The impact of perceptual treatments on lateral control: A study using fixed-base and motion-base driving simulators

This research was carried out as part of the French national multidisciplinary research project, PREDIT-SARI. Using a driving simulator, it aimed to test the effectiveness of road treatments intended to inform drivers about the risk of losing control on rural roads with “crest vertical curves” (Landis et al., 2004). [Rosey, F., Auberlet, J.M., Bertrand, J., Plainchault, P., 2008. Impact of perceptual treatments on lateral control during driving on crest vertical curves: a driving simulator study. Accid. Anal. Prev. 40, 1515-1523, Scopus.] used a fixed-base driving simulator to test four perceptual treatments intended to help drivers maintain lateral control when driving on crest vertical curves and found that two of them, rumble strips on both sides of the centerline and sealed shoulders, were more effective than the others. This first study prompted us to ask if non-visual sensory cues (e.g., vestibular or proprioceptive perceptions) could influence driver perception and consequently lateral control. We therefore conducted a second study on a motion-base driving simulator, using the same virtual 3D database. The results showed that: (1) drivers drive closer to the center of their lane when there are rumble strips on both sides of the centerline, or when there are sealed shoulders, than they do with the current marking system (i.e., continuous lines), and (2) the impact of the two tested perceptual treatments was replicated on both types of driving simulator.     

Examination of the working memory components in normal aging and in dementia of the Alzheimer type

The verbal and attentional components of working memory were examined in patients with Alzheimer's disease, normal elderlies, and young controls. Patients with Alzheimer's disease, showed a reduced span but were sensitive to word length. This is indicative of a functional rehearsal procedure. However, the effect of phonological similarity on immediate recall was smaller in patients with Alzheimer's disease and these patients showed a depressed performance in tasks of phonological analysis. There was also a significant decrement in a task that assessed the attentional component of working memory. Examination of individual patterns of performance showed that the phonological deficiency was severe in a subgroup of patients while the attentional deficit was more general.     

At Embryo Implantation Site IL-35 Secreted by Trophoblast,Polarizing T Cells towards IL-35+ IL-10+ IL-4+ Th2-Type Cells,Could Favour Fetal Allograft Tolerance and Pregnancy Success

We investigated the role of rhIL-35, at low concentrations compatible with those produced by human trophoblast cells (less than 1 ng/mL), on human T helper (Th) cell functions and the presence of decidual IL-35-producing Th cells in human pregnancy. We found that human trophoblast cells produced IL-35 but not IL-4 or IL-10. RhIL-35, at concentrations produced by human trophoblasts, polarized T cells towards IL-35+, IL-10+, IL-4+ Th2-type cells and to Foxp3+ EBI3+ p35+ T reg cells producing IL-35 but not IL-10 and IL-4. Moreover, rhIL-35 at low concentrations did not suppress the proliferation of Th cells but stimulated IL-4 and IL-10 production by established Th clones. In particular, Th1-type clones acquired the capacity to produce IL-4. In addition, purified human trophoblast cell supernatants containing IL-35 upregulated IL-4 and IL-10 production by Th clones. Finally, IL-35+, IL-10+, IL-4+ Th2-type cells, which were found to be induced by low concentrations of IL-35 compatible with those produced by human trophoblasts, are exclusively present in the decidua of a successful pregnancy and at the embryo implantation site, suggesting their stringent dependence on trophoblast cells. Thus, the proximity of Th cells to IL-35-producing trophoblasts could be the determining factor for the differentiation of IL-35+, IL-10+, IL-4+ Th2-type cells that are crucial for human pregnancy success.     

Fuzzy Logic Analysis of Alumina-Titania Deposition Yield During APS Process

The behavior modeling of Atmospheric Plasma Spray (APS) process requires a global approach which considers interrelated non-linear relationships between coating characteristics/properties in-service and process parameters (power, feedstock injection, kinematics, etc.). Such an approach would permit to reduce the development costs. To reach this objective, the knowledge of the interactions between process parameters plays a relevant role in the optimization. This work intends to develop a behavior model based on fuzzy logic concepts. Here, the model considers the deposition yield as the result of the process and it establishes relationships with power process parameter (arc current intensity, plasma gas total flow rate, hydrogen content) on the basis of fuzzy rules. The model hence permits to discriminate the role and the effects of each power process parameters. The modeling results are compared to experimental data. The specific case of the deposition of alumina-titania (Al₂O₃-TiO₂, 13% by weight) by Atmospheric Plasma Spraying (APS) is considered. This article is an invited paper selected from presentations at the 2007 International Thermal Spray Conference and has been expanded from the original presentation. It is simultaneously published in Global Coating Solutions, Proceedings of the 2007 International Thermal Spray Conference, Beijing, China, May 14-16, 2007, Basil R. Marple, Margaret M. Hyland, Yuk-Chiu Lau, Chang-Jiu Li, Rogerio S. Lima, and Ghislain Montavon, Ed., ASM International, Materials Park, OH, 2007.     

