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71.
Suitability of measuring cadaverine for rapid assessment of bacterial quality of fresh and stale mutton (showing signs of incipient spoilage) was examined. HPTLC for rapid screening and detection and HPLC for accurate quantification was employed. Analysis of over 150 samples of varying degrees of freshness and postmortem age showed that in more than 90% of the samples, no cadaverine was detectable when meat was deemed acceptable with <106 orgs/g. However, when meat showed incipient spoilage, as judged by off odour and dull colour, cadaverine was detected in the range of 1–5 ppm. Such meat samples had a bacterial load of 107 orgs / g or more. HPTLC analysis allowed the simultaneous analysis of 5–6 samples and the total time including extraction was 1.5–2 h. As only easily available chemicals and equipment were required, it was a simple, rapid and inexpensive technique. HPTLC detection of cadaverine can therefore be recommended for the rapid assessment of bacterial quality of mutton and HPLC can be used for accurate quantification. 相似文献
72.
In this paper benchmark numerical results for the one-speed criticality problem with isotropic scattering for the slab and sphere are reported. The Fredholm integral equations of the second kind based on the Case eigenfunction formalism are numerically solved by Neumann iterations with the Double Exponential quadrature. 相似文献
73.
A rat liver cytosolic cholesteryl ester hydrolase (CEH) was purified 12,600-fold by ammonium sulfate precipitation, cation
exchange chromatography and gel permeation high-performance liquid chromatography, with an overall yield of 20%. Its properties
are compared to those of pancreatic CEH, with which it has sometimes been identified. Liver CEH exhibited a single silver
stained band following SDS-polyacrylamide gel electrophoresis (Mr=66 kDa), was activated by 0.5–10 mM taurocholate but was strongly inhibited by higher levels of taurocholate, which activate
pancreatic CEH. Whereas bile salts are known to induce formation of a hexamer of pancreatic CEH, in the current study, 0.5
mM taurocholate dissociated a multimeric form of liver CEH to monomer. Liver CEH did not coelute with pancreatic CEH from
cation exchange and chromatofocusing columns, exhibited no immunoreactivity with anti-rat pancreatic CEH IgG in Western blots,
was not inhibited by anti-rat pancreatic CEH IgG and had a different amino acid composition from pancreatic CEH. In contrast
to liver CEH, which is known to be activated by protein kinases A and C, pancreatic CEH was unaffected by cofactors for protein
kinase A and was inhibited by cofactors for protein kinase C. 相似文献
74.
75.
To solve the non-relativistic time dependent Schrödinger equation using the Lanczos method, Park and Light have provided an approximate expression for the time step for a given accuracy. We provide an exact expression for the time step in terms of the eigenvalues and eigenvectors of the resulting tridiagonal matrix. For two test problems, the values of the time step provided by Park and Light differ significantly from the exact values provided by the present method. We also indicate upper and lower bounds for the time step in terms of the maximum and minimum eigenvalues. These bounds indicate the possibility of using a new time step given by the geometric mean of the eigenvalues of the tridiagonal matrix. 相似文献
76.
77.
78.
Shobha Agrawal V. K. Srivastava G. Jayaraman K. D. P. Nigam 《Polymer-Plastics Technology and Engineering》2013,52(3):357-379
The equations of motion and continuity of a fully developed, steady-state, isothermal, incompressible laminar flow of power law fluids within a toroidal-type coiled tube geometry have been solved numerically using the successive overrelaxation method. Numerical solutions have been obtained for curvature ratios from 5 to 100, Dean number from 1 to 300, and power law index from 0.5 to 1.5. Dean's constraint has been relaxed and the numerical solutions for primary and secondary flow, and the numerical results of the friction factor for different values of Dean number, curvature ratio, and power law index have been computed. The solutions have been carefully checked for accuracy and the results are compared with previous studies. The numerical results are found to be consistent with the published results for the case of Newtonian fluids. The ratio of friction factor of a curved tube to a straight tube increases with the Dean number and also with the power law index. 相似文献
79.
C. Mohankumar C. Arumughan R. Kaleysa raj 《Journal of the American Oil Chemists' Society》1990,67(10):665-669
Development of free fatty acids (FFA) and subsequent deterioration of palm oil quality can be attributed to lipase, an active
hydrolytic enzyme present in the mesocarp of oil palm fruit. A histochemical method was used to localize this enzyme in the
mesocarp. FFA released from endogenous glycerides were precipitated as lead salt. The deposits were located on the membranes
of oil bodies. Enzymatic activity in the mesocarp was also studied under different conditions of fruit storage: ambient temperature,
freezing, etc. The enzyme was active after freezing but less active under ambient conditions of fruit storage. Investigations
on fruit development indicated that the enzyme activity started appearing between 16–20 weeks after anthesis (WAA) which synchronized
with the beginnning of lipid formation. These histochemical studies were supported wity biochemical data on FFA. 相似文献
80.
Advanced technologies of microelectronic device fabrication need accurate determination of process parameters and their analysis. Test structures which represent individual process steps are incorporated in the design. Independent measurements can be carried out on these structures and the information is used as a feedback in order to achieve perfection at different stages of fabrication. This note presents an algorithm for analysis of measurement data and identification of defective sites on the wafer leading to an accurate yield analysis. 相似文献