Kinetic and spectral aspects in chemiluminescence system Eu(III)/HCO3/H2O2

Chemiluminescence (CL) of the systems containing Eu₂⁺ or HCO₃⁻ ions and hydrogen peroxide was studied. The kinetic curves and CL emission spectra of the systems were discussed. The emission spectrum of the system containing carbonates revealed two emission maxima: the first directly after initiation of the reaction by hydrogen peroxide and the second in several seconds after the first. On the basis of the characteristic bands in the CL spectrum the emitters in the system Eu₂⁺/HCO₃⁻/H₂O₂ were identified as: excited Eu³⁺ ions and excited products of carbonate decomposition. The emission bands for λ=600 and 420 nm appeared in a time distance of a few ten seconds.     

Antioxidant vs. Prooxidant Properties of the Flavonoid,Kaempferol, in the Presence of Cu(II) Ions: A ROS-Scavenging Activity,Fenton Reaction and DNA Damage Study

Kaempferol is a flavonoid that occurs in tea and in many vegetables and fruits, including broccoli, cabbage, beans, grapes, apples, and strawberries. The efficacy of Kaempferol has been demonstrated in the treatment of breast, esophageal, cervical, ovarian, and liver cancers and leukemia, which very likely arises from its prooxidant properties and the activation of pro-apoptotic pathways. Indeed, this matter has already been the focus of a number of published studies and reviews. The aim of the present study was to elucidate the antioxidant vs. prooxidant properties of flavonoids in the presence of the redox-active metal, copper (II) ion, by means of the Fenton reaction. The specific motivation of this work is that, since an increased level of Cu(II) ions is known to be associated with many disease states such as neurological conditions (Alzheimer’s disease) and cancer, any interaction between these ions and flavonoids might affect the outcome of therapeutic uses of the latter. The structure of the Cu-kaempferol complex in DMSO was investigated by means of low temperature EPR spectroscopy, which confirmed the existence of at least two distinct coordination environments around the copper (II) ion. UV vis-spectra of kaempferol and its Cu(II) complex in DMSO revealed an interaction between the 5-OH (A ring) group and the 4-CO (C ring) group of kaempferol with Cu(II) ions. An ABTS assay confirmed that kaempferol acted as an effective radical scavenger, and that this effect was further enhanced in the form of the Cu(II)-kaempferol complex. Quantitative EPR spin trapping experiments, using DMPO as the spin trap, confirmed suppression of the formation of a mixture of hydroxyl, superoxide, and methyl radicals, in a Fenton reaction system, upon coordination of kaempferol to the redox-active Cu(II) ions, by 80% with respect to the free Cu(II) ions. A viscometric study revealed a better DNA-intercalating ability of the Cu-kaempferol complex than for free kaempferol, essential for conferring anticancer activity of these substances. The results of the viscometric measurements were compared with those from a DNA damage study of Cu-kaempferol complexes in a Fenton reaction system, using gel electrophoresis. At low concentrations of kaempferol (Cu–kaempferol ratios of 1:1 and 1:2), a very weak protective effect on DNA was noted, whereas when kaempferol was present in excess, a significant DNA-protective effect was found. This can be explained if the weakly intercalated kaempferol molecules present at the surface of DNA provide protection against attack by ROS that originate from the Fenton reaction involving intercalated Cu(II)-kaempferol complexes. Following the application of ROS scavengers, L-histidine, DMSO, and SOD, gel electrophoresis confirmed the formation of singlet oxygen, hydroxyl radicals, and superoxide radical anions, respectively. We propose that the prooxidant properties of Cu-kaempferol complexes may provide anticancer activity of these substances. When present in excess, kaempferol displays antioxidant properties under Cu-Fenton conditions. This suggests that kaempferol might prove a suitable candidate for the prevention or treatment of oxidative stress related medical conditions that involve a disturbed metabolism of redox metals such as copper, for example, Menkes disease, and neurological disorders, including Alzheimer’s disease. For the potential use of kaempferol in clinical practice, it will be necessary to optimize the dose size and critical age of the patient so that this flavonoid may be beneficial as a preventive drug against cancer and neurological disorders.     

Stretching and deformation vibrations of CH2, C(CH3) and O(CH3) groups of poly(methyl methacrylate)

Infrared and Raman spectra of atactic PMMA and infrared spectra of stereoregular PMMA and of its four deuterated derivatives

, were measured. They were used to assign the bands of stretching and deformation vibrations of CH₂, CCH₃ and OCH₃ groups in the infrared and Raman spectra and to discuss the effect of stereoregularity on these bands in the infrared spectra.     

Investigation into etching mechanism of polyethylene terephthalate (PET) films treated in helium and oxygenated‐helium atmospheric plasmas

This research makes an investigation into the etching mechanism of atmospheric plasma conditions on the surface of polyethylene terephthalate (PET) films. Two types of untreated PET films (S/200 and S/500) were exposed to plasma for 0 to 5.0 min in 30‐s increments. The first set of each film type was treated in helium plasma, while the second was treated in oxygenated‐helium plasma. Differential Scanning Calorimetry (DSC) was used to characterize pre‐ and post‐exposure films. Weight changes and the degree of solubility were also determined. Based on peak area results, the percent crystallinity of PET S/200 increased by an average of 4.57% (helium treated) and 13.56% (oxygenated‐helium treated), while the S/500 showed only a small increase. There was no significant change in the melting or crystallization temperatures of either film type, indicating a decrease in amorphous content versus an increase in crystalline material. Weight loss analysis supports this theory. Solubility testing revealed a continual decrease in swelling as exposure time was increased. A model was developed to predict the change in the degree of solubility for polyphase surfaces considering the etching rate per phase. The model was applied to PET with good correlation between the model and experimental data. © 2004 Wiley Periodicals, Inc. J Appl Polym Sci 94: 2383–2389, 2004     

