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101.
102.
MD. Shahidul Ahsan Satoshi Maruyama Jun Cheng Kamal Al‐Eryani Manabu Yamazaki Mayumi Hasegawa Masayuki Tsuneki Takashi Saku 《Microscopy research and technique》2011,74(3):264-268
For histopathological assessment of oral borderline malignancies, it is important to carefully detect subtle epithelial changes on fully stretched tissue sections. However, it is not generally easy to obtain wrinkle‐free sections when using formalin‐fixed paraffin‐embedded oral mucosal samples. Since acetic acid treatment is already utilized for large brain tissue sections, we examined whether that treatment was also effective for oral mucosal tissues containing normal to malignant epithelial lesions. Paraffin sections were floated in various concentrations of acetic acid for 10 min after stretching in water for 1 min, then wrinkle formations were examined using hematoxylin and eosin staining, as well as for staining intensity with keratin immunohistochemistry. Wrinkles were formed in both epithelial and connective tissue zones of sections treated with less than a 40‐mM (0.25%) concentration of acetic acid. In contrast, treatments with concentrations at 80 mM (0.5%) and higher resulted in cracking between the epithelial layer and lamina propria, as well as poor immunohistochemical results for keratins 13 and 17, even though the wrinkles completely disappeared. These results indicate that 40 mM is the optimal concentration of acetic acid solution to prevent wrinkles in the epithelial layer while maintaining the immunohistochemical qualities of oral mucosa tissue sections, especially those containing borderline malignant epithelial lesions. Microsc. Res. Tech., 2010. © 2010 Wiley‐Liss, Inc. 相似文献
103.
Takekazu Ishida Ikutaro Yagi Naohito Yoshioka Thanh Huy Ho Tsutomu Yotsuya Kazuo Satoh Mayumi Uno Hisashi Shimakage Shigehito Miki Zhen Wang Masaru Kato Masahiko Machida Kiichi Hojou 《Journal of Low Temperature Physics》2012,167(3-4):447-454
The MgB2 detector consists of 200?nm thick MgB2 thin film meander structure of 3?μm line width protected by 300?nm thick SiO layer. The electrical connections were provided by 150?nm thick Nb. The devices were placed in a 4?K refrigerator to investigate the behavior at temperatures below T c. The position-dependent response of the MgB2 detector can be investigated by scanning a laser spot with the aid of the XYZ piezo-driven stage and an optical fiber with a focusing lens. We found that the inhomogeneity in a detector is very critical to specify the detection efficiency since the detector has the sensitivity only in a narrow temperature regime near T c. 相似文献
104.
H Mayumi K Matsuzaki H Kohno K Matsui Y Kawachi H Yasui 《Canadian Metallurgical Quarterly》1996,20(7):810-814
To examine the function of the fallback I algorithm (Chorus I, Ela Medical Inc., Montrouge, France), which automatically changes the DDD mode to VDI during transient supraventricular tachyarrhythmias to prevent high-rate tracking of the venticule, a total of 45 patients who were preoperatively diagnosed with sick-sinus syndrome (SSS) (Group 1; n = 19) or with advanced or complete atrioventricular block (AVB) (Group 2; n = 26) were followed up and analyzed. Mean follow-up times (mean +/- SD) were 22.4 +/- 9.7 and 12.4 +/- 10.9 months, respectively. Each of the groups was further divided into subgroups according to the preoperative existence of of paroxysmal atrial fibrillation (PAF) or paroxysmal supraventricular tachyarrhythmia (PSVT). During follow-up, the fallback started through a given cycle of ventricular pacing at a upper-rate limit (URL) to avoid a continuous high-rate tracking during the arrhythmias specifically in the patients with PSVT (0/4) (p = 0.0004). The fallback, however, sometimes started in the patients who had AVB not associated with either PAF or PSVT (4/21) during normal exercise because the fallback algorithm did not distinguish a normal P wave from the abnormal atrial waves. To further clarify the behavior of the fallback, a treadmill test was conducted in 25 of the 45 patients. The fallback start was observed in 12 of the 17 patients with AVB (Group 2). In such patients, the use of a long fallback delay and/or a high URL setting prevented the fallback starts during normal exercise. These results suggest that, in DDD pacing, the algorithm in Chorus I is useful in patients with SSS or AVB to avoid highrate tracking of the ventricle during transient supraventricular tachyarrhythmias, but special care must be taken to avoid the fallback starts during the normal exercise in AVB patients. 相似文献
105.
Sasaki M Idiris A Tada A Kumagai H Giga-Hama Y Tohda H 《Yeast (Chichester, England)》2008,25(9):673-679
We report a gap-filling sequence between SPBPB21E7.09 (in contig c1348) and SPBPB10D8.01 (in contig pB10D8) on the left arm of chromosome 2 in the fission yeast, Schizosaccharomyces pombe. The sequence was determined from a BAC clone overlapping SPBPB21E7.01c (eno102) (in contig c1348) and SPBC1683.07 (mal1) (in contig pB10D8). The gap-filling sequence is 17,881 bp in length and contains five putative open reading frames, which were systematically named as SPBC460.01c, SPBC460.02c, SPBC460.03, SPBC460.04c and SPBC460.05. Their deduced amino acid sequences respectively include protein motifs corresponding to amino acid permease, glutathione S-transferase C-terminal domain, taurine catabolism dioxygenase TauD TfdA family and major facilitator superfamily, whereas their functions are unknown. 相似文献
106.