Evaluation of hexavalent chromium extraction method EPA method 3060A for soils using XANES spectroscopy

Hexavalent chromium (Cr(VI)) occurrence in soils is generally determined using an extraction step to transfer it to the liquid phase where it is more easily detected and quantified. In this work, the performance of the most common extraction procedure (EPA Method 3060A) using NaOH-Na(2)CO(3) solutions is evaluated using X-ray absorption near edge structure spectroscopy (XANES), which enables the quantification of Cr(VI) directly in the solid state. Results obtained with both methods were compared for three solid samples with different matrices: a soil containing chromite ore processing residue (COPR), a loamy soil, and a paint sludge. Results showed that Cr(VI) contents determined by the two methods differ significantly, and that the EPA Method 3060A procedure underestimated the Cr(VI) content in all studied samples. The underestimation is particularly pronounced for COPR. Low extraction yield for EPA Method 3060A was found to be the main reason. The Cr(VI) present in COPR was found to be more concentrated in magnetic phases. This work provides new XANES analyses of SRM 2701 and its extraction residues for the purpose of benchmarking EPA 3060A performance.     

Nanodiamond as a vector for siRNA delivery to Ewing sarcoma cells

The ability of diamond nanoparticles (nanodiamonds, NDs) to deliver small interfering RNA (siRNA) into Ewing sarcoma cells is investigated with a view to the possibility of in-vivo anticancer nucleic-acid drug delivery. siRNA is adsorbed onto NDs that are coated with cationic polymer. Cell uptake of NDs is demonstrated by taking advantage of the NDs' intrinsic fluorescence from embedded color-center defects. Cell toxicity of these coated NDs is shown to be low. Consistent with the internalization efficacy, a specific inhibition of EWS/Fli-1 gene expression is shown at the mRNA and protein level by the ND-vectorized siRNA in a serum-containing medium.     

Volatile metal species in coal combustion flue gas

Metals are released in effluents of most of combustion processes and are under intensive regulations. To improve our knowledge of combustion process and their resulting emission of metal to the atmosphere, we have developed an approach allowing usto distinguish between gaseous and particulate state of the elements emitted. This study was conducted on the emission of volatile metallic species emitted from a coal combustion plant where low/medium volatile coal (high-grade ash) was burnt. The occurrence of volatile metal species emission was investigated by cryofocusing sampling procedure and detection using low-temperature packed-column gas chromatography coupled with inductively coupled plasma-mass spectrometry as multielement detector (LT-GC/ICP-MS). Samples were collected in the stack through the routine heated sampling line of the plant downstream from the electrostatic precipitator. The gaseous samples were trapped with a cryogenic device and analyzed by LT-GC/ICP-MS. During the combustion process, seven volatile metal species were detected: three for Se, one for Sn, two for Hg, and one for Cu. Thermodynamic calculations and experimental metal species spiking experiments suggest that the following volatile metal species are present in the flue gas during the combustion process: COSe, CSSe, CSe2, SeCl2, Hg0, HgCl2, CuO-CuSO4 or CuSO4 x H2O, and SnO2 or SnCl2. The quantification of volatile species was compared to results traditionally obtained by standardized impinger-based sampling and analysis techniques recommended for flue gas combustion characterization. Results showed that concentrations obtained with the standard impinger approach are at least 10 times higher than obtained with cryogenic sampling, suggesting the trapping microaerosols in the traditional methods. Total metal concentrations in particles are also reported and discussed.     

Sexual Dimorphism in Glucocorticoid Stress Response

Chronic stress is encountered in our everyday life and is thought to contribute to a number of diseases. Many of these stress-related disorders display a sex bias. Because glucocorticoid hormones are the main biological mediator of chronic stress, researchers have been interested in understanding the sexual dimorphism in glucocorticoid stress response to better explain the sex bias in stress-related diseases. Although not yet demonstrated for glucocorticoid regulation, sex chromosomes do influence sex-specific biology as soon as conception. Then a transient rise in testosterone start to shape the male brain during the prenatal period differently to the female brain. These organizational effects are completed just before puberty. The cerebral regions implicated in glucocorticoid regulation at rest and after stress are thereby impacted in a sex-specific manner. After puberty, the high levels of all gonadal hormones will interact with glucocorticoid hormones in specific crosstalk through their respective nuclear receptors. In addition, stress occurring early in life, in particular during the prenatal period and in adolescence will prime in the long-term glucocorticoid stress response through epigenetic mechanisms, again in a sex-specific manner. Altogether, various molecular mechanisms explain sex-specific glucocorticoid stress responses that do not exclude important gender effects in humans.