Proliferating cell nuclear antigen (PCNA) immunostaining--a prognostic factor in ovarian cancer?

The measurement of tumour cell proliferation is becoming increasingly recognised in defining prognostic groups. Proliferating cell nuclear antigen (PCNA) immunolocalisation can be used as an index of cell proliferation and may define the extent of departure from normal growth control. The monoclonal antibody PC10 stains PCNA in archival paraffin-embedded tissue. This study investigates its potential as a prognostic marker in early and advanced ovarian cancer. A three-stage immunoperoxidase technique was developed to detect the monoclonal antibody PC10. Archival paraffin-embedded tissue from 19 stage I ovarian tumours (13 malignant and six borderline) and 79 advanced (stage IIb-IV) ovarian tumours (patients entered into the Third North-West Thames Ovarian Cancer Trial) was immunostained with PC10. PC10 immunostaining was performed successfully in 91.8% of cases. The PC10 labelling index (PC10 LI) ranged from 1.5% to 88% with a mean value of 47.4%. Stage I borderline tumours had significantly lower PCNA labelling indexes than stage I malignant tumours (P < 0.048). In advanced disease there was an inverse correlation between PC10 and overall survival, and in those patients who underwent good debulking surgery (37 patients with disease < 2 cm diameter) a low PC10 value (< 36.5%) correlated with improved survival (log-rank trend test for survival, chi 2 = 5.75, P = 0.017). PCNA immunostaining defines a good prognostic subgroup in adequately debulked patients with ovarian cancer.     

The C-terminal domains of gammaS-crystallin pair about a distorted twofold axis

The 2-domain gammaS-crystallin, a highly conserved early evolutionaryoff-shoot of the gamma-crystallin family, is located in the water-richregion of eye lenses. The expressed C-terminal domain, gammaS-C, has beencrystallized and the 2.56 A X-ray structure determined. There are twodomains in the asymmetric unit which pair about a distorted twofold axis.One of the domains has an altered conformation in a highly conserved regionof the protein, the tyrosine corner. The distorted gammaS-C dimer ofdomains is compared with the highly symmetrical, equivalent recombinantdimer of C-terminal domains from gammaB- crystallin. Sequence changes closeto the interface, that distinguish gammaS from the other gamma-crystallins,are examined in order to evaluate their role in symmetrical domain pairing.     

一种基于S3C241OA的液晶屏接口设计

详细介绍了一种基于ARM9 S3C2410A控制的液晶显示屏的设计.在介绍了KS0107控制器的原理与使用后,给出了S3C2410A与工KS0107的硬件电路连接方法,搭建了一种以两者为基础的嵌入式液晶显示屏系统工作平台,为各种便携式系统显示前端的设计提供了一种可以借鉴的方法.     

New tools for the study of oceanic eddies: Satellite derived inherent optical properties

Satellite study of oceanic eddy formation, propagation, interactions, and fate was first conducted by sea surface temperature derived at infrared wavelengths. For visible wavelength ocean color reflectances, it is shown that recent radiative transfer model inversions provide additional characteristics of eddies: their constituent absorption and backscattering inherent optical properties. The chromophoric dissolved organic matter absorption coefficient has the highest contrast and is therefore the most visually evident inherent optical property (while the phytoplankton absorption coefficient and backscattering coefficients are respectively less discernible). For use as an analytical tool, comparisons suggests that the chromophoric dissolved organic matter absorption coefficient has a ∼10× higher contrast (i.e., ∼5% vs. 50%) in the Middle Atlantic Bight making eddy events detectable over longer time periods than with SST imagery. Example imagery illustrates the application of chromophoric dissolved organic matter and phytoplankton absorption coefficient inherent optical properties to the visual injection of dissolved and particulate organic carbon into the deep ocean by a Gulf Stream ring.     

Ink‐jet printing: The route to production of full‐color P‐OLED displays

Abstract— Ink‐jet‐printing technology for displays has been making rapid progress and is now nearing production. This paper will go through the developments that have been carried out at CDT, Ltd., to achieve this status. The ink‐jet head and machine development that underpin the capability of the RGB printing process to manufacture P‐OLEDs at high speed with high reliability and with the film‐forming properties to achieve the appropriate device performance will be described. The important contribution made by the availability of flexible metrological tools will be discussed.     

Human brain metabolic response to caffeine and the effects of tolerance

OBJECTIVE: Since there is limited information concerning caffeine's metabolic effects on the human brain, the authors applied a rapid proton echo-planar spectroscopic imaging technique to dynamically measure regional brain metabolic responses to caffeine ingestion. They specifically measured changes in brain lactate due to the combined effects of caffeine's stimulation of glycolysis and reduction of cerebral blood flow. METHOD: Nine heavy caffeine users and nine caffeine-intolerant individuals, who had previously discontinued or substantially curtailed use of caffeinated products because of associated anxiety and discomforting physiological arousal, were studied at baseline and then during 1 hour following ingestion of caffeine citrate (10 mg/kg). To assess state-trait contributions and the effects of caffeine tolerance, five of the caffeine users were restudied after a 1- to 2-month caffeine holiday. RESULTS: The caffeine-intolerant individuals, but not the regular caffeine users, experienced substantial psychological and physiological distress in response to caffeine ingestion. Significant increases in global and regionally specific brain lactate were observed only among the caffeine-intolerant subjects. Reexposure of the regular caffeine users to caffeine after a caffeine holiday resulted in little or no adverse clinical reaction but significant rises in brain lactate which were of a magnitude similar to that observed for the caffeine-intolerant group. CONCLUSIONS: These results provide direct evidence for the loss of caffeine tolerance in the human brain subsequent to caffeine discontinuation and suggest mechanisms for the phenomenon of caffeine intolerance other than its metabolic effects on elevating brain lactate.     

Evidence for terror management theory II: The effects of mortality salience on reactions to those who threaten or bolster the cultural worldview.

Three experiments were conducted to test the hypothesis, derived from terror management theory, that reminding people of their mortality increases attraction to those who consensually validate their beliefs and decreases attraction to those who threaten their beliefs. In Study 1, subjects with a Christian religious background were asked to form impressions of Christian and Jewish target persons. Before doing so, mortality was made salient to half of the subjects. In support of predictions, mortality salience led to more positive evaluations of the in-group member (the Christian) and more negative evaluations of the out-group member (the Jew). In Study 2, mortality salience led to especially negative evaluations of an attitudinally dissimilar other, but only among subjects high in authoritarianism. In Study 3, mortality salience led to especially positive reactions to someone who directly praised subjects' cultural worldviews and especially negative reactions to someone who criticized them. The implications of these findings for understanding in-group favoritism, prejudice, and intolerance of deviance are discussed. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Microbial Flora Associated with Louisiana Processed Frozen and Fresh Nutria (Myocastor Coypus) Carcasses

ABSTRACT: Nutria ( Myocastor coypus ) are semiaquatic vegetarians that have caused extensive destruction to coastal wetlands of Louisiana. There have been efforts to harvest nutria and market their meat for human consumption. This study seeks to address the microbial safety of nutria meat. Four regions of processed nutria carcasses, including the hindquarters, neck, back, and abdomen, were evaluated for microbial contamination during processing. Bacterial isolates were isolated and identified from each region of the carcass using selective and nonselective media, phenotypic tests, and the Vitek^TM identification system. The majority of microorganisms identified were spoilage organisms. The pathogenic microorganisms isolated Escherichia coli, Stophylococcus aureus , and Burkholderia mallei. This study will be used to develop an HACCP plan for processing nutria.