Kobayashi Y Hiroi T Araki M Hirokawa T Miyazawa M Aoki N Kojima T Ohsawa T 《Journal of the science of food and agriculture》2012,92(2):358-365
BACKGROUND: Tea made from Eucommia ulmoides leaves is widely consumed as a health food, since recent studies have revealed various pharmacological effects of the tea, e.g. a hypotriglyceridaemic effect. This study was conducted to elucidate the mechanisms underlying the plasma triglyceride‐lowering effect of E. ulmoides leaves. RESULTS: Rats were divided into four groups: a normal group, a group fed a high‐fat/high‐fructose diet (untreated group) and two groups fed a high‐fat/high‐fructose diet and E. ulmoides tea (4 or 20 g L?1 extract, treated groups). Plasma triglyceride concentrations were reduced in treated groups in a dose‐dependent manner compared with the untreated group. DNA microarray analysis revealed that genes involved in hepatic α‐, β‐ and ω‐oxidation, mainly related to the peroxisome proliferator‐activated receptor α and δ signalling pathway, were up‐regulated in the treated group. High‐performance liquid chromatography analysis showed that E. ulmoides leaves contain three phytochemicals that make up 60 mg g?1 of the material and are likely to be the active components. CONCLUSION: This study indicates that the promotion of fatty acid oxidation, probably by the action of phytochemicals, participates in the ameliorative effect of E. ulmoides leaves on hypertriglyceridaemia. These findings provide the scientific evidence for the functionality of E. ulmoides. Copyright © 2011 Society of Chemical Industry 相似文献
107.
Naoki Nishino Mayumi Taniguchi Ei Sakaguchi 《Journal of the science of food and agriculture》2001,81(11):1055-1059
The antinutritional activities of trypsin inhibitors (TIs) were compared between winged beans (Psophocarpus tetragonolobus) and soya beans (Glycine max). The inhibitors of the two beans were isolated by trypsin‐bound Sepharose 4B, and 50 mg of lyophilised powders were intubated intragastrically into 24 h fasted rats. The activities of trypsin and chymotrypsin were compared after 30, 60 and 180 min in the washings of the upper, middle and lower parts of the small intestine. The elution profiles of TI and non‐TI compounds in the affinity chromatography were similar in the two beans, and the antitryptic activities were concentrated 5.5 and 6.2 times (based on specific activity) for winged beans and soya beans respectively. Regardless of the TI fed to rats, trypsin activity in the upper intestine was suppressed to almost undetectable levels at 30 and 60 min after intubation. The activities in the middle and lower intestines were also substantially lowered when rats were fed winged bean TI, and significant differences were detected at 30 and 60 min after intubation when compared with rats fed soya bean TI. However, at 180 min after feeding, no differences were found in the trypsin activity in any gut segments. Similar inhibitory properties of isolated TIs were observed in chymotrypsin activities in the small intestine. The results suggest that winged bean TI may have greater inhibitory activity on the intestinal proteinase compared with soya bean TI. © 2001 Society of Chemical Industry 相似文献
108.
Makoto Kanauchi Ayaka Chijimi Mayumi Ohnishi‐Kameyama Charles W. Bamforth 《Journal of the Institute of Brewing》2013,119(1-2):32-40
Two enzymes with activity on xylan and xylan‐based substrates were isolated from malted barley. One appears to be relatively anionic and the other more cationic. Both are endo‐acting enzymes. The first of these was studied in more detail and shown to be of molecular weight ca. 59,000 and capable of acting through the pH range 5–7.5. It is relatively heat tolerant, with 30% of its activity surviving for 30 min at 70 °C. A low‐molecular‐weight (ca. 1300) inhibitor of xylanase that developed during germination was investigated. Its potency was not lowered by treatment with proteolytic enzymes, but treatment with polyvinylpolypyrrolidone did reduce its impact. Ultraviolet spectrophotometry and Fourier transform infrared spectroscopy, as well as staining with Folin–Ciocalteu reagent, indicated the presence of phenolic species, while mass spectroscopy studies suggested the presence of arabinose and possibly poly‐hexose. The presence of material that stained with aniline phthalate would be consistent with the presence of reducing sugar in the inhibitor. Copyright © 2013 The Institute of Brewing & Distilling 相似文献
109.
Dust absorption averaged over the Sahara inferred from moderate resolution imaging spectroradiometer
We estimated the average dust single scattering albedo (omega(0)) over the Sahara using four years of moderate resolution imaging spectroradiometer data. The method employed is based on the theory that the critical surface reflectance (rho(c)) for which the reflectance at the top-of-the-atmosphere is not influenced by the variability of dust optical thickness depends on omega(0). The average dust absorption over the Sahara was estimated to be smaller than that previously reported in the literature, and this may be causing the cooling of the climate system. Our method enables one to estimate omega(0) from data with a variety of aerosol optical thickness values using samples in the vicinity of rho(c). Use of satellite data over large areas and for long periods enables quantification of the average dust absorption over the entire Sahara. 相似文献
110.
In this paper, we particularly deal with no Fp‐rational two‐torsion elliptic curves, where Fp is the prime field of the characteristic p. First we introduce a shift product‐based polynomial transform. Then, we show that the parities of (#E – 1)/2 and (#E’ – 1)/2 are reciprocal to each other, where #E and #E’ are the orders of the two candidate curves obtained at the last step of complex multiplication (CM)‐based algorithm. Based on this property, we propose a method to check the parity by using the shift product‐based polynomial transform. For a 160 bits prime number as the characteristic, the proposed method carries out the parity check 25 or more times faster than the conventional checking method when 4 divides the characteristic minus 1. Finally, this paper shows that the proposed method can make CM‐based algorithm that looks up a table of precomputed class polynomials more than 10 percent faster. 相似文献