Three-Dimensional Structural Aspects of Protein–Polysaccharide Interactions

Linear polysaccharides are typically composed of repeating mono- or disaccharide units and are ubiquitous among living organisms. Polysaccharide diversity arises from chain-length variation, branching, and additional modifications. Structural diversity is associated with various physiological functions, which are often regulated by cognate polysaccharide-binding proteins. Proteins that interact with linear polysaccharides have been identified or developed, such as galectins and polysaccharide-specific antibodies, respectively. Currently, data is accumulating on the three-dimensional structure of polysaccharide-binding proteins. These proteins are classified into two types: exo-type and endo-type. The former group specifically interacts with the terminal units of polysaccharides, whereas the latter with internal units. In this review, we describe the structural aspects of exo-type and endo-type protein-polysaccharide interactions. Further, we discuss the structural basis for affinity and specificity enhancement in the face of inherently weak binding interactions.     

Effect of conspecific and heterospecific feces on foraging and oviposition of two predatory ladybirds: role of fecal cues in predator avoidance

Growing evidence suggests a flow of chemical information from higher to lower trophic levels that affects foraging and oviposition of prey in response to potential risks from predators. This was investigated in two species of ladybird predators of aphids, Harmonia axyridis and Propylea japonica. H. axyridis is known to be the stronger intraguild predator and P. japonica to be the more frequent intraguild prey in interactions of these two species. These ladybirds share aphid prey on mugworts, hibiscus, and Italian ryegrasses in fields of northern Japan but largely avoid each other on the same plant. Fecal cues of these ladybird predators were found to contribute in their assessment of predation risk from conspecific and heterospecific competitors in common habitats. Gravid females of H. axyridis reduced rates of feeding and oviposition when exposed to feces of conspecifics, but not when exposed to feces of P. japonica. In contrast, gravid females of P. japonica reduced feeding and oviposition when exposed to feces of both H. axyridis and its own species. Females of both ladybird species exhibited similar behavior in response to water extracts of feces. For P. japonica, the influence of heterospecific feces was greater than that of conspecific feces. Our results demonstrate that feces of ladybirds contain odors that have the potential to deter the feeding and oviposition activities of conspecific as well as heterospecific ladybirds. Such deterrence allows these insects to avoid predation risk. Differences in responses of the two predators are discussed.     

Genomic Instability and Cancer Risk Associated with Erroneous DNA Repair

Many cancers develop as a consequence of genomic instability, which induces genomic rearrangements and nucleotide mutations. Failure to correct DNA damage in DNA repair defective cells, such as in BRCA1 and BRCA2 mutated backgrounds, is directly associated with increased cancer risk. Genomic rearrangement is generally a consequence of erroneous repair of DNA double-strand breaks (DSBs), though paradoxically, many cancers develop in the absence of DNA repair defects. DNA repair systems are essential for cell survival, and in cancers deficient in one repair pathway, other pathways can become upregulated. In this review, we examine the current literature on genomic alterations in cancer cells and the association between these alterations and DNA repair pathway inactivation and upregulation.     

Oxytocin-Dependent Regulation of TRPs Expression in Trigeminal Ganglion Neurons Attenuates Orofacial Neuropathic Pain following Infraorbital Nerve Injury in Rats

We evaluated the mechanisms underlying the oxytocin (OXT)-induced analgesic effect on orofacial neuropathic pain following infraorbital nerve injury (IONI). IONI was established through tight ligation of one-third of the infraorbital nerve thickness. Subsequently, the head withdrawal threshold for mechanical stimulation (MHWT) of the whisker pad skin was measured using a von Frey filament. Trigeminal ganglion (TG) neurons innervating the whisker pad skin were identified using a retrograde labeling technique. OXT receptor-immunoreactive (IR), transient receptor potential vanilloid 1 (TRPV1)-IR, and TRPV4-IR TG neurons innervating the whisker pad skin were examined on post-IONI day 5. The MHWT remarkably decreased from post-IONI day 1 onward. OXT application to the nerve-injured site attenuated the decrease in MHWT from day 5 onward. TRPV1 or TRPV4 antagonism significantly suppressed the decrement of MHWT following IONI. OXT receptors were expressed in the uninjured and Fluoro-Gold (FG)-labeled TG neurons. Furthermore, there was an increase in the number of FG-labeled TRPV1-IR and TRPV4-IR TG neurons, which was inhibited by administering OXT. This inhibition was suppressed by co-administration with an OXT receptor antagonist. These findings suggest that OXT application inhibits the increase in TRPV1-IR and TRPV4-IR TG neurons innervating the whisker pad skin, which attenuates post-IONI orofacial mechanical allodynia.     

