全文获取类型
收费全文 | 175篇 |
免费 | 11篇 |
专业分类
电工技术 | 2篇 |
综合类 | 1篇 |
化学工业 | 68篇 |
金属工艺 | 12篇 |
机械仪表 | 1篇 |
建筑科学 | 6篇 |
矿业工程 | 1篇 |
能源动力 | 3篇 |
轻工业 | 3篇 |
无线电 | 12篇 |
一般工业技术 | 31篇 |
冶金工业 | 7篇 |
原子能技术 | 2篇 |
自动化技术 | 37篇 |
出版年
2024年 | 1篇 |
2023年 | 1篇 |
2022年 | 8篇 |
2021年 | 20篇 |
2020年 | 3篇 |
2019年 | 6篇 |
2018年 | 6篇 |
2017年 | 7篇 |
2016年 | 12篇 |
2015年 | 8篇 |
2014年 | 14篇 |
2013年 | 11篇 |
2012年 | 13篇 |
2011年 | 21篇 |
2010年 | 10篇 |
2009年 | 9篇 |
2008年 | 5篇 |
2007年 | 10篇 |
2006年 | 7篇 |
2005年 | 4篇 |
2003年 | 2篇 |
2002年 | 2篇 |
2001年 | 1篇 |
1998年 | 1篇 |
1997年 | 1篇 |
1994年 | 2篇 |
1993年 | 1篇 |
排序方式: 共有186条查询结果,搜索用时 171 毫秒
181.
We report on the generation of nanometer-wide, non-topographical patterns of proteins on planar surfaces. In particular, we used the regular lattice of reconstituted microtubules as template structures to specifically bind and transfer kinesin-1 and nonclaret disjunctional motor proteins. The generated tracks, which comprise dense and structurally oriented arrays of functional motor proteins, proved to be highly efficient for the guiding of microtubule transporters. 相似文献
182.
Joanna Filarowska-Jurko Lukasz Komsta Irena Smaga Paulina Surowka Marta Marszalek-Grabska Pawel Grochecki Dorota Nizio Malgorzata Filip Jolanta H. Kotlinska 《International journal of molecular sciences》2022,23(10)
Adverse early life experiences are associated with an enhanced risk for mental and physical health problems, including substance abuse. Despite clinical evidence, the mechanisms underlying these relationships are not fully understood. Maternal separation (MS) is a commonly used animal model of early neglect. The aim of the current study is to determine whether the N-methyl-D-aspartate receptor (NMDAR)/glycine sites are involved in vulnerability to alcohol consumption (two-bottle choice paradigm) and reversal learning deficits (Barnes maze task) in adolescent rats subjected to the MS procedure and whether these effects are sex dependent. By using ELISA, we evaluated MS-induced changes in the NMDAR subunits (GluN1, GluN2A, GluN2B) expression, especially in the glycine-binding subunit, GluN1, in the prefrontal cortex (PFC) and ventral striatum (vSTR) of male/female rats. Next, we investigated whether Org 24598, a glycine transporter 1 (GlyT1) inhibitor, was able to modify ethanol drinking in adolescent and adult male/female rats with prior MS experience and reversal learning in the Barnes maze task. Our findings revealed that adolescent MS female rats consumed more alcohol which may be associated with a substantial increase in GluN1 subunit of NMDAR in the PFC and vSTR. Org 24598 decreased ethanol intake in both sexes with a more pronounced decrease in ethanol consumption in adolescent female rats. Furthermore, MS showed deficits in reversal learning in both sexes. Org 24598 ameliorated reversal learning deficits, and this effect was reversed by the NMDAR/glycine site inhibitor, L-701,324. Collectively, our results suggest that NMDAR/glycine sites might be targeted in the treatment of alcohol abuse in adolescents with early MS, especially females. 相似文献
183.
Takenobu Ishii Montserrat Ruiz-Torruella Kenta Yamamoto Tsuguno Yamaguchi Alireza Heidari Roodelyne Pierrelus Elizabeth Leon Satoru Shindo Mohamad Rawas-Qalaji Maria Rita Pastore Atsushi Ikeda Shin Nakamura Hani Mawardi Umadevi Kandalam Patrick Hardigan Lukasz Witek Paulo G. Coelho Toshihisa Kawai 《International journal of molecular sciences》2022,23(10)
It is well known that Semaphorin 4D (Sema4D) inhibits IGF-1-mediated osteogenesis by binding with PlexinB1 expressed on osteoblasts. However, its elevated level in the gingival crevice fluid of periodontitis patients and the broader scope of its activities in the context of potential upregulation of osteoclast-mediated periodontal bone-resorption suggest the need for further investigation of this multifaceted molecule. In short, the pathophysiological role of Sema4D in periodontitis requires further study. Accordingly, attachment of the ligature to the maxillary molar of mice for 7 days induced alveolar bone-resorption accompanied by locally elevated, soluble Sema4D (sSema4D), TNF-α and RANKL. Removal of the ligature induced spontaneous bone regeneration during the following 14 days, which was significantly promoted by anti-Sema4D-mAb administration. Anti-Sema4D-mAb was also suppressed in vitro osteoclastogenesis and pit formation by RANKL-stimulated BMMCs. While anti-Sema4D-mAb downmodulated the bone-resorption induced in mouse periodontitis, it neither affected local production of TNF-α and RANKL nor systemic skeletal bone remodeling. RANKL-induced osteoclastogenesis and resorptive activity were also suppressed by blocking of CD72, but not Plexin B2, suggesting that sSema4D released by osteoclasts promotes osteoclastogenesis via ligation to CD72 receptor. Overall, our data indicated that ssSema4D released by osteoclasts may play a dual function by decreasing bone formation, while upregulating bone-resorption. 相似文献
184.
Marcin Wierzchowski Daniel Ziental Dawid aewski Artur Korzanski Agnieszka Gielara-Korzanska Ewa Tykarska Jolanta Dlugaszewska Lukasz Sobotta 《International journal of molecular sciences》2022,23(11)
Newly developed tetra- and octasubstituted methimazole-phthalocyanine conjugates as potential photosensitizers have been obtained. Synthesized intermediates and final products were characterized by the MALD-TOF technique and various NMR techniques, including 2D methods. Single-crystal X-ray diffraction was used to determine the crystal structures of dinitriles. The studied phthalocyanines revealed two typical absorption bands—the Soret band and the Q band. The most intense fluorescence was observed for octasubstituted magnesium(II) phthalocyanine in DMF (ΦFL = 0.022). The best singlet oxygen generators were octasubstituted magnesium(II) and zinc(II) phthalocyanines (Φ∆ 0.56 and 0.81, respectively). The studied compounds presented quantum yields of photodegradation at the level between 10−5 and 10−6. Due to their low solubility in a water environment, the liposomal formulations were prepared. Within the studied group, octasubstituted zinc(II) phthalocyanine at the concentration of 100 µM activated with red light showed the highest antibacterial activity against S. aureus equal to a 5.68 log reduction of bacterial growth. 相似文献
185.
186.