‘<Emphasis Type="Italic">if p then q</Emphasis>’ . . . and all that: Logical Elements in Reasoning and Discourse

In this paper we explore differences in use of the so-called ‘logical’ elements of language such as quantifiers and conditionals, and use this to explain differences in performance in reasoning tasks across subject groups with different educational backgrounds. It is argued that quantified sentences are difficult natural bases for reasoning, and hence more prone to elicit variation in reasoning behaviour, because they are chiefly used with a pre-determined domain in everyday speech. By contrast, it is argued that conditional sentences form natural premises because of the function they serve in everyday speech. Implications of this for the role of logic in modelling human reasoning behaviour are briefly considered.     

Functional polysiloxanes

Summary Carboxyester- and carboxyamido-terminated polydimethylsiloxanes were obtained through the reaction of cyclic anhydrides (succinic, maleic and phthalic anhydride) with hydroxypropyl- and aminoalkyl-terminated polydimethylsiloxanes, respectively. The reactivity of the starting compounds and the influence of the reaction conditions on the characteristics of the final products are discussed.Part 1: Eur. Polym. J., in press     

The smart car seat: personalized monitoring of vital signs in automotive applications

Embedded wireless sensors are important components of mobile distributed computing networks, and one of the target applications areas is health care. The preservation of mobility for senior citizens is one of the key issues in maintaining an independent lifestyle. Thus health technologies inside a car can contribute both to safety issues (supervision of driver fitness) as well as healthcare issues by monitoring vitals signs imperceptibly. In this paper, three embedded measurement techniques for non-contact monitoring of vital signals have been investigated. Specifically, capacitive electrocardiogram (cECG) monitoring, mechanical movement analysis (ballistocardiogram, BCG) using piezo-foils and inductive impedance monitoring were examined regarding their potential for integration into car seats. All three sensing techniques omit the need for electroconductive contact to the human body, but require defined mechanical boundary conditions (stable distances or, in the case of BCG, frictional connection). The physical principles of operation, the specific boundary conditions regarding automotive integration and the results during wireless operation in a running car are presented. All three sensors were equipped with local intelligence by incorporating a microcontroller. To eliminate the need for additional cabling, a wireless Bluetooth communication module was added and used to transmit data to a measurement PC. Finally, preliminary results obtained during test drives on German city roads and highways are discussed.     

Organizational Norm Congruency and Interpersonal Familiarity in E‐Mail: Examining Messages From Two Different Status Perspectives

Relying on SIDE theory, this 2 × 2 experimental design tested the effects of adherence to organizational norms and interpersonal familiarity in e‐mail messages from both superior and subordinate perspectives. Results reveal that using norm‐congruent e‐mail messages account for over 50% of the variance in both superior and subordinate attitudes toward the message and between 30% and 56% of the variance in perceptions of source credibility. Data from the superiors indicate that norm congruent e‐mail messages account for 14.4% of the variance in message compliance. Results from subordinates reveal an interaction effect that is consistent with SIDE‐based predictions. Subordinates believe superiors are most likely to comply with e‐mail requests from unfamiliar subordinates that are crafted in a manner congruent with organizational norms.     

Exploring creative thinking in graphically mediated synchronous dialogues

This paper reports on an aspect of the EC funded Argunaut project which researched and developed awareness tools for moderators of online dialogues. In this study we report on an investigation into the nature of creative thinking in online dialogues and whether or not this creative thinking can be coded for and recognized automatically such that moderators can be alerted when creative thinking is occurring or when it has not occurred after a period of time. We outline a dialogic theory of creativity, as the emergence of new perspectives from the interplay of voices, and the testing of this theory using a range of methods including a coding scheme which combined coding for creative thinking with more established codes for critical thinking, artificial intelligence pattern-matching techniques to see if our codes could be read automatically from maps and ‘key event recall’ interviews to explore the experience of participants. Our findings are that: (1) the emergence of new perspectives in a graphical dialogue map can be recognized by our coding scheme supported by a machine pattern-matching algorithm in a way that can be used to provide awareness indicators for moderators; (2) that the trigger events leading to the emergence of new perspectives in the online dialogues studied were most commonly disagreements and (3) the spatial representation of messages in a graphically mediated synchronous dialogue environment such as Digalo may offer more affordance for creativity than the much more common scrolling text chat environments. All these findings support the usefulness of our new account of creativity in online dialogues based on dialogic theory and demonstrate that this account can be operationalised through machine coding in a way that can be turned into alerts for moderators.     

A Pattern-Matching Compiler

Implementation of a rule-based transformation engine consists of several tasks with various abstraction levels. We present a new tool called mtom for the efficient implementation of rule-based transformations. This engine should help to bridge the gap between rewriting implementations and practical applications. It aims at implementing well-identified parts of complex applications where the use of rewriting is natural or crucial. These parts are specified using rewrite rules and integrated with the rest of the application, which is kept in a classical imperative language such as C, C++ or Java. Our tool, which can be viewed as a Yacc-like pre-processor, does not depend on a given term representation, rather it accepts implementation of terms (or term like data-types) of yet existing applications and it permits to define and execute rewrite rules upon those types. From our experiences, this system is well-suited for industrial use as well as for implementations of rule-based languages. The paper introduces several features supported by mtom.