a recombinantly produced, intracellular protein delivery and targeting platform that uses a photocaged intein to regulate the spatio-temporal activation of protein activity in selected cells upon irradiation with light. The platform was successfully demonstrated for two cytotoxic proteins to selectively kill cancer cells after photoactivation of intein splicing. This platform can generically be applied to any protein whose activity can be disrupted by a fused intein, allowing it to underpin a wide variety of future protein therapeutics.