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51.
Andr Hofmann Malte Wallmeier Marek Hauptmann Jens‐Peter Majschak 《Packaging Technology and Science》2019,32(6):287-296
In this paper, deep‐drawn paperboard cups are characterized in terms of their elongation along the cup wall. For this purpose, a method for the non‐destructive determination of the elongation was developed and tested. Using the presented method, a parameter study of the process parameters blank holder force, forming temperature, material moisture content, drawing velocity, and clearance ratio was performed. The influence of the process parameters on the cup wall elongation was determined by statistical design of experiments. Using the determined relationships between the process parameters, it was possible to achieve a shape‐specific characterization of the elongation along the cup wall. The parameter study showed a significant influence of the blank holder force, the forming temperature, and the material moisture content to the elongation along the cup wall. 相似文献
52.
Caroline Murawski Andreas Mischok Jonathan Booth Jothi Dinesh Kumar Emily Archer Laura Tropf Chang‐Min Keum Ya‐Li Deng Kou Yoshida Ifor D. W. Samuel Marcel Schubert Stefan R. Pulver Malte C. Gather 《Advanced materials (Deerfield Beach, Fla.)》2019,31(42)
Fluorescence imaging is an indispensable tool in biology, with applications ranging from single‐cell to whole‐animal studies and with live mapping of neuronal activity currently receiving particular attention. To enable fluorescence imaging at cellular scale in freely moving animals, miniaturized microscopes and lensless imagers are developed that can be implanted in a minimally invasive fashion; but the rigidity, size, and potential toxicity of the involved light sources remain a challenge. Here, narrowband organic light‐emitting diodes (OLEDs) are developed and used for fluorescence imaging of live cells and for mapping of neuronal activity in Drosophila melanogaster via genetically encoded Ca2+ indicators. In order to avoid spectral overlap with fluorescence from the sample, distributed Bragg reflectors are integrated onto the OLEDs to block their long‐wavelength emission tail, which enables an image contrast comparable to conventional, much bulkier mercury light sources. As OLEDs can be fabricated on mechanically flexible substrates and structured into arrays of cell‐sized pixels, this work opens a new pathway for the development of implantable light sources that enable functional imaging and sensing in freely moving animals. 相似文献
53.
Microbial community structure in activated sludge floc analysed by fluorescence in situ hybridization and its relation to floc stability 总被引:4,自引:0,他引:4
The efficiency of activated sludge treatment plants is dependent on the solid-liquid separation properties of the activated sludge. A critical parameter is the stability of the microbial flocs. Weak flocs deflocculate easily leaving increased concentrations of suspended solids in the effluent. The knowledge about how different bacteria are attached to the flocs and their influence on the bioflocculation is limited. In this study, the deflocculation of different phylogenetic groups of bacteria in activated sludge from a full scale plant was investigated. The experiments were carried out by using a shear method where the sludge flocs are deflocculated under controlled shear conditions. The degree of deflocculation was measured as increase in turbidity of the supernatant. Identification and quantification of the microbial community structure of both total activated sludge and deflocculated bacteria were conducted with group-specific gene probes for broad groups of bacteria (Alphaproteobacteria, Betaproteobacteria, Gammaproteobacteria and Actinobacteria) and fluorescence in situ hybridization (FISH). The microbial community structure of the deflocculated bacteria was different compared to the total activated sludge with a higher abundance of Gammaproteobacteria in the supernatant indicating that different groups of bacteria are bound with different strength to the floc surface. The results show that the bacteria in the outer parts of the flocs are relatively loosely bound to the floc matrix and can be easily eroded from the surface when exposed to shear. 相似文献
54.
55.
Philipp B Hoff M Germa F Schink B Beimborn D Mersch-Sundermann V 《Environmental science & technology》2007,41(4):1390-1398
Prediction of the biodegradability of organic compounds is an ecologically desirable and economically feasible tool for estimating the environmental fate of chemicals. We combined quantitative structure-activity relationships (QSAR) with the systematic collection of biochemical knowledge to establish rules for the prediction of aerobic biodegradation of N-heterocycles. Validated biodegradation data of 194 N-heterocyclic compounds were analyzed using the MULTICASE-method which delivered two QSAR models based on 17 activating (OSAR 1) and on 16 inactivating molecular fragments (GSAR 2), which were statistically significantly linked to efficient or poor biodegradability, respectively. The percentages of correct classifications were over 99% for both models, and cross-validation resulted in 67.9% (GSAR 1) and 70.4% (OSAR 2) correct predictions. Biochemical interpretation of the activating and inactivating characteristics of the molecular fragments delivered plausible mechanistic interpretations and enabled us to establish the following biodegradation rules: (1) Target sites for amidohydrolases and for cytochrome P450 monooxygenases enhance biodegradation of nonaromatic N-heterocycles. (2) Target sites for molybdenum hydroxylases enhance biodegradation of aromatic N-heterocycles. (3) Target sites for hydratation by an urocanase-like mechanism enhance biodegradation of imidazoles. Our complementary approach represents a feasible strategy for generating concrete rules for the prediction of biodegradability of organic compounds. 相似文献
56.