Adsorbed Atoms and Molecules Destined for a Reaction

The idea of an activation complex is popular for explaining reaction rates, but the characteristics of reactions and catalysis may not be explained in this way. A predestined state for each reaction composed of surface atoms and adsorbed species is responsible for these features. Two single Sn atoms trapped in adjacent half-unit cells of an Si(111) 7 × 7 surface is an example of a predestined state. An isolated Sn atom in a half-unit cell does not migrate to other half-unit cells at room temperature, but when two single Sn atoms are in adjacent half-unit cells they undergo rapid combination to form an Sn₂ dimer. In addition, these two single Sn atoms replace the center Si adatoms and an Si₄ cluster is formed. The spatial distribution of molecules desorbing from surfaces may reflect the predestined states for the desorption processes. The spatial distribution in the temperature-programmed desorption (TPD) of NO on Pd(110) and Pd(211) surfaces and that in the temperature-programmed reaction (TPR) of NO + H₂ were studied. N₂ desorbing from Pd(110) by the recombination of N atoms obeys cos⁶ – cos⁷ but the N₂ produced by a catalytic reaction of NO with H₂ obeys cos. In contrast, the N₂ desorbing with NO at 490 K in the TPD of Pd(110) shows a sharp off-normal distribution expressed by cos⁴⁶( – 38). The adsorption of NO on Pd(211) predominantly occurs on the (111) terrace but the spatial distribution suggests that the predestined states for the reaction and desorption are formed on both the (111) terrace and (100) step surfaces.     

Solidification by Convective Drying of Particle Layer Wetted with Dilute Agar Gel

Solidified porous slab is formed through convective drying of glass particle layer wetted with aqueous dilute agar gel. Measured critical mean moisture content increases with increasing initial moisture or agar content. The agar gel moves in viscous flow caused by capillary pressure during drying. A new drying model based on the receding evaporation plane model is proposed. Drying period is divided into surface and internal evaporation periods. Wet slab consists of dried and wet zones during the internal evaporation period, while the wet slab consists of wet zone only during the surface evaporation period. In the new model, the evaporation rate from the wet zone in the internal drying period is estimated with the linear driving force (LDF) approximation in the field of adsorption engineering. Critical moisture content, that is, mean moisture content between the surface and internal periods, is estimated with a mass balance on the surface. Simulated results by the new drying model with reasonable fitting parameters agree very well with measured drying data.     

A switching command-based whole-body operation method for humanoid robots

This paper introduces a switching command-based whole-body operation method for humanoid robots. Humanoid robots are biped machines possessing multiple degrees of freedom (DOF). Due to the complexity of their multi-DOF structure, and the difficulty in maintaining postural stability, whole-body operation of humanoid robots is fundamentally different from traditional fixed-base manipulators or stable-base mobile manipulators. By studying the shifts in locus of attention between human body joints during task execution, we developed a switching command-based operation method that allows the operator to select only the necessary points of the humanoid robot's body for manipulation. Whole-body motion satisfying the desired movements of the selected points is generated using an inverse-kinematics motion generation scheme. This switching operation method enables flexible whole-body operation of humanoid robots using simple input devices. The proposed whole-body operation method is implemented as a teleoperation system using two 3-DOF joysticks to operate a 30-DOF humanoid robot (HRP-1S) developed in the Humanoid Robotics Project (HRP) of the Ministry of Economy, Trade, and Industry of Japan. Experiments teleoperating HRP-1S confirmed the effectiveness of our method.     

Content Provisioning for Ubiquitous Learning

In this article, the authors present an approach for context-aware and QoS-enabled learning content provisioning, one of the essential elements in ubiquitous learning. The essence of the system is recommending the right content, in the right form, to the right learner, based on a wide range of user context information and QoS requirements. To facilitate knowledge interoperability and sharing, they modeled the learner context, content knowledge, and domain knowledge using ontologies. They first propose a knowledge-based semantic recommendation method to acquire the content the user really wants and needs to learn. Then, a fuzzy logic-based decision-making strategy and an adaptive QoS mapping mechanism determine the appropriate presentation according to user's QoS requirements and device/network capability.     

Ultra-sensitive high-speed density measurement of the “piston effect” in a critical fluid

In order to conduct a detailed investigation of the piston effect, the peculiar heat transportation phenomenon in critical fluids, an ultra-sensitive high-speed density measurement system was developed using a very thin heater, a sensitive interferometer and a large-capacity high-speed data acquisition system. As the first step of the investigation, an experiment was conducted to measure the velocity of sound in CO2 near its critical point. Short heat pulses suppressing the disturbance of natural convection were applied to a small cell filled with almost-critical CO2 fluid and were detected by the measurement system. Because the pulses propagated through the cell at the velocity of sound and were reflected several times between the cell walls, the velocity could be precisely determined by measuring the time intervals between successive reflections. The resulting velocity profile versus temperature showed good agreement with theoretical prediction and numerical simulations. This result validated the density measurement system, and it is considered that the measurement system will be a very effective tool for further studies on critical phenomena with the aid of numerical simulation.