Evidence for behavioural thermoregulation by the world's largest fish

Many fishes make frequent ascents to surface waters and often show prolonged surface swimming following descents to deep water. This affinity for the surface is thought to be related to the recovery of body heat lost at depth. We tested this hypothesis using data from time–depth recorders deployed on four whale sharks (Rhincodon typus). We summarized vertical movements into bouts of dives and classified these into three main types, using cluster analysis. In addition to day and night ‘bounce’ dives where sharks rapidly descended and ascended, we found a third type: single deep (mean: 340 m), long (mean: 169 min) dives, occurring in daytime with extremely long post-dive surface durations (mean: 146 min). Only sharks that were not constrained by shallow bathymetry performed these dives. We found a negative relationship between the mean surface duration of dives in the bout and the mean minimum temperature of dives in the bout that is consistent with the hypothesis that thermoregulation was a major factor driving use of the surface. The relationship broke down when sharks were diving in mean minimum temperatures around 25°C, suggesting that warmer waters did not incur a large metabolic cost for diving and that other factors may also influence surface use.     

Eco-efficiency analysis for remote area power supply selection in Western Australia

Clean Technologies and Environmental Policy - Remote area power supply (RAPS) systems in Western Australia account for more than 56% of total off-grid electricity supply in Australia and utilise...     

Validation of a Method for Quantitation of Soybean Lectin in Commercial Varieties

Soybean agglutinin (SBA) protein, also known as soybean lectin, is regarded as an anti‐nutrient due to its negative effect on the ability of monogastric animals to gain weight following consumption of raw soybean seed. Historically, SBA has been measured using a time‐consuming and cumbersome hemagglutination procedure. The objective of our research was to obtain a validated methodology that is precise and accurate in the measurement of SBA while allowing minimally equipped laboratories to effectively conduct the analysis, thus our focus was on evaluating an existing commercially available ELISA, an enzyme‐linked‐lectin‐assay (ELLA), and a hybrid ELISA/ELLA. A new ELLA technique that can detect and quantify lectins was chosen and modified specifically for the quantitation of SBA in soybean seed. The proposed ELLA methodology is similar to a standard sandwich ELISA, and uses polyacrylamide‐linked N‐acetylgalactosamine (Gal–NAc–PAA) for a capture phase and the biotinylated version (Gal–NAc–PAA–Biotin) for detection. Based upon the validation data, the ELLA method can precisely and accurately determine soybean lectin levels in soybean seed. The validated ELLA method was used to quantify SBA in nine commercial soybean varieties introduced between 1972 and 2008 and demonstrated that the natural variability of SBA is subject to the effects of genotype and environment.     

Polycarbonate‐based composites reinforced by in situ polytetrafluoroethylene fibrillation: Preparation,thermal and rheological behavior

Fibrillar reinforced composites of polytetrafluoroethylene (PTFE) and polycarbonate (PC) were prepared by in situ fibrillation of PTFE into PC matrix using twin screw extruder. Different samples were obtained by varying the relative ratio between PC and PTFE. The rheological properties of the PC/PTFE composites were found to depend on concentration of the PTFE fibrils. The melt strength analysis in nonisothermal conditions was also studied. The increase in force and decrease in drawability with increasing the PTFE content are associated with the PTFE fibrils formed in situ during the thermomechanical process in twin screw extruder. © 2015 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2015 , 132, 42401.     

A Method for Improving Multivariate Measurement Systems Assessment

Multivariate measurement systems analysis (MSA) is usually performed by designing suitable gauge repeatability and reproducibility (R&R) experiments, ignoring available data generated by the measurement system while used for inspection or process control. This article proposes an approach that, by using the data that are routinely available from the regular operation of the instrument, allows the measurement instrument's current precision to be compared against a benchmark. The proposed method may be appropriately used in an integrated and coordinated manner with the usual multivariate gauge study in the sense that it can be used to assess the stability or a possible deterioration in the precision of the measurement instrument while operational. Therefore, the complementary use of the proposed approach and the traditional multivariate gauge R&R studies can be a useful strategy for improving the overall quality of multivariate measurement systems. Furthermore, because it can be implemented at almost no additional cost, it may be effective in reducing the costs of a multivariate MSA performed with a certain frequency. Copyright © 2014 John Wiley & Sons, Ltd.     

Acute β-Hydroxy-β-Methyl Butyrate Suppresses Regulators of Mitochondrial Biogenesis and Lipid Oxidation While Increasing Lipid Content in Myotubes

Leucine modulates synthetic and degradative pathways in muscle, possibly providing metabolic benefits for both athletes and diseased populations. Leucine has become popular among athletes for improving performance and body composition, however little is known about the metabolic effects of the commonly consumed leucine‐derived metabolite β‐hydroxy‐β‐methyl butyrate (HMB). Our work measured the effects of HMB on metabolic protein expression, mitochondrial content and metabolism, as well as lipid content in skeletal muscle cells. Specifically, cultured C2C12 myotubes were treated with either a control or HMB ranging from 6.25 to 25 μM for 24 h and mRNA and/or protein expression, oxygen consumption, glucose uptake, and lipid content were measured. Contrary to leucine's stimulatory effect on metabolism, HMB‐treated cells exhibited significantly reduced regulators of lipid oxidation including peroxisome proliferator‐activated receptor alpha (PPARα) and PPARβ/δ, as well as downstream target carnitine palmitoyl transferase, without alterations in glucose or palmitate oxidation. Furthermore, HMB significantly inhibited activation of the master regulator of energetics, AMP‐activated protein kinase. As a result, HMB‐treated cells also displayed reduced total mitochondrial content compared with true control or cells equivocally treated with leucine. Additionally, HMB treatment amplified markers of lipid biosynthesis (PPARγ and fatty acid synthase) as well as consistently promoted elevated total lipid content versus control cells. Collectively, our results demonstrate that HMB did not improve mitochondrial metabolism or content, and may promote elevated cellular lipid content possibly through heightened PPARγ expression. These observations suggest that HMB may be most beneficial for populations interested in stimulating anabolic cellular processes.