Disrupted temporal control in the R6/2 mouse model of Huntington’s disease.

Huntington’s disease is characterized by corticostriatal dysfunction and degeneration of the striatum with progressive loss of the medium spiny neurons. These circuits are important for instrumental responding, interval timing, and temporal control over motor output. We investigated the acquisition of timed operant responding in two R6/2 Huntington’s Disease models, differing in CAG repeat length and genetic background (115 and 250 CAG repeats, and a mixed CBAxC57 or pure C57 background) and their corresponding wild type controls using the peak procedure. Both mouse lines exhibited similar response control deficits. In unreinforced peak trials, mice either did not learn to terminate an ongoing response past reinforcement time or required more trials to acquisition compared to the wild type mice. While transgenic and wild type mice did not exhibit differences in temporal accuracy, response curves were flatter in transgenic mice, suggesting decreased temporal control over operant responding. The results are discussed in terms of the neurobiology of interval timing, instrumental responding, and the neuropathology of HD and R6/2 mice. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Elektronenstrahltesten: Genauigkeit von Zeitmessungen im ps-Bereich

Übersicht Die Entwicklung integrierter Mikrowellenschaltungen für analoge und digitale Anwendungen mit hohen Geschwindigkeiten erfordert neue Meßmethoden. Für die Prozeß- und Schaltungsentwicklung ist vor allem die belastungsfreie Messung interner Signale notwendig. Die bereits bewährte Elektronenstrahlmeßtechnik konnte diesen neuen Anforderungen angepaßt werden. Zeitkritische Größen sind sowohl Signalanstiegszeiten als auch Phasenlagen verschiedener Signale zueinander. Signalanstiegszeiten können gegenwärtig bis herab zu 15 ps mit einem Fehler unter 10% gemessen werden. Für Messungen von Phasenlagen zwischen Signalen, die innerhalb von 3 Stunden aufgezeichnet wurden, ergibt sich eine Genauigkeit von±2 ps.

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Electron-beam testing: accuracy of time measurements in the ps range

Contents The development of integrated microwave circuits for analogue and digital applications with higher speeds requires new testing methods. The process and circuit development primarily require non loding measurements of internal signals. The adaptation of the proven technique of e-beam testing to these new requirements was successfull. Signal rise times as well as phase relations between different signals are critical quantities in the time scale. Presently, rise times can be measured down to 15 ps with an error of less than 10%. Measurements between signals being recorded within 3 hours are possible with an accuracy of±2 ps.

     

Approximation algorithms for shop scheduling problems with minsum objective: A correction

We present a correction to the paper, “Approximation algorithms for shop scheduling problems with minsum objective” (Journal of Scheduling 2002; 5:287–305) by Queyranne and Sviridenko. This correction provides a correct derivation of its 2eρ approximation result. Wenhua Li and Jinjiang Yuan: Project supported by NNSFC (Grant 10371112) and NSFHN (Grant 0411011200). Maurice Queyranne: Supported by research grants from NSERC, the Natural Sciences and Engineering Research Council of Canada.     

Computational methods for database repair by signed formulae*

We introduce a simple and practical method for repairing inconsistent databases. Given a possibly inconsistent database, the idea is to properly represent the underlying problem, i.e., to describe the possible ways of restoring its consistency. We do so by what we call signed formulae, and show how the ‘signed theory’ that is obtained can be used by a variety of off-the-shelf computational models in order to compute the corresponding solutions, i.e., consistent repairs of the database. *This paper is a revised and extended version of [9].     

