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131.
Y. KONDO C. SAKAE M. KUBOTA T. NAGASUE S. SATO 《Fatigue & Fracture of Engineering Materials & Structures》2004,27(5):361-368
This paper proposes a local stress concept to evaluate the fretting fatigue limit for contact edge cracks. A unique S–N curve based on the local stress could be obtained for a contact edge crack irrespective of mechanical factors such as contact pressure, relative slip, contact length, specimen size and loading type. The analytical background for the local stress concept was studied using FEM analysis. It was shown that the local stress uniquely determined the ΔK change due to crack growth as well as the stress distribution near the contact edge. The condition that determined the fretting fatigue limit was predicted by combining the ΔK change due to crack growth and the ΔKth for a short crack. The formation of a non‐propagating crack at the fatigue limit was predicted by the model and it was experimentally confirmed by a long‐life fretting fatigue test. 相似文献
132.
水轮机转轮泥沙磨损的数值模拟 总被引:1,自引:0,他引:1
本文利用κ-ε-Ap两组湍流模型计算水轮机转轮中泥沙固液两相湍流。根据液相和固相三维流速场的计算结果数值模拟了转轮叶片面的泥沙磨损。 相似文献
133.
现代建筑发展潮流中,日本建筑的设计风格独树一帜,新生代建筑师层出不穷,这离不开其独特的建筑文化环境,比如师承体系,与结构师之间的紧密合作等.此次访谈以建筑师与结构师的合作为主题,采访了石上纯也和佐藤淳两位先生,以了解和学习他们的工作模式.本文包括三部分:第一部分探讨合作模式,通过初期项目的讨论,阐述了双方合作开始的契机与发展,而海外项目中与当地事务所合作经验的对比,更显示出他们合作模式的独特性;第二部分探讨设计理念,通过两位老师在以往展览和建筑设计项目中关于自然的讨论,对场地的回应等,表达出他们彼此对建筑和结构设计理念的理解与思考;第三部分探讨对未来教育模式、新材料、新技术的建议与展望. 相似文献
134.
MIZUNA YANO KOTA HIROI TETSUYA YUASA KENJI INOUE OSAMU YAMAMOTO TAKAO NAKAMURA DAISUKE SATO ZHONGGANG FENG 《Biocell》2023,47(5):1095-1106
Background: Human heart changes its energetic substrates from lactate and glucose to fatty acids during the neonatal period. Noticing the lack of fatty acids in media for the culture of cardiomyocytes derived from human pluripotent stem cells (hiPS-CM), researchers have supplemented mixtures of fatty acids to hiPS-CM and reported the enhancement in the maturation of hiPS-CM. In our previous studies, we separately supplemented two polyunsaturated fatty acids (PUFAs), docosahexaenoic acid (DHA) or arachidonic acid (AA), to rat fetal cardiomyocytes and found that the supplementations upregulated the expressions of mRNAs for cardiomyocyte differentiation, fatty acid metabolism, and cellular adhesion. The enhancement in cellular contractility was attributed to the improvement in intercellular connection rather than a direct enhancement of the contractile force. Methods: This study reports the successive results of the effects of DHA or AA supplementation on hiPS-CM. In addition to the contractile force and mRNA measurements used in the previous study, we further investigated the effect of different cellular aggregations on the contractile force output by means of finite element analysis, measured glucose and fatty acids metabolites, and assessed cTNT and MLC2v expressions through immunofluorecsence evaluation. Results: It showed that the sole supplementation of albumin-conjugated DHA or AA can be taken up by hiPS-CM without other uptake-enhancing factors, and the supplementations may activate the CD36_ERRγ metabolic pathway. DHA or AA supplementation increased the cellular contractile ratio on collagen gels and AA supplementation stimulated hiPS-CM aggregation to form cellular clusters. The enhancement effect on the hiPS-CM contractile force was modest since the increase in contractile force was not significant. AA supplementation was more effective than DHA supplementation because it significantly upregulated mRNA expressions of P300 and CD36. However, finite element analysis showed that the formation of clusters on a collagen gel attenuated the contractile force exerted by the gel on its surroundings. Conclusion: DHA and AA, as having been supplemented in infant formulas, have no direct and significant enhancement effect on the performance of the hiPS-CM when they were supplemented individually, although they were able to enter the cellular metabolic system. The AA supplementation showed some auxiliary effect on the maturation of hiPS-CM, which is worthy of further investigation under the consideration of membrane composition alteration and remodeling of membrane molecules. 相似文献