Dr. Marta Robotta Hanne R. Gerding Antonia Vogel Prof. Dr. Karin Hauser Dr. Stefan Schildknecht Dr. Christiaan Karreman Dr. Marcel Leist Prof. Dr. Vinod Subramaniam Dr. Malte Drescher 《Chembiochem : a European journal of chemical biology》2014,15(17):2499-2502
The human alpha‐Synuclein (αS) protein is of significant interest because of its association with Parkinson's disease and related neurodegenerative disorders. The intrinsically disordered protein (140 amino acids) is characterized by the absence of a well‐defined structure in solution. It displays remarkable conformational flexibility upon macromolecular interactions, and can associate with mitochondrial membranes. Site‐directed spin‐labeling in combination with electron paramagnetic resonance spectroscopy enabled us to study the local binding properties of αS on artificial membranes (mimicking the inner and outer mitochondrial membranes), and to evaluate the importance of cardiolipin in this interaction. With pulsed, twofrequency, double‐electron electron paramagnetic resonance (DEER) approaches, we examined, to the best of our knowledge for the first time, the conformation of αS bound to isolated mitochondria. 相似文献
57.
Christina Polasky Armin Steffen Kristin Loyal Christian Lange Karl-Ludwig Bruchhage Ralph Pries 《International journal of molecular sciences》2021,22(21)
Obstructive sleep apnea (OSA) is characterized by nocturnal breathing intermissions resulting in oxidative stress and eventually, a low-grade systemic inflammation. The study aimed to investigate the impact of positive airway pressure (PAP) therapy on the inflammatory milieu as measured by monocyte and T cell phenotypic alterations. Participants were assessed for their OSA severity before PAP therapy and about six months later, including patient-reported outcome and therapy usage by telemetry readout. The distributions of the CD14/CD16-characterized monocyte subsets as well as the CD4/CD8-characterized effector T cell subsets with regard to their PD-1 and PD-L1 expression were analyzed by flow cytometry from blood samples. Data of 25 patients revealed a significant reconstitution of the monocyte subset distribution and a decrease in PD-L1 expression on pan-monocytes and CD8+ T cells without an association to initial AHI and overweight. The PD-1 expression was still increased on T cell subsets, especially on CD4+ TH17/22 cells. We conclude that PAP therapy might have a rapid effect on the monocyte phenotype and overall PD-L1 expression levels. However, T cell immune alterations especially on TH17/22 cells persist longer, indicating an ongoing disturbance of the adaptive immune system. 相似文献
58.
International existieren verschiedene genormte Raumbrandprüfungen, in welchen das Brandverhalten von Wand‐ und Deckenprodukten unter realitätsnahen Einbaubedingungen untersucht wird. Die Prüfmethoden unterscheiden sich zum Teil erheblich hinsichtlich der Raumgeometrie, der Ventilationsöffnungen bzw. ‐verhältnisse sowie der Art und Leistung der verwendeten Zündquellen. Auf der Basis von Brandsimulationsrechnungen mit dem CFD‐Code FDS wurde die Intensität der resultierenden thermischen Produktbeanspruchung für ausgewählte Prüfräume vergleichend analysiert. Die Simulationsergebnisse zeigen, dass zwischen den betrachteten Methoden deutliche Unterschiede bestehen, die zu inkonsistenten Produktbeurteilungen führen können. Comparative analysis of thermal exposure conditions in standard room fire tests. Various standards exist worldwide that describe room fire tests for determining the reaction to fire behaviour of wall and ceiling products under end‐use conditions. The test methods differ with respect to the room geometry, the ventilation conditions and the type and heat output of the ignition source. Numerical simulations were performed using the CFD code FDS to comparatively analyse the resulting thermal exposure conditions for selected test rooms. The simulation results exhibit considerable differences between the analysed test methods, which may lead to inconsistent product assessments. 相似文献
59.
Solar power technologies will have to become a major pillar in the world's future energy system to combat climate change and resource depletion. However, it is unclear which solar technology is and will prove most viable. Therefore, a comprehensive comparative assessment of solar technologies along the key quantitative and qualitative competitiveness criteria is needed. Based on a literature review and detailed techno-economic modeling for 2010 and 2020 in five locations, we provide such an assessment for the three currently leading large-scale solar technologies. We show that today these technologies cannot yet compete with conventional forms of power generation but approach competitiveness around 2020 in favorable locations. Furthermore, from a global perspective we find that none of the solar technologies emerges as a clear winner and that cost of storing energy differs by technology and can change the order of competitiveness in some instances. Importantly, the competitiveness of the different technologies varies considerably across locations due to differences in, e.g., solar resource and discount rates. Based on this analysis, we discuss policy implications with regard to fostering the diffusion of solar technologies while increasing the efficiency of policy support through an adequate geographical allocation of solar technologies. 相似文献
60.