Pulsed Laser Deposition of Zr1-xCexO2 and Ce1-xLaxO2-x/2 for Buffer Layers and Insulating Barrier in Oxide Heterostructures

We have studied the pulsed laser deposition (PLD) of Zr_1-xCexO₂ and Ce_1-xLa_xO₂-x/2 initially to grow buffer layers for perovskite films deposited on Si, LaAlO₃, SrTiO₃ and MgO and then to produce tunneling barriers for cuprate or manganite heterostructures. On (1 0 0) Si, the deposition of Zr_1-xCexO₂ (x = 0.12) produces a smooth epitaxial layer (R _RMS = 0.25/1m²), which allows the further deposition of high quality YBa₂Cu₃O₇ (Tc 88 K) and La_0.7Sr_0.3MnO₃ films. On the other hand, the use of Ce_1-xLa_xO_2-x/2 (0 x 0.4) makes it possible to match the YBa₂Cu₃O₇ and La_0.7Sr_0.3MnO₃ layers to various substrates. The buffer layers are epitaxially grown with a 45° rotation of the in-plane axes with respect to those of the substrate, and the smoothness is high (R _RMS = 0.25/1m²). In the case of an ultra-thin barrier (2.5 nm) of Ce_1-xLa_xO_2-x/2 sandwiched in a La_0.7Sr_0.3MnO₃, the out-of-plane mismatch of 2 induces distortions at the interface steps, which propagate into the topmost La_0.7Sr_0.3MnO₃ layer. This is in contrast to the case of SrTiO₃ barriers where an ideal crystal continuity in the growth direction is observed.     

Clinical application of volume estimation based on three-dimensional ultrasonography

The development of three-dimensional sonography represents a milestone in the history of diagnostic ultrasound. It has become possible to evaluate a region of interest not only in the horizontal and vertical planes but also in a third, i.e. the frontal, plane. Among other possible applications, this technique greatly facilitates volume estimation. The objective of the present study was to evaluate the accuracy and precision of volumetry based on three-dimensional sonography. The results demonstrate a good correlation between estimated volumes and the volumes measured by transvaginal needle-guided follicle aspiration performed under sonographic visualization. Our findings confirm the conclusion of previous studies that three-dimensional sonography is making a valuable contribution to imaging techniques.     

A systolic array for the longest common subsequence problem

We introduce a linear systolic array for the Longest Common Subsequence (LCS, for short) problem. We first present an array of m identical cells which computes the length of an LCS of two strings of length m and n, respectively, in linear time (i.e., in time proportional to m + n). Then we show that, by extending any cell with the systolic stack introduced by Guibas and Liang (1982), a new array can be designed to recover an LCS in linear time.     

NAD+-dependent glyceraldehyde-3-phosphate dehydrogenase from Thermoproteus tenax. The first identified archaeal member of the aldehyde dehydrogenase superfamily is a glycolytic enzyme with unusual regulatory properties

The hyperthermophilic archaeum Thermoproteus tenax possesses two glyceraldehyde-3-phosphate dehydrogenases differing in cosubstrate specificity and phosphate dependence of the catalyzed reaction. NAD+-dependent glyceraldehyde-3-phosphate dehydrogenase catalyzes the phosphate-independent irreversible oxidation of D-glyceraldehyde 3-phosphate to 3-phosphoglycerate. The coding gene was cloned, sequenced, and expressed in Escherichia coli. Sequence comparisons showed no similarity to phosphorylating glyceraldehyde-3-phosphate dehydrogenases but revealed a relationship to aldehyde dehydrogenases, with the highest similarity to the subgroup of nonphosphorylating glyceraldehyde-3-phosphate dehydrogenases. The activity of the enzyme is affected by a series of metabolites. All effectors tested influence the affinity of the enzyme for its cosubstrate NAD+. Whereas NADP(H), NADH, and ATP reduce the affinity for the cosubstrate, AMP, ADP, glucose 1-phosphate, and fructose 6-phosphate increase the affinity for NAD+. Additionally, most of the effectors investigated induce cooperativity of NAD+ binding. The irreversible catabolic oxidation of glyceraldehyde 3-phosphate, the control of the enzyme by energy charge of the cell, and the regulation by intermediates of glycolysis and glucan degradation identify the NAD+-dependent glyceraldehyde-3-phosphate dehydrogenase as an integral constituent of glycolysis in T. tenax. Its regulatory properties substitute for those lacking in the reversible nonregulated pyrophosphate-dependent phosphofructokinase in this variant of the Embden-Meyerhof-Parnas pathway.