Identification of aminoreductones as active components in Maillard reaction mixtures inducing nuclear NF‐κB translocation in macrophages

Coffee, a highly processed food, and Maillard mixtures are able to activate nuclear factor κB translocation in macrophages via generation of hydrogen peroxide. In this study, a substructure library was prepared and used to identify Maillard products that are responsible for this effect. Three different Maillard reaction products with aminoreductone substructure (C₆‐aminoreductone, C₄‐aminoreductone, and aminohexose reductone) strongly induce nuclear factor κB translocation in macrophages. The effect was almost completely blocked by co‐incubation with catalase, indicating that cellular activation was mediated by the ability of the test compounds to generate hydrogen peroxide. The cellular effect of a Maillard mixture, which was produced under conditions favoring aminoreductone formation, could be almost completely related to the presence of C₆‐aminoreductone.     

Impact of enzymatic mash maceration and storage on anthocyanin and color retention of pasteurized strawberry purées

Strawberry purées were prepared using a commercial polygalacturonase (PG) and a highly purified pectinesterase (PE) preparation, respectively. To elucidate the effect of pectin on color stability following enzymatic pulp maceration, pectin composition was studied by isolating and fractionating the alcohol-insoluble residue from the strawberry purées. The purées were stored at +20 and +4 °C in the dark over a period of 24 weeks monitoring the amounts of monomeric and polymeric anthocyanins as well as antioxidant activities (FRAP, TEAC). Individual anthocyanins were analyzed by HPLC–DAD–MS ⁿ , and color measurements were obtained in the CIE L*a*b* system. Pectin composition was significantly modified following enzymatic maceration of the purées. While PG treatment generally resulted in pectin losses, oxalate-soluble pectins were increased in PE-treated purées. After 24 weeks of storage, the best anthocyanin retention was observed in PE-treated purées. Such products also revealed greatest anthocyanin half-life values and lowest proportion of polymeric pigments. Compared to an untreated control, enzymatic purée maceration using the PG was also beneficial for pigment retention, but less effective than PE. In contrast, color and antioxidant activity were independent of both enzymatic treatments. An initial heating step (90 °C, 10 s) for immediate inactivation of native enzymes such as polyphenoloxidases slightly improved pigment stability, while lowered temperature during mash maceration was less effective. However, by far best color and pigment retention were achieved when the purées were stored at 4 °C in the dark.     

Thermal inactivation of strawberry polyphenoloxidase and its impact on anthocyanin and color retention in strawberry (Fragaria x ananassa Duch.) purées

Strawberry (Fragaria x ananassa Duch.) cultivars were screened for their polyphenoloxidase (PPO) activities, and thermal stability of PPO was evaluated in vitro for three cultivars under different time–temperature regimes (60, 75 and 90 °C for 3 and 5 min, respectively). Heating strawberry purées should further elucidate the impact of thermal treatments on strawberry PPO in its natural matrix (‘in situ’ activity). To evaluate the consequences of PPO inactivation on anthocyanin and color stability, the purées were stored for 28 days at +20 °C monitoring the contents of monomeric, polymeric (spectrophotometrically) and individual anthocyanins (HPLC–DAD–MSⁿ) as well as color properties (CIE L*a*b*). Antioxidant activities (FRAP), total phenolic (Folin–Ciocalteu) and ascorbic acid contents of freshly prepared and stored purées, respectively, were determined spectrophotometrically. PPO activities varied considerably among the cultivars investigated. Accordingly, different time–temperature regimes were required for their complete in vitro and in situ inactivation. Unexpectedly, thermal inactivation of PPO was disadvantageous regarding pigment and color retention of strawberry purées, which was ascribed to partial regeneration of PPO. Hence, protection of antioxidants, total phenolics and ascorbic acid from oxidative degradation could not be achieved by heating the purées prior to storage.     

Occurrence of ochratoxin A in cocoa by-products and determination of its reduction during chocolate manufacture

This work reports an investigation carried out to assess the natural occurrence of ochratoxin A in 168 samples from different fractions obtained during the technological processing of cocoa (shell, nibs, liquor, butter, cake and cocoa powder) and the reduction of ochratoxin A during chocolate manufacture. Ochratoxin A analyses were performed with immunoaffinity columns and detection by high performance liquid chromatography. Concerning the natural ochratoxin A contamination in cocoa by-products, the highest levels of ochratoxin A were found in the shell, cocoa powder and cocoa cake. The cocoa butter was the least contaminated, showing that ochratoxin A seems to remain in the defatted cocoa solids. Under the technological conditions applied during the manufacture of chocolate in this study and the level of contamination present in the cocoa beans, this experiment demonstrated that 93.6% of ochratoxin A present in the beans was reduced during the chocolate producing.     

Processing, Physicochemical, Nutritional, and Organoleptical Properties of Dackere, an African Cereal/Tuber Food Semolina

Dackere is a granulated food made from tubers or cereals flours by women in rural areas of Northern Cameroon. The processing of the semolina faces some constraints that affect the quality and hence the potential to improve the incomes of producers. The objectives of this work were to identify and characterize the processing steps involved in the production of dackere and evaluate the physicochemical and hedonic characteristics of this food. Four groups of farmers consisting of organized and specialized women in dackere processing were used for this purpose and then followed up in their production site. The results obtained revealed that dackere was manufactured from either tubers or cereals based on the available food materials: sweet potato, cassava, corn, millet, fonio, and mixture of sweet potato/corn. Generally, the processes involved included hydrating the flour with water, granulating manually, steam cooking, and eventually drying. All these processes were done in a non-replicable manner using traditional equipment. The physicochemical characterization revealed that dackeres were in general rich in starch (60.63 to 94.99 g/100 g) and poor in proteins (0.77 to 4.42 g/100 g). Digestible starch varied from 34.9 g/100 g for corn dackere to 66.6 g/100 g for cassava and sweet potatoes dackeres, and the index of whiteness varied from 58.8 for sweet potatoes dackere to 75.8 for cassava and corn dackeres. Significant variations were observed among producers. Consumers more appreciated the sweet potato/corn dackere mixture which was associated by principal component analysis to high swallowing ability, taste, and adherence in mouth. However, they were irregular in size and brown in color, characteristics negatively correlated to acceptability.     

Miniaturization limits for single-chamber micro-solid oxide fuel cells with coplanar electrodes

Single-chamber solid oxide fuel cells with coplanar microelectrodes were operated in methane–air mixtures (R_mix = 2) at 700 °C. The performance of cells with one pair of NiO–YSZ (yttria stabilized zirconia) anode and (La_0.8Sr_0.2)_0.98MnO₃–YSZ cathode, arranged parallel on a YSZ electrolyte substrate, was found to be significantly dependent on the electrode width. For an interelectrode gap of 250 μm, cells with average electrode widths exceeding 850 μm could establish a stable open circuit voltage (OCV) of 0.8 V, while those with widths less than 550 μm could not establish any OCV. In the intermediate range, the cells exhibited significant fluctuations in voltage and power under our testing conditions. This behavior suggests that a lower limit to electrode dimensions exists for cells with single electrode pairs, below which neither a stable difference in oxygen partial pressure, nor an OCV, can be established. Conversely, increasing the electrode width imposes a penalty in the form of an increase in the cell resistance. However, both size limits can be circumvented by employing multiple pairs of microscale electrodes in an interdigitated configuration.     

Performance evaluation of a.c. and d.c. direct coupled photovoltaic water pumping systems

Photovoltaic (PV) water pumping systems have been widely tested and assessed in Indonesia because of the deficiency of potable water in remote areas. Several different PV water pumping systems, such as a.c./d.c. with/without battery and various pumps, have been tested in the field. Further study, evaluation and classification of optimized PV water pumping systems is needed. Three of those pumping systems were installed in Sumba Island in 1983, in three adjacent villages with similar climate and environment conditions. Two of the installations had a.c. direct coupled systems and the other had d.c. The a.c. systems used a 3.65 kW_p PV generator each and the d.c. system used 5.76 kW_p. After comparing the performance data of both systems, it can be concluded that the a.c. system performs better and needs less maintenance on the pump. In May 1987, as a result of this evaluation, the 5.76 kW_p d.c. system was replaced by an a.c. one, and its capacity was increased to 9.22 kW_p. Meanwhile, the 3.65 kW_p a.c. system was increased to 3.88 kW_p.     

Evidence for a transitional period for development of persistence in infant rats.

Conducted 4 experiments with a total of 188 rat pups to test the hypothesis that rat pups can learn persistence at age 11 days but cannot express this learning in their behavior until they are a few days older. Exp I used a space-trial procedure to investigate the relative reinforcing effects of milk suckling, dry suckling, and contact without suckling at ages 11 and 14 days. Exp II used the Exp I procedure, manipulating schedule of reward at 2 ages and under 2 of the reward conditions. Exp III manipulated the dry suckling reward schedule in 11- and 14-day-old pups. Exp IV manipulated partial and continuous reinforcement on Day 11 but tested for persistence on Day 14. Results suggest the existence of a traditional period in neonatal rats for the learning of persistence. Partial reinforcement training with suckling on an anesthetized dam as reward induced greater persistence in extinction of the approach response than did continuous reinforcement in rat pups 14 days old but not in 11-day-old pups. Other aspects of this period of development are discussed. (26 ref) (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Press coverage of public expenditure of Master Settlement Agreement funds: how are non-tobacco control related expenditures represented?

Objective: To examine newspaper reports of Master Settlement Agreement (MSA) allocation decisions outside of tobacco control, focusing on the arguments being put forth in favour of competing claims on MSA funds. The major aims were to identify newsworthy non-tobacco control areas for which MSA funds have been applied and to examine how non-tobacco control spending has been presented through the US press.

Design: A qualitative textual analysis was performed on 94 articles taken from a sample of 322 newspapers over a 12 month period (October 2000 through September 2001) that related to the allocation of MSA funds to non-tobacco control causes. Articles were coded for general content areas of MSA allocation as well as for rhetorical and framing devices employed to explain and justify allocation decisions.

Main outcome measures: (1) Areas of non-tobacco control allocation; (2) rhetorical devices and framing techniques employed in the articles to discuss the allocation.

Results: The analysis identified a wide variety of issues for which MSA funds were being appropriated. Three main frames emerged in relation to justifying or appealing for particular spending decisions: (1) funds should be allocated to the needy and/or the deserving; (2) funds should be spent on state development; (3) funds should be put towards helping the state weather a crisis. Claims for each such issue were made using strategies such as presenting the MSA funds as a "windfall", focusing coverage on the merits of the cause rather than the issue of resource allocation, and stressing links between the particular claim and the stated objectives of the MSA.

Conclusions: Press coverage of MSA non-tobacco control spending suggests that the funds have been quickly formulated as fodder for state spending, rather than to support tobacco control efforts. Thus, caution is required in pursuing settlements with the industry where the objective is better funding for tobacco control efforts, particularly in light of the possibility that press coverage of MSA allocation may actually serve as positive publicity for the tobacco industry.